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Quantification of six types of cytokinins: Integration of an ultra-performance liquid chromatographic-electrospray tandem mass spectrometric method with antibody based immunoaffinity columns equally recognizing cytokinins in free base and nucleoside forms
Cytokinins (CTKs) exist in various types in plants. The accurate quantification of free base and nucleoside types of cytok...
High separation performance of carbon dioxide-based poly(ether-carbonate) copolymer for gas chromatographic analyses
Volume 1682, 25 October 2022, 463493Highlights•Carbon dioxide-based poly(ether-carbonate) copolymers.•High-resolution sepa...
Poly(carboxyethyl acrylate-co-ethylene glycol dimethacrylate) precursor monolith with bonded anthracenyl ligands for use in reversed-phase capillary electrochromatography based on hydrophobic and π-π interactions
Volume 1682, 25 October 2022, 463526Highlights•Expanding the potentials of CEC to the analysis of various aromatic compoun...
Changes in antibody binding and functionality after humanizing a murine scFv anti-IFN-α2: From in silico studies to experimental analysis
The structural and dynamic changes introduced during antibody humanization continue to be a topic open to new contribution...
Paper of the year
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The role of cuticle in fruit shelf-life
Ensuring the availability of high-quality fresh fruits requires the development of strategies to maintain prolonged shelf-...
Hexagonal boron nitride nanosheets based magnetic solid phase extraction for the extraction of phenoxy carboxylic acid herbicides from water samples followed by high-performance liquid chromatography-tandem mass spectrometry
Volume 1682, 25 October 2022, 463519Highlights•A novel Fe3O4@h-BNNSs was prepared and applied for the analysis of PCA herb...
Determination of trace phenoxy carboxylic acid herbicides in environmental water samples by covalent organic frameworks based solid phase extraction coupled with liquid chromatography-tandem mass spectrometry
Volume 1682, 25 October 2022, 463516Highlights•TAPA-TFPB-COFs was simply synthesized at room temperature.•TAPA-TFPB-COFs w...
Polyelectrolyte-grafted anion exchangers with hydrophilic intermediate layers for ion chromatography
Volume 1682, 25 October 2022, 463498Highlights•Polyelectrolyte-grafted anion exchangers with underlying hydrophilic layers...
A method of isolating and analysing drugs from cancer cells for preclinical research
Volume 1682, 25 October 2022, 463500Highlights•Analysis of drug absorption and retention in cancer cells for preclinical s...
Recent advances in capillary gel electrophoresis for the analysis of proteins
Volume 1682, 25 October 2022, 463453Highlights•In-depth information about the technology and the current state of the art....
Dual switch simulated moving bed chromatography: An optimal two‐fraction yielding separation process
Volume 1682, 25 October 2022, 463449Highlights•Novel Dual Switch SMBC yields two fractions for both, extract and raffinate...
Early-Career Complementologists (ECCO) – Past achievements and future directions
The Early-Career Complementologists (ECCO) is a task force that was established, in close collaboration with the European ...
Evaluating the clinical utility of measuring levels of factor H and the related proteins
After years of disappointing clinical results, the tide has finally changed and complement targeted-therapies have become ...
circVMA21 combining with TAF15 stabilizes SOCS3 mRNA to relieve septic lung injury through regulating NF-κB activation
BackgroundLung injury is a severe complication of sepsis, which brings great threats and challenges to human health. CircV...
Residue interaction network and molecular dynamics simulation study on the binding of S239D/I332E Fc variant with enhanced affinity to FcγRIIIa receptor
Engineering of Fc has been adapted as an efficient method for enhanced or reduced affinity towards Fc receptors in the dev...
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals
The objective of this cross-sectional study was to determine the accuracy of the digital Brix and serum total protein (TP)...
Mathematical modeling and process analytical technology for continuous chromatography of biopharmaceutical products
Column chromatography is undeniably the industry standard for the purification of biotherapeutics beginning with the produ...
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods
Bacteriophages (also known as phages) are bacterial viruses that specifically infect and kill bacteria. Use of phages to c...
Building mutational bridges between carbohydrate-active enzymes
To support the central role that carbohydrates and their derivatives play in the living world, Nature has evolved a large ...
Obtaining clean and informative mass spectra from complex chromatographic and high-resolution all-ions-fragmentation data by nonnegative parallel factor analysis 2
A major challenge in processing of complex data obtained from chromatography hyphenated to mass spectrometry is to resolve...
Preparation and application of nano petal-shaped covalent organic frameworks modified polystyrene-divinylbenzene- glycidylmethacrylate microspheres for the extraction of illicit drugs from wastewater
Volume 1682, 25 October 2022, 463505Highlights•The novel [email protected]/DVB/GMA microspheres have been designed and syn...
Application of advanced machine learning algorithms for anomaly detection and quantitative prediction in protein A chromatography
Volume 1682, 25 October 2022, 463486Highlights•Advanced ML algorithms for column integrity breach and yield prediction hav...
Retention behaviors of pre-column derivatized mono-, di- and oligosaccharides in various modes of high performance liquid chromatography
Volume 1682, 25 October 2022, 463508Highlights•Various HPLC modes for the analysis of mono- and oligosaccharides.•Precolum...
Extending the performance of FcRn and FcγRIIIa affinity liquid chromatography for protein biopharmaceuticals
Volume 1682, 25 October 2022, 463518HIGHLIGHTS•Affinity liquid chromatography using FcRn and FcγRIIIa columns was critical...
Nontarget analysis and characterization of alkylamides in electrical product plastics by gas chromatography-positive chemical ionization quadrupole-orbitrap high-resolution mass spectrometry and quasi-molecular ion screening and anchoring algorithm
Volume 1682, 25 October 2022, 463466Highlights•A nontarget analysis method for alkylamides by GC-PCI-Q-Orbitrap-HRMS was d...
On-line trace monitoring of volatile halogenated compounds in air by improved thermal desorption-gas chromatography-mass spectrometry
Volume 1682, 25 October 2022, 463507Highlights•Novel method was developed for ultratrace volatile halogenated compounds me...
Preparation of nickel-chelated iminodiacetate-functionalized macroporous agarose monolith using modular and clickable building blocks for affinity separation of histidine-tagged recombinant proteins
Volume 1682, 25 October 2022, 463509Highlights•Clickable reaction enables fast and straightforward polymer immobilization....
Maigret’s zinc phosphide challenge
Maigret’s zinc phosphide challenge
If Sherlock Holmes is England’s most famous fictional detective, then there is little doubt that Inspector Maigret holds a...
Affinity-based isolation of extracellular vesicles and the effects on downstream molecular analysis
Affinity-based isolation of extracellular vesicles and the effects on downstream molecular analysis
Extracellular vesicles (EVs) are transport vesicles with diameters ranging from 30 to 1000 nm, secreted by cells ...
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