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An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination
An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination
The T cell receptor (TCR)-CD3 complex consists of the antigen binding subunit TCRαβ and three CD3 heterodimers, including ...
Selection of positive controls and their impact on anti-drug antibody assay performance
Selection of positive controls and their impact on anti-drug antibody assay performance
Biologic drugs have the potential to induce adaptive immune responses, resulting in the formation of anti-drug antibodies ...
Performance of Softcup® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
Performance of Softcup® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
The field of mucosal immunology research has expanded significantly over the years and understanding biomarkers as correla...
High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
Epitope mapping provides critical insight into antibody-antigen interactions. Epitope mapping of autoantibodies from patie...
An immunochromatographic test for serological diagnosis of scrub typhus
An immunochromatographic test for serological diagnosis of scrub typhus
Scrub typhus is an acute febrile human disease caused by mite vectors, which are infected by a gram negative bacterium O. ...
Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
T lymphocytes are a fundamental component of the human immune system, providing protection against invading pathogens and ...
True volumetric counting of CD34+ cells using flow cytometry
True volumetric counting of CD34+ cells using flow cytometry
In 1957, Edward Donnall Thomas reported the first successful bone marrow transplant on a patient (Thomas et al., 1957). Ov...
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay
The biological and physiological changes in the human body constitute a process characterized by the aging phenomenon, inf...
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel
Cookies are used by this site. Cookie SettingsAll content on this site: Copyright © 2024 Elsevier B.V., its licensors, an...
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B
Available online 6 February 2024, 113628Author links open overlay panel, , AbstractThe importance of structural genetic va...
Comparison of three methods for the detection of antibodies against muscle-specific kinase
Comparison of three methods for the detection of antibodies against muscle-specific kinase
Myasthenia gravis (MG) is an autoimmune disorder characterized by fatigable skeletal muscle weakness. This can manifest in...
Methods for studying P2X4 receptor ion channels in immune cells
Methods for studying P2X4 receptor ion channels in immune cells
Ion channels play essential roles in immune cells, inflammation and immunity (Feske et al., 2015). Among these is the P2X4...
Immunofluorescence analysis of human eosinophils
Immunofluorescence analysis of human eosinophils
Eosinophils are granulated white blood cells that play an important role in mediating host immunity against fungal, bacter...
Stability of anti-drug antibodies in human serum samples
Stability of anti-drug antibodies in human serum samples
Anti-drug antibodies (ADAs) can be induced in vivo in individuals treated with biotherapeutics. Detection of ADAs and eval...
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade
T cells engineered to express engineered T cell receptor (eTCR) or chimeric antigen receptor (CAR) constructs have become ...
A live mammalian cells electroporation array for on-chip immunofluorescence
A live mammalian cells electroporation array for on-chip immunofluorescence
Immunofluorescence is a widely used technique to detect proteins inside cells or tissues in vitro using antibodies. Due to...
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
CAR-T cell immunotherapy is a promising anticancer treatment that redirects the host's immune system against the cancer ce...
Dissociation of murine oral mucosal tissues for single cell applications
Dissociation of murine oral mucosal tissues for single cell applications
The oral mucosa is a barrier site which is under constant external challenges, including a diverse oral microbiome and mec...
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells
Rhesus macaques (RMs) are valuable large-animal models for studying human pathogens, providing insights into protective im...
Variegate expression of Cre recombinase in hematopoietic cells in CD11c-cre transgenic mice
Variegate expression of Cre recombinase in hematopoietic cells in CD11c-cre transgenic mice
Mice genetically engineered to express Cre recombinase under the control of a tissue- or cell type-specific promoter are p...
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response
Available online 12 December 2023, 113601Author links open overlay panel, , , , , AbstractA tumor nano-lysate “TNL” vaccin...
Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen
Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen
Intestinal transplantation (ITx) is the definitive treatment for intestinal failure (Sudan et al., 2000; Kaufman et al., 2...
In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
Borrelia burgdorferi sensu lato (s.l.) is a group of gram-negative bacteria that are the etiological factor of Lyme diseas...