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Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells
Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells
The rational development of small-molecule degraders (e.g., proteolysis targeting chimeras) remains a challenge as the rat...
Preparation of AIEgen-based near-infrared afterglow luminescence nanoprobes for tumor imaging and image-guided tumor resection
Preparation of AIEgen-based near-infrared afterglow luminescence nanoprobes for tumor imaging and image-guided tumor resection
Fluorescence imaging represents a vital tool in modern biology, oncology and biomedical applications. Afterglow luminescen...
Reprogramming fibroblast into human iBlastoids
Reprogramming fibroblast into human iBlastoids
The study of early human embryogenesis has relied on the use of blastocysts donated to research or simple stem cell cultur...
Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials
Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials
In vivo microscopy of single cells enables following pathological changes in tissues, revealing signaling networks and cel...
Control of DNA replication in vitro using a reversible replication barrier
Control of DNA replication in vitro using a reversible replication barrier
A major obstacle to studying DNA replication is that it involves asynchronous and highly delocalized events. A reversible ...
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins
Single-particle cryo-electron microscopy (cryoEM) provides an attractive avenue for advancing our atomic resolution unders...
Packaging and containerization of computational methods
Packaging and containerization of computational methods
Methods for analyzing the full complement of a biomolecule type, e.g., proteomics or metabolomics, generate large amounts ...
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
Isotopically carbon-labeled α-amino acids are valuable synthetic targets that are increasingly needed in pharmacology...
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
A promising cell-therapy approach for heart failure aims at differentiating human pluripotent stem cells (hPSCs) into func...
Generating human bone marrow organoids for disease modeling and drug discovery
Generating human bone marrow organoids for disease modeling and drug discovery
The bone marrow supports and regulates hematopoiesis, responding to physiological requirements for blood cell production o...
An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells
An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells
Pigs share anatomical and physiological traits with humans and can serve as a large-animal model for translational medicin...
Light-mediated intracellular polymerization
Light-mediated intracellular polymerization
The synthesis of synthetic intracellular polymers offers groundbreaking possibilities in cellular biology and medical rese...
Pan-evolutionary and regulatory genome architecture delineated by an integrated macro- and microsynteny approach
Pan-evolutionary and regulatory genome architecture delineated by an integrated macro- and microsynteny approach
The forthcoming massive genome data generated by the Earth BioGenome Project will open up a new era of comparative genomic...
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS
Genetic interactions have the potential to modulate phenotypes, including human disease. In principle, genome-wide associa...
Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils
Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils
Eosinophils are a class of granulocytes with pleiotropic functions in homeostasis and various human diseases. Nevertheless...
BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response
BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response
Modeling immuno-oncology by using patient-derived material and immune cell co-cultures can advance our understanding of im...
Comprehensive synthesis of Ti3C2Tx from MAX phase to MXene
Comprehensive synthesis of Ti3C2Tx from MAX phase to MXene
MXenes are a large family of two-dimensional materials that have attracted attention across many fields due to their desir...
Detection of colinear blocks and synteny and evolutionary analyses based on utilization of MCScanX
Detection of colinear blocks and synteny and evolutionary analyses based on utilization of MCScanX
As different taxa evolve, gene order often changes slowly enough that chromosomal ‘blocks’ with conserved gene...
Transkingdom Network Analysis (TkNA): a systems framework for inferring causal factors underlying host–microbiota and other multi-omic interactions
Transkingdom Network Analysis (TkNA): a systems framework for inferring causal factors underlying host–microbiota and other multi-omic interactions
We present Transkingdom Network Analysis (TkNA), a unique causal-inference analytical framework that offers a holistic vie...
Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales
Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales
The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling used by c...
Publisher Correction: Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy
Authors and AffiliationsFaculty for Chemistry and Biochemistry, Ruhr-Universität Bochum, Bochum, GermanyG. Heinrich, M. Ko...
Profiling native pulmonary basement membrane stiffness using atomic force microscopy
Profiling native pulmonary basement membrane stiffness using atomic force microscopy
Mammalian cells sense and react to the mechanics of their immediate microenvironment. Therefore, the characterization of t...
Design and fabrication of wearable electronic textiles using twisted fiber-based threads
Design and fabrication of wearable electronic textiles using twisted fiber-based threads
Mono-dimensional fiber-based electronics can effectively address the growing demand for improved wearable electronic devic...
Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets
Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets
Volume electron microscopy is the method of choice for the in situ interrogation of cellular ultrastructure at the nanomet...
High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination
High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination
Engineered by nature, biological entities are exceptional building blocks for biomaterials. These entities can impart enha...
Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy
Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy
The instrumental analysis of reaction mixtures is usually the rate-determining step in the optimization of chemical proces...
Reconstructing phylogenetic trees from genome-wide somatic mutations in clonal samples
Reconstructing phylogenetic trees from genome-wide somatic mutations in clonal samples
Phylogenetic trees are a powerful means to display the evolutionary history of species, pathogens and, more recently, indi...
rMATS-turbo: an efficient and flexible computational tool for alternative splicing analysis of large-scale RNA-seq data
rMATS-turbo: an efficient and flexible computational tool for alternative splicing analysis of large-scale RNA-seq data
Pre-mRNA alternative splicing is a prevalent mechanism for diversifying eukaryotic transcriptomes and proteomes. Regulated...
GlYcoLISA: antigen-specific and subclass-specific IgG Fc glycosylation analysis based on an immunosorbent assay with an LC–MS readout
GlYcoLISA: antigen-specific and subclass-specific IgG Fc glycosylation analysis based on an immunosorbent assay with an LC–MS readout
Immunoglobulin G (IgG) fragment crystallizable (Fc) glycosylation modulates effector functions such as antibody-dependent ...
Web-based multi-omics integration using the Analyst software suite
Web-based multi-omics integration using the Analyst software suite
The growing number of multi-omics studies demands clear conceptual workflows coupled with easy-to-use software tools to fa...
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