SCI时时刷

search
+
Hashing the message with cells
Hashing the message with cells
The programming of multicellular processes for biological computation requires increasingly complex genetic circuit design...
Continuous selection in E. coli to identify cyclic peptide inhibitors of protein aggregation
Continuous selection in E. coli to identify cyclic peptide inhibitors of protein aggregation
Targeting of protein aggregates is technologically challenging. We developed a phage-assisted continuous evolution platfor...
Small molecule inhibits KCNQ channels with a non-blocking mechanism
Small molecule inhibits KCNQ channels with a non-blocking mechanism
Voltage-gated ion channels (VGICs) are crucial targets for neuropsychiatric therapeutics owing to their role in controllin...
PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins
PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins
Manipulating viral protein stability using the cellular ubiquitin-proteasome system (UPS) represents a promising approach ...
Aromatic side-chain crosslinking in RiPP biosynthesis
Aromatic side-chain crosslinking in RiPP biosynthesis
Peptide cyclization is a defining feature of many bioactive molecules, particularly in the ribosomally synthesized and pos...
Orthogonalized human protease control of secreted signals
Orthogonalized human protease control of secreted signals
Synthetic circuits that regulate protein secretion in human cells could support cell-based therapies by enabling control o...
A DNA phosphorothioation pathway via adenylated intermediate modulates Tdp machinery
A DNA phosphorothioation pathway via adenylated intermediate modulates Tdp machinery
In prokaryotes, the non-bridging oxygen in the DNA sugar-phosphate backbone can be enzymatically replaced by a sulfur atom...
Catalytic-independent functions of the Integrator–PP2A complex (INTAC) confer sensitivity to BET inhibition
Catalytic-independent functions of the Integrator–PP2A complex (INTAC) confer sensitivity to BET inhibition
Chromatin and transcription regulators are critical to defining cell identity through shaping epigenetic and transcription...
Synergistic protection of nascent DNA at stalled forks by MSANTD4 and BRCA1/2–RAD51
Synergistic protection of nascent DNA at stalled forks by MSANTD4 and BRCA1/2–RAD51
The regressed arms of reversed replication forks exhibit structural similarities to one-ended double-stranded breaks and n...
Transforming an ATP-dependent enzyme into a dissipative, self-assembling system
Transforming an ATP-dependent enzyme into a dissipative, self-assembling system
Nucleoside triphosphate (NTP)-dependent protein assemblies such as microtubules and actin filaments have inspired the deve...
A cell-free biosensor signal amplification circuit with polymerase strand recycling
A cell-free biosensor signal amplification circuit with polymerase strand recycling
Cell-free systems are powerful synthetic biology technologies that can recapitulate gene expression and sensing without th...
Rapid discovery of cyclic peptide protein aggregation inhibitors by continuous selection
Rapid discovery of cyclic peptide protein aggregation inhibitors by continuous selection
Protein aggregates are associated with numerous diseases. Here we report a platform for the rapid phenotypic selection of ...
Abiotic catalysis promoted by liquid–liquid phase separation
Abiotic catalysis promoted by liquid–liquid phase separation
By placing artificial metalloenzymes (ArMs) in phase-separated sanctuary regions formed by their protein scaffolds in Esch...
Chemically engineered antibodies for autophagy-based receptor degradation
Chemically engineered antibodies for autophagy-based receptor degradation
Cell surface receptor-targeted protein degraders hold promise for drug discovery. However, their application is restricted...
The molecular mechanism of temperature-dependent phase separation of heat shock factor 1
The molecular mechanism of temperature-dependent phase separation of heat shock factor 1
Heat shock factor 1 (HSF1) is the critical orchestrator of cell responses to heat shock, and its dysfunction is linked to ...
FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains
FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains
O-Fucosylation plays crucial roles in various essential biological events. Alongside the well-established O-fucosylation o...
Molecular mechanisms of inverse agonism via κ-opioid receptor–G protein complexes
Molecular mechanisms of inverse agonism via κ-opioid receptor–G protein complexes
Opioid receptors, a subfamily of G protein-coupled receptors (GPCRs), are key therapeutic targets. In the canonical GPCR a...
Mechanisms of metabolism-coupled protein modifications
Mechanisms of metabolism-coupled protein modifications
Intricate coupling between metabolism and protein post-translational modifications (PTMs) has emerged as a fundamental asp...
Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI
Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI
Ribozymes that catalyze site-specific RNA modification have recently gained increasing interest for their ability to mimic...
Artificial metalloenzyme assembly in cellular compartments for enhanced catalysis
Artificial metalloenzyme assembly in cellular compartments for enhanced catalysis
Artificial metalloenzymes (ArMs) integrated within whole cells have emerged as promising catalysts; however, their sensiti...
Population-level amplification of gene regulation by programmable gene transfer
Population-level amplification of gene regulation by programmable gene transfer
Engineering cells to sense and respond to environmental cues often focuses on maximizing gene regulation at the single-cel...
Diary of a cell in DNA ‘chyrons’
Diary of a cell in DNA ‘chyrons’
Cellular recording in DNA memory is an emerging and promising strategy to document the biological activity of individual c...
Engineering covalent small molecule–RNA complexes in living cells
Engineering covalent small molecule–RNA complexes in living cells
Covalent labeling of RNA in living cells poses many challenges. Here we describe a structure-guided approach to engineer c...
E2–Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells
E2–Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells
The E2 ubiquitin (Ub)-conjugating enzyme primarily determines Ub conjugation as Ub-isopeptide (lysine), Ub-oxyester (serin...
Programmable trans-splicing riboregulators for complex cellular logic computation
Programmable trans-splicing riboregulators for complex cellular logic computation
Synthetic genetic circuits program the cellular input–output relationships to execute customized functions. However,...
Community-specific cell death sustains bacterial expansion under phosphorus starvation
Community-specific cell death sustains bacterial expansion under phosphorus starvation
Colony expansion is important for establishing territories. It is unclear to what extent bacteria can maintain colony expa...
A speedy cocktail
A speedy cocktail
Human induced pluripotent stem cells reprogrammed from somatic cells by small molecules represent a promising approach for...
A rapid chemical reprogramming system to generate human pluripotent stem cells
A rapid chemical reprogramming system to generate human pluripotent stem cells
Chemical reprogramming enables the generation of human pluripotent stem (hCiPS) cells from somatic cells using small molec...
The impact of library size and scale of testing on virtual screening
The impact of library size and scale of testing on virtual screening
Virtual ligand libraries for ligand discovery have recently increased 10,000-fold. Whether this has improved hit rates and...
Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells
Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells
Directed evolution in mammalian cells offers a powerful approach for advancing synthetic biology applications. However, ex...
阅读更多
format_indent_increase