Deniz, A. A., Mukhopadhyay, S. & Lemke, E. A. Single-molecule biophysics: at the interface of biology, physics and chemistry. J. R. Soc. Interface 5, 15–45 (2008).

Article  CAS  PubMed  Google Scholar 

Miller, H. et al. Single-molecule techniques in biophysics: a review of the progress in methods and applications. Rep. Prog. Phys. 81, 024601 (2017).

Article  Google Scholar 

Sweeney, H. L. & Houdusse, A. Structural and functional insights into the myosin motor mechanism. Annu. Rev. Biophys. 39, 539–557 (2010).

Article  CAS  PubMed  Google Scholar 

Mohapatra, S. et al. Single-molecule analysis and engineering of DNA motors. Chem. Rev. 120, 36–78 (2019).

Article  PubMed  Google Scholar 

Seidel, R. & Dekker, C. Single-molecule studies of nucleic acid motors. Curr. Opin. Struct. Biol. 17, 80–86 (2007).

Article  CAS  PubMed  Google Scholar 

Woodside, M. T. & Block, S. M. Reconstructing folding energy landscapes by single-molecule force spectroscopy. Annu. Rev. Biophys. 43, 19–39 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Petrosyan, R., Narayan, A. & Woodside, M. T. Single-molecule force spectroscopy of protein folding. J. Mol. Biol. 433, 167207 (2021).

Article  CAS  PubMed  Google Scholar 

Mukherjee, S. et al. Single-molecule optical tweezers as a tool for delineating the mechanisms of protein-processing mechanoenzymes. ACS Omega 8, 87–97 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Camunas-Soler, J., Ribezzi-Crivellari, M. & Ritort, F. Elastic properties of nucleic acids by single-molecule force spectroscopy. Annu. Rev. Biophys. 45, 65–84 (2016).

Article  CAS  PubMed  Google Scholar 

Yodh, J. G., Schlierf, M. & Ha, T. Insight into helicase mechanism and function revealed through single-molecule approaches. Q. Rev. Biophys. 43, 185–217 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Smith, D. E. Single-molecule studies of viral DNA packaging. Curr. Opin. Virol. 1, 134–141 (2011).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dulin, D. et al. Studying genomic processes at the single-molecule level: introducing the tools and applications. Nat. Rev. Genet. 14, 9–22 (2013).

Article  CAS  PubMed  Google Scholar 

Evans, E. Probing the relation between force—lifetime—and chemistry in single molecular bonds. Annu. Rev. Biophys. Biomol. Struct. 30, 105–128 (2001).

Article  CAS  PubMed  Google Scholar 

Puchner, E. M. & Gaub, H. E. Single-molecule mechanoenzymatics. Annu. Rev. Biophys. 41, 497–518 (2012).

Article  CAS  PubMed  Google Scholar 

Yusko, E. C. & Asbury, C. L. Force is a signal that cells cannot ignore. Mol. Biol. Cell 25, 3717–3725 (2014).

Article  PubMed  PubMed Central  Google Scholar 

Eckels, E. C. et al. The work of titin protein folding as a major driver in muscle contraction. Annu. Rev. Physiol. 80, 327–351 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Neuman, K. C. & Nagy, A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat. Methods 5, 491–505 (2008).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Moffitt, J. R. et al. Recent advances in optical tweezers. Annu. Rev. Biochem. 77, 205–228 (2008).

Article  CAS  PubMed  Google Scholar 

Polimeno, P. et al. Optical tweezers and their applications. J. Quant. Spectrosc. Radiat. Transf. 218, 131––150 (2018).

Article  Google Scholar 

Bustamante, C. J. et al. Optical tweezers in single-molecule biophysics. Nat. Rev. Methods Prim. 1, 25 (2021).

Article  CAS  Google Scholar 

De Vlaminck, I. & Dekker, C. Recent advances in magnetic tweezers. Annu. Rev. Biophys. 41, 453–472 (2012).

Article  PubMed  Google Scholar 

Kilinc, D. & Lee, G. U. Advances in magnetic tweezers for single molecule and cell biophysics. Integr. Biol. 6, 27–34 (2014).

Article  CAS  Google Scholar 

Choi, H.-K. et al. High-resolution single-molecule magnetic tweezers. Annu. Rev. Biochem. 91, 33–59 (2022).

Article  CAS  PubMed  Google Scholar 

Engel, A. & Müller, D. J. Observing single biomolecules at work with the atomic force microscope. Nat. Struct. Biol. 7, 715–718 (2000).

Article  CAS  PubMed  Google Scholar 

Hinterdorfer, P. & Dufrêne, Y. F. Detection and localization of single molecular recognition events using atomic force microscopy. Nat. Methods 3, 347–355 (2006).

Article  CAS  PubMed  Google Scholar 

Parot, P. et al. Past, present and future of atomic force microscopy in life sciences and medicine. J. Mol. Recogniti. 20, 418–431 (2007).

Article  CAS  Google Scholar 

Ma, L. & Cockroft, S. L. Biological nanopores for single‐molecule biophysics. ChemBioChem 11, 25–34 (2010).

Article  CAS  PubMed  Google Scholar 

Sitters, G. et al. Acoustic force spectroscopy. Nat. Methods 12, 47–50 (2015).

Article  CAS  PubMed  Google Scholar 

Gourier, C. et al. A nanospring named erythrocyte. The biomembrane force probe. Cell. Mol. Bioeng. 1, 263–275 (2008).

Article  Google Scholar 

Nickels, P. C. et al. Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp. Science 354, 305–307 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kim, S. et al. Multiplexed single-molecule assay for enzymatic activity on flow-stretched DNA. Nat. Methods 4, 397–399 (2007).

Article  CAS  PubMed  Google Scholar 

Akbari, E. et al. Low cost and massively parallel force spectroscopy with fluid loading on a chip. Nat. Commun. 13, 6800 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Halvorsen, K. & Wong, W. P. Massively parallel single-molecule manipulation using centrifugal force. Biophys. J. 98, L53––L55 (2010).

Article  PubMed  PubMed Central  Google Scholar 

Yang, D. et al. Multiplexed single-molecule force spectroscopy using a centrifuge. Nat. Commun. 7, 11026 (2016).