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An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF)
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF)
Deep and accurate proteome analysis is crucial for understanding cellular processes and disease mechanisms; however, it is...
Generating allogeneic CAR-NKT cells for off-the-shelf cancer immunotherapy with genetically engineered HSP cells and feeder-free differentiation culture
Generating allogeneic CAR-NKT cells for off-the-shelf cancer immunotherapy with genetically engineered HSP cells and feeder-free differentiation culture
The clinical potential of current chimeric antigen receptor-engineered T (CAR-T) cell therapy is hampered by its autologou...
Fabrication of high-performance tin halide perovskite thin-film transistors via chemical solution-based composition engineering
Fabrication of high-performance tin halide perovskite thin-film transistors via chemical solution-based composition engineering
Metal halide perovskite semiconductors have attracted considerable attention because they enable the development of device...
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics
Top-down analysis of intact proteins and middle-down analysis of proteins subjected to limited digestion require efficient...
Frequency locked whispering evanescent resonator (FLOWER) for biochemical sensing applications
Frequency locked whispering evanescent resonator (FLOWER) for biochemical sensing applications
Sensitive, rapid and label-free biochemical sensors are needed for many applications. In this protocol, we describe bioche...
Lymphatic collection and cell isolation from mouse models for multiomic profiling
Lymphatic collection and cell isolation from mouse models for multiomic profiling
Premetastatic cancer cells often spread from the primary lesion through the lymphatic vasculature and, clinically, the pre...
Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells
Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells
Glycosylated RNAs (glycoRNAs) have recently emerged as a new class of molecules of substantial interest owing to their pot...
Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography–mass spectrometry
Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography–mass spectrometry
Aminoacyl-tRNA synthetases (aaRSs) provide an essential functional link between an mRNA sequence and the protein it encode...
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers
Individual ion mass spectrometry (I2MS) is the Orbitrap-based extension of the niche mass spectrometry technique known as ...
A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments
A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments
The ability to apply controlled forces to individual molecules or molecular complexes and observe their behaviors has led ...
Author Correction: Production and use of antigen tetramers to study antigen-specific B cells
These authors contributed equally: Allyssa Phelps, Diego Pazos-Castro.Department of Medicine, Schroeder Allergy and Immuno...
Preparing submicrometer crystals for electron diffraction
Preparing submicrometer crystals for electron diffraction
A protocol for the preparation of crystals for microcrystal electron diffraction provides X-ray crystallographers and cryo...
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM
Microcrystal electron diffraction (MicroED) has advanced structural methods across a range of sample types, from small mol...
Author Correction: Purification of tubulin with controlled post-translational modifications by polymerization–depolymerization cycles
Author notesGoran LakisicPresent address: Institut MICALIS, AgroParisTech, Université Paris Saclay, INRA, Jouy-en-Josas, F...
YCharOS protocol for antibody validation
YCharOS protocol for antibody validation
Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions o...
A consensus platform for antibody characterization
A consensus platform for antibody characterization
Antibody-based research applications are critical for biological discovery. Yet there are no industry standards for compar...
Integration of large genetic payloads using prime editing and site-specific integrases
Integration of large genetic payloads using prime editing and site-specific integrases
A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-s...
Precise kilobase-scale genomic insertions in mammalian cells using PASTE
Precise kilobase-scale genomic insertions in mammalian cells using PASTE
Programmable gene integration technologies are an emerging modality with exciting applications in both basic research and ...
Independent SAGE as an example of effective public dialogue on scientific research
Independent SAGE as an example of effective public dialogue on scientific research
The World Health Organization declared COVID-19 to be a public health emergency of international concern on 30 January 202...
Enhancing consistency in brain imaging research for population neuroimaging
Standardized neuroimaging protocols are essential to advancing comparability and replicability. Now, a comprehensive magne...
Versatile MRI acquisition and processing protocol for population-based neuroimaging
Versatile MRI acquisition and processing protocol for population-based neuroimaging
Neuroimaging has an essential role in studies of brain health and of cerebrovascular and neurodegenerative diseases, requi...
An explainable deep learning platform for molecular discovery
An explainable deep learning platform for molecular discovery
Deep learning approaches have been increasingly applied to the discovery of novel chemical compounds. These predictive app...
A multiwell plate approach to increase the sample throughput during tissue clearing
A multiwell plate approach to increase the sample throughput during tissue clearing
Tissue clearing, coupled with immunostaining, enables the transition from two-dimensional to three-dimensional pathology a...
Generation and long-term culture of human cerebellar organoids from pluripotent stem cells
Generation and long-term culture of human cerebellar organoids from pluripotent stem cells
The advancement of research on human cerebellar development and diseases has been hindered by the lack of a cell-based sys...
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ
Structure determines function. The discovery of the DNA double-helix structure revealed how genetic information is stored ...
Synthesis of chiral gold helicoid nanoparticles using glutathione
Synthesis of chiral gold helicoid nanoparticles using glutathione
Chiral plasmonic nanostructures are in high demand because of their unique optical properties, which are applicable to pol...
Author Correction: Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS
Author notesThese authors contributed equally: Wesley E. Robertson, Louise F. H. Funke, Daniel de la Torre, Julius Fredens...
Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding
Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding
Protein–protein interactions underpin nearly all biological processes, and understanding the molecular mechanisms th...
RNA sample optimization for cryo-EM analysis
RNA sample optimization for cryo-EM analysis
RNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily de...
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Superstructures with architectural complexity and unique functionalities are promising for a variety of practical applicat...
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