SCI时时刷

search
+
Assembly of a stem cell-derived human postimplantation embryo model
Assembly of a stem cell-derived human postimplantation embryo model
The embryonic and extraembryonic tissue interactions underlying human embryogenesis at implantation stages are not current...
Tutorial: a guide to diffusion MRI and structural connectomics
Tutorial: a guide to diffusion MRI and structural connectomics
Diffusion magnetic resonance imaging (dMRI) is a versatile imaging technique that has gained popularity thanks to its sens...
Visualizing epigenetic modifications and their spatial proximities in single cells using three DNA-encoded amplifying FISH imaging strategies: BEA-FISH, PPDA-FISH and Cell-TALKING
Visualizing epigenetic modifications and their spatial proximities in single cells using three DNA-encoded amplifying FISH imaging strategies: BEA-FISH, PPDA-FISH and Cell-TALKING
Epigenetic modifications and spatial proximities of nucleic acids and proteins play important roles in regulating physiolo...
Construction and utilization of a new generation of bacteriophage-based particles, or TPA, for guided systemic delivery of nucleic acids to tumors
Construction and utilization of a new generation of bacteriophage-based particles, or TPA, for guided systemic delivery of nucleic acids to tumors
Successful delivery of nucleic acid therapeutics to diseased sites would present a pivotal advancement in cancer treatment...
Precise tracking of nanoparticles in plant roots
Precise tracking of nanoparticles in plant roots
One of the foremost challenges in nanobiotechnology is obtaining direct evidence of nanoparticles’ absorption and in...
Mapping protein–DNA interactions with DiMeLo-seq
Mapping protein–DNA interactions with DiMeLo-seq
We recently developed directed methylation with long-read sequencing (DiMeLo-seq) to map protein–DNA interactions ge...
A bioswitchable delivery system for microRNA therapeutics based on a tetrahedral DNA nanostructure
A bioswitchable delivery system for microRNA therapeutics based on a tetrahedral DNA nanostructure
As microRNAs (miRNA) regulate almost all physiopathological activities in the human body, miRNA therapeutics that deliver ...
Author Correction: Metagenome analysis using the Kraken software suite
Author notesThese authors contributed equally: Jennifer Lu, Natalia Rincon.Authors and AffiliationsDepartment of Biomedica...
Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes
Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes
Measuring forces within living cells remains a technical challenge. In this Tutorial, we cover the development of hydropho...
Genome-wide analysis of the biophysical properties of chromatin and nuclear proteins in living cells with Hi-D
Genome-wide analysis of the biophysical properties of chromatin and nuclear proteins in living cells with Hi-D
To understand the dynamic nature of the genome, the localization and rearrangement of DNA and DNA-binding proteins must be...
Synthesis of N-heterocycles through alcohol dehydrogenative coupling
Synthesis of N-heterocycles through alcohol dehydrogenative coupling
Nitrogen heterocycles are found in the structures of many biologically important compounds, as well as materials used in t...
Native MS-guided lipidomics to define endogenous lipid microenvironments of eukaryotic receptors and transporters
Native MS-guided lipidomics to define endogenous lipid microenvironments of eukaryotic receptors and transporters
The mammalian membrane is composed of various eukaryotic lipids interacting with extensively post-translationally modified...
Fabrication of cyborg bacterial cells as living cell–material hybrids using intracellular hydrogelation
Fabrication of cyborg bacterial cells as living cell–material hybrids using intracellular hydrogelation
The production of living therapeutics, cell-based delivery of drugs and gene-editing tools and the manufacturing of bio-co...
STRAIGHT-IN: a platform for rapidly generating panels of genetically modified human pluripotent stem cell lines
STRAIGHT-IN: a platform for rapidly generating panels of genetically modified human pluripotent stem cell lines
Targeted integration of large DNA cargoes (>10 kb) or genomic replacements in mammalian cells, such as human plurip...
Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes
Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes
Nanozymes are nanomaterials with enzyme-like catalytic properties. They are attractive reagents because they do not have t...
Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
Complex cellular functions occur via the coordinated formation and dissociation of protein complexes. Functions such as th...
Flux synthesis of two-dimensional covalent organic frameworks
Flux synthesis of two-dimensional covalent organic frameworks
Covalent organic frameworks (COFs) are crystalline porous polymers constructed from organic building blocks into ordered t...
Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Single-cell proteomics by mass spectrometry (MS) allows the quantification of proteins with high specificity and sensitivi...
A network control theory pipeline for studying the dynamics of the structural connectome
A network control theory pipeline for studying the dynamics of the structural connectome
Network control theory (NCT) is a simple and powerful tool for studying how network topology informs and constrains the dy...
Host circuit engagement of human cortical organoids transplanted in rodents
Host circuit engagement of human cortical organoids transplanted in rodents
Human neural organoids represent promising models for studying neural function; however, organoids grown in vitro lack cer...
High-resolution topographic surveying and change detection with the iPhone LiDAR
High-resolution topographic surveying and change detection with the iPhone LiDAR
This paper introduces a comprehensive protocol leveraging open-access techniques to create small- to medium-scale 3D repre...
Quantifying genome-wide transcription factor binding affinities for chromatin using BANC-seq
Quantifying genome-wide transcription factor binding affinities for chromatin using BANC-seq
Transcription factors (TFs) bind specific DNA sequences to regulate transcription. Apart from DNA sequences, local factors...
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics
Rare cells have an important role in development and disease, and methods for isolating and studying cell subsets are ther...
Analysis of nanomaterial biocoronas in biological and environmental surroundings
Analysis of nanomaterial biocoronas in biological and environmental surroundings
A biomolecular coating, or biocorona, forms on the surface of engineered nanomaterials (ENMs) immediately as they enter bi...
Surface nanocoating of bacteria as a versatile platform to develop living therapeutics
Surface nanocoating of bacteria as a versatile platform to develop living therapeutics
Bacteria have been extensively utilized as living therapeutics for disease treatment due to their unique characteristics, ...
In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis
In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis
Human early embryonic development is the cornerstone of a healthy baby. Abnormal early embryonic development may lead to d...
Using clusterProfiler to characterize multiomics data
Using clusterProfiler to characterize multiomics data
With the advent of multiomics, software capable of multidimensional enrichment analysis has become increasingly crucial fo...
OBSERVE: guidelines for the refinement of rodent cancer models
OBSERVE: guidelines for the refinement of rodent cancer models
Existing guidelines on the preparation (Planning Research and Experimental Procedures on Animals: Recommendations for Exce...
Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method
Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method
Seamless site-directed mutagenesis is an important technique for studying protein functions, tuning enzyme catalytic activ...
A multi-glycomic platform for the analysis of food carbohydrates
A multi-glycomic platform for the analysis of food carbohydrates
Carbohydrates comprise the largest fraction of most diets and exert a profound impact on health. Components such as simple...
阅读更多
format_indent_increase