Comprehensive microcrystal electron diffraction sample preparation for cryo-EM

Kuang, Q. et al. Dead-end complex, lipid interactions and catalytic mechanism of microsomal glutathione transferase 1, an electron crystallography and mutagenesis investigation. Sci. Rep. 7, 7897 (2017).

Article  PubMed  PubMed Central  Google Scholar 

Gonen, T. et al. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438, 633–638 (2005).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hiroaki, Y. et al. Implications of the aquaporin-4 structure on array formation and cell adhesion. J. Mol. Biol. 355, 628–639 (2006).

Article  CAS  PubMed  Google Scholar 

Oshima, A., Tani, K., Hiroaki, Y., Fujiyoshi, Y. & Sosinsky, G. E. Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. Proc. Natl Acad. Sci. USA 104, 10034–10039 (2007).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Oshima, A. et al. Asymmetric configurations and N-terminal rearrangements in connexin26 gap junction channels. J. Mol. Biol. 405, 724–735 (2011).

Article  CAS  PubMed  Google Scholar 

Matricardi, V. R., Moretz, R. C. & Parsons, D. F. Electron diffraction of wet proteins: catalase. Science 177, 268–270 (1972).

Article  CAS  PubMed  Google Scholar 

Hui, S. W. & Parsons, D. F. Electron diffraction of wet biological membranes. Science 184, 77–78 (1974).

Article  CAS  PubMed  Google Scholar 

Dorset, D. L. & Parsons, D. F. Electron diffraction from single, fully-hydrated, ox-liver catalase microcrystals. Acta Crystallogr. A 31, 210–215 (1975).

Article  Google Scholar 

Dorset, D. L. Structural Electron Crystallography. (Springer US, Boston, MA, 1995).

Book  Google Scholar 

Dorset, D. L. & Gilmore, C. J. Direct methods in protein electron crystallography—beef liver catalase in its fully hydrated form at room temperature. Acta Crystallogr. A 55, 448–456 (1999).

Article  PubMed  Google Scholar 

Unwin, P. N. & Henderson, R. Molecular structure determination by electron microscopy of unstained crystalline specimens. J. Mol. Biol. 94, 425–440 (1975).

Article  CAS  PubMed  Google Scholar 

Henderson, R. et al. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213, 899–929 (1990).

Article  CAS  PubMed  Google Scholar 

Taylor, K. A. & Glaeser, R. M. Electron diffraction of frozen, hydrated protein crystals. Science 186, 1036–1037 (1974).

Article  CAS  PubMed  Google Scholar 

Jeng, T.-W. & Chiu, W. Experimental strategy in three-dimensional structure determination of crotoxin complex thin crystal. Ultramicroscopy 13, 27–34 (1984).

Article  CAS  PubMed  Google Scholar 

Stokes, D. L. & Green, M. N. Structure of CaATPase: electron microscopy of frozen-hydrated crystals at 6 Å resolution in projection. J. Mol. Biol. 213, 529–538 (1990).

Article  CAS  PubMed  Google Scholar 

Martynowycz, M. W. & Gonen, T. From electron crystallography of 2D crystals to MicroED of 3D crystals. Curr. Opin. Colloid Interface Sci. 34, 9–16 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nannenga, B. L., Shi, D., Leslie, A. G. W. & Gonen, T. High-resolution structure determination by continuous-rotation data collection in MicroED. Nat. Methods 11, 927–930 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Leslie, A. G. W. The integration of macromolecular diffraction data. Acta Crystallogr. D. Biol. Crystallogr. 62, 48–57 (2006).

Article  PubMed  Google Scholar 

Kabsch, W. Integration, scaling, space-group assignment and post-refinement. Acta Crystallogr. D. Biol. Crystallogr. 66, 133–144 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kabsch, W. XDS. Acta Crystallogr. D. Biol. Crystallogr. 66, 125–132 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sheldrick, G. M. A short history of SHELX. Acta Crystallogr. A 64, 112–122 (2008).

Article  CAS  PubMed  Google Scholar 

Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D. Biol. Crystallogr. 66, 213–221 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Winn, M. D. et al. Overview of the CCP4 suite and current developments. Acta Crystallogr. D. Biol. Crystallogr. 67, 235–242 (2011).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hattne, J. et al. MicroED data collection and processing. Acta Crystallogr. A Found. Adv. 71, 353–360 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Clabbers, M. T. B., Gruene, T., Parkhurst, J. M., Abrahams, J. P. & Waterman, D. G. Electron diffraction data processing with DIALS. Acta Crystallogr. D. Struct. Biol. 74, 506–518 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Winter, G. et al. DIALS: implementation and evaluation of a new integration package. Acta Crystallogr. D. Struct. Biol. 74, 85–97 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Iadanza, M. G. & Gonen, T. A suite of software for processing MicroED data of extremely small protein crystals. J. Appl. Crystallogr. 47, 1140–1145 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Battye, T. G. G., Kontogiannis, L., Johnson, O., Powell, H. R. & Leslie, A. G. W. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta Crystallogr. D. Biol. Crystallogr. 67, 271–281 (2011).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Leslie, A. G. W. & Powell, H. R. Processing diffraction data with mosflm. In Evolving Methods for Macromolecular Crystallography (eds. Read, R. J. & Sussman, J. L.) Vol. 245 41–51 (Springer, Dordrecht, Netherlands, 2007).

Sawaya, M. R. et al. Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED. Proc. Natl Acad. Sci. USA 113, 11232–11236 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Martynowycz, M. W., Clabbers, M. T. B., Hattne, J. & Gonen, T. Ab initio phasing macromolecular structures using electron-counted MicroED data. Nat. Methods 19, 724–729 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nannenga, B. L. & Gonen, T. The cryo-EM method microcrystal electron diffraction (MicroED). Nat. Methods 16, 369–379 (2019).

Article 

留言 (0)

沒有登入
gif