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MOF-derived nanoporous carbons with diverse tunable nanoarchitectures
MOF-derived nanoporous carbons with diverse tunable nanoarchitectures
Metal-organic frameworks (MOFs), or porous coordination polymers, are crystalline porous materials formed by coordination ...
Neuroscience robotics for controlled induction and real-time assessment of hallucinations
Neuroscience robotics for controlled induction and real-time assessment of hallucinations
Although hallucinations are important and frequent symptoms in major psychiatric and neurological diseases, little is know...
Synthesis of cyclodextrin derivatives for enantiodifferentiating photocyclodimerization of 2-anthracenecarboxylate
Synthesis of cyclodextrin derivatives for enantiodifferentiating photocyclodimerization of 2-anthracenecarboxylate
Photochemical methods are increasingly being used in organic synthesis. They are especially useful for preparing many comp...
Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags
Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags
Population behavior of signaling molecules on the cell surface is key to their adaptive function. Live imaging of proteins...
Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer
Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer
Identifying metabolites and delineating their immune-regulatory contribution in the tumor microenvironment is an area of i...
Small-animal blood exchange is an emerging approach for systemic aging research
Small-animal blood exchange is an emerging approach for systemic aging research
We describe a small-animal blood exchange approach developed for aging research as an alternative to heterochronic parabio...
Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging
Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging
Single-molecule localization microscopy (SMLM) leverages the power of modern optics to unleash ultra-precise structural na...
Determining small-molecule permeation through lipid membranes
Determining small-molecule permeation through lipid membranes
The passive permeability of cell membranes is of key importance in biology, biomedical research and biotechnology as it de...
Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems
Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems
Development of visceral organs such as the esophagus, lung, liver and stomach are coordinated by reciprocal signaling inte...
Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes
Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes
Volcano plots and activity maps are powerful tools for studying homogeneous catalysis. Once constructed, they can be used ...
Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness
Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness
Underwater superoleophobic materials have attracted increasing attention because of their remarkable potential application...
Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues
Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues
Extracellular vesicles (EVs) are nanoscale vesicles secreted into the extracellular space by all cell types, including neu...
Designing and executing prime editing experiments in mammalian cells
Designing and executing prime editing experiments in mammalian cells
Prime editing (PE) is a precision gene editing technology that enables the programmable installation of substitutions, ins...
Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues
Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues
Brain–immune cross-talk and neuroinflammation critically shape brain physiology in health and disease. A detailed un...
High-throughput proteomic sample preparation using pressure cycling technology
High-throughput proteomic sample preparation using pressure cycling technology
High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteo...
I-TASSER-MTD: a deep-learning-based platform for multi-domain protein structure and function prediction
I-TASSER-MTD: a deep-learning-based platform for multi-domain protein structure and function prediction
Most proteins in cells are composed of multiple folding units (or domains) to perform complex functions in a cooperative m...
Prototype mouse models for researching SEND-based mRNA delivery and gene therapy
Prototype mouse models for researching SEND-based mRNA delivery and gene therapy
One of the major challenges of gene therapy—an approach to treat diseases caused by faulty genes—is a lack of ...
Single-crystal structure determination of nanosized metal–organic frameworks by three-dimensional electron diffraction
Single-crystal structure determination of nanosized metal–organic frameworks by three-dimensional electron diffraction
Metal–organic frameworks (MOFs) have attracted considerable interest due to their well-defined pore architecture and...
A rapid screening platform to coculture bacteria within tumor spheroids
A rapid screening platform to coculture bacteria within tumor spheroids
The prevalence of tumor-colonizing bacteria along with advances in synthetic biology are leading to a new generation of li...
Ligand functionalization of titanium nanopattern enables the analysis of cell–ligand interactions by super-resolution microscopy
Ligand functionalization of titanium nanopattern enables the analysis of cell–ligand interactions by super-resolution microscopy
The spatiotemporal aspects of early signaling events during interactions between cells and their environment dictate multi...
Nanocarriers based on bacterial membrane materials for cancer vaccine delivery
Nanocarriers based on bacterial membrane materials for cancer vaccine delivery
Here we present a protocol for the construction and use of two types of nanocarrier based on bacterial membrane materials ...
The cell-line-derived subcutaneous tumor model in preclinical cancer research
The cell-line-derived subcutaneous tumor model in preclinical cancer research
Tumor-bearing experimental animals are essential for preclinical cancer drug development. A broad range of tumor models is...
Selective footprinting of 40S and 80S ribosome subpopulations (Sel-TCP-seq) to study translation and its control
Selective footprinting of 40S and 80S ribosome subpopulations (Sel-TCP-seq) to study translation and its control
Multiple aspects of mRNA translation are subject to regulation. Here we present a ribosome footprinting protocol to determ...
Scalable tissue labeling and clearing of intact human organs
Scalable tissue labeling and clearing of intact human organs
Advances in tissue labeling and clearing methods include improvement of tissue transparency, better preservation of fluore...
Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq
Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq
Poly(A) tails are added to the 3′ ends of most mRNAs in a non-templated manner and play essential roles in post-tran...
Tutorial: design and execution of CRISPR in vivo screens
Tutorial: design and execution of CRISPR in vivo screens
Here we provide a detailed tutorial on CRISPR in vivo screening. Using the mouse as the model organism, we introduce a ran...
Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions
Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions
Lipidomics studies suffer from analytical and annotation challenges because of the great structural similarity of many of ...
Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system
Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system
Circular RNAs (circRNAs) are covalently enclosed, single-stranded RNAs produced by back-splicing of pre-mRNA exons that ha...
Isotropic imaging across spatial scales with axially swept light-sheet microscopy
Isotropic imaging across spatial scales with axially swept light-sheet microscopy
Light-sheet fluorescence microscopy is a rapidly growing technique that has gained tremendous popularity in the life scien...