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RNA sample optimization for cryo-EM analysis
RNA sample optimization for cryo-EM analysis
RNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily de...
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units
Superstructures with architectural complexity and unique functionalities are promising for a variety of practical applicat...
High-throughput glycosaminoglycan extraction and UHPLC-MS/MS quantification in human biofluids
High-throughput glycosaminoglycan extraction and UHPLC-MS/MS quantification in human biofluids
Glycosaminoglycans (GAGs) are linear, unbranched heteropolysaccharides whose structural complexity determines their functi...
Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments
Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments
We are thankful for the support of the Ministry of Science, Research and the Arts of Baden-Württemberg (MWK Baden-Württemb...
Rapid parallel reconstruction and specificity screening of hundreds of T cell receptors
Rapid parallel reconstruction and specificity screening of hundreds of T cell receptors
The ability to screen the reactivity of T cell receptors (TCRs) is essential to understanding how antigen-specific T cells...
Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells
Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells
Durable and functional regeneration of the airway epithelium in vivo with transplanted stem cells has the potential to rec...
Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes
Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes
Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-di...
Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomics
Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomics
Spatial transcriptomics technologies aim to advance gene expression studies by profiling the entire transcriptome with int...
Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins
Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins
Unbiased forward genetic screens have been extensively employed in biological research to elucidate functional genomics. I...
Validation and quantification of peptide antigens presented on MHCs using SureQuant
Validation and quantification of peptide antigens presented on MHCs using SureQuant
Vaccines and immunotherapies that target peptide–major histocompatibility complexes (peptide–MHCs) have the po...
Visualizing plant salt stress with a NaCl-responsive fluorescent probe
Visualizing plant salt stress with a NaCl-responsive fluorescent probe
Salt stress is an adverse environmental condition that harms plant growth and development. The development of salt stress ...
High-throughput capture and in situ protein analysis of extracellular vesicles by chemical probe-based array
High-throughput capture and in situ protein analysis of extracellular vesicles by chemical probe-based array
Extracellular vesicles (EVs) are small particles with phospholipid bilayers that carry a diverse range of cargoes includin...
Outcome devaluation as a method for identifying goal-directed behaviors in rats
Outcome devaluation as a method for identifying goal-directed behaviors in rats
Goal-directed behaviors allow animals to act to satisfy needs and desires. The outcome devaluation task is an effective me...
A mouse model of volumetric muscle loss and therapeutic scaffold implantation
A mouse model of volumetric muscle loss and therapeutic scaffold implantation
Skeletal myofibers naturally regenerate after damage; however, impaired muscle function can result in cases when a promine...
Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
Glycans constitute a significant fraction of biomolecular diversity on cellular surfaces across all kingdoms of life. As t...
Easy and accurate protein structure prediction using ColabFold
Easy and accurate protein structure prediction using ColabFold
Since its public release in 2021, AlphaFold2 (AF2) has made investigating biological questions, by using predicted protein...
Publisher Correction: Generation of ‘semi-guided’ cortical organoids with complex neural oscillations
These authors contributed equally: Michael Q. Fitzgerald, Tiffany Chu.Department of Bioengineering, University of Californ...
kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq
kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq
The term ‘RNA-seq’ refers to a collection of assays based on sequencing experiments that involve quantifying R...
Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis
Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis
Synthetic polypeptides, also known as poly(α-amino acids), have the same polyamide backbone structures as natural pro...
High-confidence and high-throughput quantification of synapse engulfment by oligodendrocyte precursor cells
High-confidence and high-throughput quantification of synapse engulfment by oligodendrocyte precursor cells
Oligodendrocyte precursor cells (OPCs) sculpt neural circuits through the phagocytic engulfment of synapses during develop...
Multiplexed chromatin immunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors
Multiplexed chromatin immunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors
ChIP–seq is a widely used technique for studying histone post-translational modifications and DNA-binding proteins. ...
How to isolate channel-forming membrane proteins using the E. coli expression system
How to isolate channel-forming membrane proteins using the E. coli expression system
The recombinant expression, isolation and characterization of pore-forming proteins is one of the most commonly used strat...
Plant sperm cell sequencing for genome phasing and determination of meiotic crossover points
Plant sperm cell sequencing for genome phasing and determination of meiotic crossover points
Haplotype phasing represents a pivotal procedure in genome analysis, entailing the identification of specific genetic vari...
Use of synthetic circular RNA spike-ins (SynCRS) for normalization of circular RNA sequencing data
Use of synthetic circular RNA spike-ins (SynCRS) for normalization of circular RNA sequencing data
High-throughput RNA sequencing enables the quantification of transcript abundance and the identification of novel transcri...
Divergent synthesis of amino acid-linked O-GalNAc glycan core structures
Divergent synthesis of amino acid-linked O-GalNAc glycan core structures
O-GalNAc glycans, also known as mucin-type O-glycans, are primary constituents of mucins on various mucosal sites of the b...
Droplet-based functional CRISPR screening of cell–cell interactions by SPEAC-seq
Droplet-based functional CRISPR screening of cell–cell interactions by SPEAC-seq
Cell–cell interactions are essential for the function and contextual regulation of biological tissues. We present a ...
CellChat for systematic analysis of cell–cell communication from single-cell transcriptomics
CellChat for systematic analysis of cell–cell communication from single-cell transcriptomics
Recent advances in single-cell sequencing technologies offer an opportunity to explore cell–cell communication in ti...
Publisher Correction: In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag
Author notesSubashika GovindanPresent address: Interfaculty Institute of Bioengineering, EPFL, Lausanne, SwitzerlandAuthor...
Rapid generation of homozygous fluorescent knock-in human cells using CRISPR–Cas9 genome editing and validation by automated imaging and digital PCR screening
Rapid generation of homozygous fluorescent knock-in human cells using CRISPR–Cas9 genome editing and validation by automated imaging and digital PCR screening
We previously described a protocol for genome engineering of mammalian cultured cells with clustered regularly interspaced...
Statistical analysis of feature-based molecular networking results from non-targeted metabolomics data
Statistical analysis of feature-based molecular networking results from non-targeted metabolomics data
Feature-based molecular networking (FBMN) is a popular analysis approach for liquid chromatography–tandem mass spect...
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