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Phylogenetic convergence of phase separation and mitotic function in the disordered protein BuGZ
Phylogenetic convergence of phase separation and mitotic function in the disordered protein BuGZ
Abstract Intrinsically disordered proteins (IDPs) effect biological function despite their sequence-encoded lack of prefer...
A novel and unique ATP hydrolysis to AMP by a human Hsp70 Binding immunoglobin protein (BiP)
A novel and unique ATP hydrolysis to AMP by a human Hsp70 Binding immunoglobin protein (BiP)
Abstract Hsp70s are ubiquitous and highly conserved molecular chaperones. They play crucial roles in maintaining cellular ...
Completing the family of human EH domains: Solution structure of the internal EH domain of γ‐synergin
Completing the family of human EH domains: Solution structure of the internal EH domain of γ‐synergin
Abstract Eps15 homology (EH) domains are universal interaction domains to establish networks of protein-protein interactio...
Atomic View of an Amyloid Dodecamer Exhibiting Selective Cellular Toxic Vulnerability in Acute Brain Slices
Atomic View of an Amyloid Dodecamer Exhibiting Selective Cellular Toxic Vulnerability in Acute Brain Slices
Abstract Atomic structures of amyloid oligomers that capture the neurodegenerative disease pathology are essential to unde...
A novel and unique ATP hydrolysis to AMP by a human Hsp70 BiP
A novel and unique ATP hydrolysis to AMP by a human Hsp70 BiP
Abstract Hsp70s are ubiquitous and highly conserved molecular chaperones. They play crucial roles in maintaining cellular ...
A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins
A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins
Abstract We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is th...
Double mutations far from the active site affect cold‐activity in an Antarctic halophilic β‐galactosidase
Double mutations far from the active site affect cold‐activity in an Antarctic halophilic β‐galactosidase
Abstract The Antarctic haloarchaeon, Halorubrum lacusprofundi, contains a polyextremophilic family 42 β-galactosidas...
Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes
Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes
Abstract Sequence variation in related proteins is an important characteristic that modulates activity and selectivity. An...
Modeling the stimulation by glutathione of the steady state kinetics of an adenosine triphosphate binding cassette transporter
Modeling the stimulation by glutathione of the steady state kinetics of an adenosine triphosphate binding cassette transporter
Abstract We report the steady state ATPase activities of the ATP Binding Cassette (ABC) exporter NaAtm1 in the absence and...
Structure of the human Ccr4‐Not nuclease module using X‐ray crystallography and electron paramagnetic resonance spectroscopy distance measurements
Structure of the human Ccr4‐Not nuclease module using X‐ray crystallography and electron paramagnetic resonance spectroscopy distance measurements
Abstract Regulated degradation of mature, cytoplasmic mRNA is a key step in eukaryotic gene regulation. This process is ty...
The molecular basis for the development of Adult T‐cell leukemia/lymphoma in patients with an IRF4K59R mutation
The molecular basis for the development of Adult T‐cell leukemia/lymphoma in patients with an IRF4K59R mutation
Abstract Interferon regulatory factor 4 (IRF4) is an essential regulator in the development of many immune cells, includin...
Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells
Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells
Abstract In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness....
Thioesterase enzyme families: functions, structures, and mechanisms
Thioesterase enzyme families: functions, structures, and mechanisms
Abstract Thioesterases are enzymes that hydrolyze thioester bonds in numerous biochemical pathways, for example in fatty a...
High‐throughput Cell‐free Screening of Eukaryotic Membrane Protein Expression in Lipidic Mimetics
High‐throughput Cell‐free Screening of Eukaryotic Membrane Protein Expression in Lipidic Mimetics
Abstract Membrane proteins play essential roles in cellular function and metabolism. Nonetheless, biophysical and structur...
Molecular dissection of a dedicated formaldehyde dehydrogenase from Mycobacterium smegmatis
Molecular dissection of a dedicated formaldehyde dehydrogenase from Mycobacterium smegmatis
Abstract Accumulation of formaldehyde, a highly reactive molecule, in the cell is toxic, and requires detoxification for t...
Three‐repeat and four‐repeat Tau isoforms form different oligomers
Three‐repeat and four‐repeat Tau isoforms form different oligomers
Abstract Different tauopathies are characterized by isoform-specific composition of the aggregates found in the brain and ...
Activity and substrate specificity of lytic polysaccharide monooxygenases: an ATR FTIR‐based sensitive assay tested on a novel species from Pseudomonas putida
Activity and substrate specificity of lytic polysaccharide monooxygenases: an ATR FTIR‐based sensitive assay tested on a novel species from Pseudomonas putida
Abstract Pseudomonas putida W619 is a soil Gram-negative bacterium commonly used in environmental studies thanks to its ab...
The Ca2+ response of a smart forisome protein is dependent on polymerization
The Ca2+ response of a smart forisome protein is dependent on polymerization
Abstract Forisomes are giant self-assembling mechanoproteins that undergo reversible structural changes in response to Ca2...
Tools 2022: A compilation of tools for protein science
Tools 2022: A compilation of tools for protein science
Protein Science, the flagship journal of The Protein Society, serves an international forum for publishing original report...
Characterization of protein‐ligand binding interactions of enoyl‐ACP reductase (FabI) by native MS reveals allosteric effects of coenzymes and the inhibitor triclosan
Characterization of protein‐ligand binding interactions of enoyl‐ACP reductase (FabI) by native MS reveals allosteric effects of coenzymes and the inhibitor triclosan
Abstract The enzyme enoyl-ACP reductase (also called FabI in bacteria) is an essential member of the fatty acid synthase I...
Structural and Functional Characterization of Fosfomycin Resistance Conferred by FosB from Enterococcus faecium
Structural and Functional Characterization of Fosfomycin Resistance Conferred by FosB from Enterococcus faecium
Abstract The Gram-positive pathogen Enterococcus faecium is one of the leading causes of hospital-acquired vancomycin resi...
Stimulation of the steady state ATPase kinetics of the ABC exporter NaAtm1 by a transported substrate GSSG
Stimulation of the steady state ATPase kinetics of the ABC exporter NaAtm1 by a transported substrate GSSG
Abstract We report the steady state ATPase activities of the ATP Binding Cassette (ABC) exporter NaAtm1 in the absence and...
Design principles that protect the proteasome from self‐destruction
Design principles that protect the proteasome from self‐destruction
Abstract The proteasome is a powerful intracellular protease that can degrade effectively any protein, self or foreign, fo...
A newly introduced salt bridge cluster improves structural and biophysical properties of de novo TIM barrels
A newly introduced salt bridge cluster improves structural and biophysical properties of de novo TIM barrels
Abstract Protein stability can be fine-tuned by modifying different structural features such as hydrogen-bond networks, sa...
Structural and dynamic studies of the peptidase domain from Clostridium thermocellum PCAT1
Structural and dynamic studies of the peptidase domain from Clostridium thermocellum PCAT1
Abstract The export of antimicrobial peptides is mediated by diverse mechanisms in bacterial quorum sensing pathways. One ...
A New Strategy to Reconcile Amyloid Cross‐seeding and Amyloid Prevention in a Binary System of α‐synuclein Fragmental Peptide and hIAPP
A New Strategy to Reconcile Amyloid Cross‐seeding and Amyloid Prevention in a Binary System of α‐synuclein Fragmental Peptide and hIAPP
Abstract Amyloid cross-seeding and amyloid inhibition are two different research subjects being studied separately for dif...
Crystal structure of semisynthetic obelin‐v
Crystal structure of semisynthetic obelin‐v
Abstract Coelenterazine-v (CTZ-v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence ...
Analysis of protein–ligand interactions from titrations and nuclear magnetic resonance relaxation dispersions
Analysis of protein–ligand interactions from titrations and nuclear magnetic resonance relaxation dispersions
Abstract We present PLIS, a publicly available, open-source software for the determination of protein–ligand dissoc...
Crystal structure of semi‐synthetic obelin‐v
Crystal structure of semi‐synthetic obelin‐v
Abstract Coelenterazine-v (CTZ-v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence ...
Structural characterization of a pathogenic mutant of human protein tyrosine phosphatase PTPN2 (Cys216Gly) that causes very early onset autoimmune enteropathy
Structural characterization of a pathogenic mutant of human protein tyrosine phosphatase PTPN2 (Cys216Gly) that causes very early onset autoimmune enteropathy
Abstract PTPN2 is an important protein tyrosine phosphatase (PTP) that plays a key role in cell signaling. Deletions or in...
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