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Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development
Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a virus of zoonotic origin initially identified in Decembe...
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response
The hypersensitivity reactions, triggered by the immune responses directed to the allergens in Hymenoptera venoms, are one...
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-Plex analysis
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-Plex analysis
In late December 2019, 41 cases of pneumonia with an unknown cause were reported in the city of Wuhan (Lu et al., 2020a). ...
Kinetic titration method in flow cytometry for quantification of cell receptors
Kinetic titration method in flow cytometry for quantification of cell receptors
Fluorescence flow cytometry (Robinson and Roederer, 2015; Sklar and Finney, 1982; Stelzer, 1988; Giesecke et al., 2017) is...
Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)
Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)
Black carp (Mylopharyngodon piceus) belongs to the Cyprinidae family and is one of the “four major Chinese carps” in fresh...
An enzyme-free method for isolating testicular macrophages from rodent models
An enzyme-free method for isolating testicular macrophages from rodent models
Macrophages are the major type of immune cell in the testis of both humans and rodents. Testicular macrophages (TMs) play ...
Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists
Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists
Toll-like receptors (TLRs) are type I transmembrane glycoproteins expressed on the membrane of leukocytes, T cells, B cell...
Development of a concise and reliable method for quantifying the antibody loaded onto lipid nanoparticles modified with Herceptin
Development of a concise and reliable method for quantifying the antibody loaded onto lipid nanoparticles modified with Herceptin
Antibodies, also known as immunoglobulins, are glycoproteins initially produced in response to foreign substances or antig...
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff
KeywordsTarget protein-collision immunofixation electrophoresis reflex assayM-proteinTherapeutic monoclonal antibodyImmuno...
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer
Cellular therapy workflows typically involve numerous bioprocessing steps for incoming patient samples in both development...
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation
Xenotransplantation—the process of transplanting organs, cells, or tissues from one species to another—has the potential t...
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation
Antibodies (Abs) are essential tools in biomedical research for the detection and quantification of target proteins (Goldm...
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry
The growing incidence of Streptococcus pneumoniae infection (Karcic et al., 2015) highlights the urgent need for vaccines ...
Performance comparison of three commercial tests for the detection of SARS-CoV-2 antibodies in a common set of pediatric samples
Performance comparison of three commercial tests for the detection of SARS-CoV-2 antibodies in a common set of pediatric samples
Serologic diagnosis of COVID-19 is useful for epidemiologic studies and to support diagnosis of patients with post-acute p...
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
Multiple Sclerosis (MS) is an autoimmune disease of the central nervous system that typically manifests in the 2nd, 3rd or...
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
Growth stimulating express gene 2 (ST2) is also known as suppression of tumorigenicity 2, sulfotransferase homolog 2, and ...
Corrigendum to “A taxonomy of multiple regression methods for immunologists” [Journal of Immunological Methods (2023) pp. 1–6 / Article No. 113506]
Corrigendum to “A taxonomy of multiple regression methods for immunologists” [Journal of Immunological Methods (2023) pp. 1–6 / Article No. 113506]
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Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that affects multiple physiological and pathological functions i...
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)
Available online 16 July 2023, 113524Author links open overlay panel, , , , Highlights•New resources for a budding immunol...
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
The latex turbidimetric immunoassay (LTIA), based on antigen-dependent specific aggregation of antibody-immobilized latex ...
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
Lumpy skin disease (LSD) is characterized by fever, enlargement of lymph nodes, anorexia, discharge from the eyes/nose and...
Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies
KeywordsDried serum spotsPre-punched filter paper discsSubmerge and dry protocolPatient serumAntibody analysisAntigen anal...
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
Lumpy skin disease (LSD) is characterized by fever, enlargement of lymph nodes, anorexia, discharge from the eyes/nose and...
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells
AIMActivation induced markersCHLAChildren's Hospital Los AngelesCOVID-19Coronavirus disease 2019EDTAEthylenediaminetetraac...
A time-resolved fluorescence immunoassay for rapid and precise automatic quality control of human papillomavirus type 68 VLPs in human papillomavirus vaccine
Human papillomavirus (HPV) is a small, non-enveloped deoxyribonucleic acid (DNA) virus that causes infection of skin or mu...
PLGA nanoparticles containing Intimin-Flagellin fusion protein for E. coli O157:H7 nano-vaccine
Hemolytic uremic syndrome (HUS) is the most prevalent and potentially fatal complication associated with Shiga toxin produ...
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids
Streptococcus pneumoniae is one of the most common microorganisms causing acute otitis media (AOM) in children. While bact...
Impact of delayed PBMC processing on functional and genomic assays
Blood samples are routinely collected from clinical trial participants to improve the understanding of the biology of dise...
IFN-γ secretion of PBMC from non-drug-allergic control persons: Considerations for the validity of a positive lymphocyte transformation test
The lymphocyte transformation test (LTT) is used for the in vitro detection of a drug sensitization in assumed drug allerg...
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay
Echinococcosis is a unique parasitic disease that is endemic in many countries of the world, particularly in developing co...
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