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Use of capillary-mediated vitrification to produce thermostable, single-use antibody conjugates as immunoassay reagents
The performance of enzyme-linked immunoassays is directly dependent on the storage, handling, and long-term stability of t...
Genetic fusion of mussel foot protein to ZZ protein improves target detection in solid-phase immunoassays
In the process of a solid-phase immunoassay, the stability and binding orientation between the antibody and the solid matr...
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue
We are having a few technical issues and our site is currently unavailable. We are working to get it back online as qui...
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia
Available online 14 March 2023, 113458Author links open overlay panel, , AbstractMetalloproteases derived from microbial p...
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials
Qualified researchers may request access to patient-level data and related documents [including, e.g., the clinical study ...
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR
Background and objectiveOverexpression of EGFR, a member of the ErbB receptor family, has been observed in several cancers...
Identification and characterization of phage display-selected peptides having affinity to Peste des petits ruminants virus
Phage display is a well-established technique used for selecting novel ligands having affinity to a plethora of targets in...
An optimized flow cytometry protocol for simultaneous detection of T cell activation induced markers and intracellular cytokines: Application to SARS-CoV-2 immune individuals
Antigen (ag)-specific T cell analysis is an important step for investigation of cellular immunity in many settings, such a...
Optimizing the viability, stability, and potency of Buffy coat isolated T cells for homologous dendritic cell co-cultures: A method for handling and preservation
The utilization of T cells is becoming increasingly prominent in both clinical and research domains. However, the need to ...
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1
AMLAcute Myeloid LeukemiaBDBecton, Dickinson and CompanyCCL18C-C motif chemokine ligand 18CCL22C-C motif chemokine ligand ...
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays
The use of conventional serum for supplementation of media in cell-based and single-cell functional assays has been a majo...
A modified ELISA assay differentiates CCL20 locked dimers from wild-type monomers
A novel engineered CCL20 locked dimer (CCL20LD) is nearly identical to the naturally occurring chemokine CCL20 but blocks ...
A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen
Flow cytometry (FC) is a highly informative technology that can provide valuable information about immune phenotype monito...
An optimized flow cytometry protocol for simultaneous detection of T cell activation induced markers and intracellular cytokines: Application to SARS-CoV2 immune individuals
Antigen (ag)-specific T cell analysis is an important step for investigation of cellular immunity in many settings, such a...
Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node
Available online 25 February 2023, 113445Author links open overlay panel, , , , , , , , , , , , , , , AbstractMany HIV-1 v...
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus
The RNA synthesis of porcine epidemic diarrhea virus (PEDV) is a sophisticated process performed by a multilingual viral r...
Optimized flow cytometry panel for the detection and analysis of human tumor-induced memory-like NK cells
Available online 8 February 2023, 113439Author links open overlay panel, , , , AbstractRecent studies indicate that under ...
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute
Canine distemper is an emerging disease, caused by the Canine morbillivirus (CDV) of the Paramyxoviridae family. The virus...
Elevation of truncated (48 kDa) form of unconventional myosin 1C in blood serum correlates with severe Covid-19
In Covid-19 and autoimmune patients, there are several similarities revealed in the immune responses (Liu et al., 2021; Wo...
Drug-tolerant detection of anti-drug antibodies in an antigen-binding assay using europium chelate fluorescence
Available online 28 January 2023, 113436Author links open overlay panelHighlights•Accurate anti-drug antibody (ADA) detect...
Letter to the editor regarding “Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials”
Available online 20 January 2023, 113428Author links open overlay panelAndreasSchulte-MecklenbeckaBastianZinnhardtbLouisaM...
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood
Varicella zoster virus (VZV) causes childhood chickenpox, becomes latent in sensory ganglia and reactivates years later to...
Corrigendum to “Humanised mice have functional human neutrophils” [Journal of Immunological Methods, Volume 385, Issues 1–2, 30 November 2012, Pages 96–104]
Volume 513, February 2023, 113423Author links open overlay panelAlice M.CoughlanSimon J.FreeleyMichael G.RobsonPersonEnvel...
Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin
After Clostridium tetani infects the human body, it propagates under anaerobic conditions and produces tetanus neurotoxin ...
Monocyte antigen-presenting capacity to iNKT cells is influenced by the blood collection conditions
It is widely accepted that different blood collection conditions, including anticoagulants, influence leukocyte phenotype ...
Evaluation and optimization of the immunohistochemistry antigen retrieval methods on mouse decalcified joint tissues
To evaluate and optimize antigen retrieval (AR) methods for the detection of NF-κB, interleukin-1β, interleukin-6, interfe...
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy
CD80 or cluster of differentiation 80, also known as B7–1, is a member of the immunoglobulin super family, which binds to ...
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing
Available online 31 December 2022, 113420Author links open overlay panelHighlights•Dried blood spots can readily be used t...
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series
Volume 513, February 2023, 113410Author links open overlay panelHighlights•Antibody immunity from mRNA vaccination can be ...
A method for detection of anti-drug antibodies to a biotherapeutic (CSL112) with endogenous counterpart (apolipoprotein A-I) using a novel sample pre-treatment electrochemiluminescence assay
Available online 29 December 2022, 113411Author links open overlay panelHighlights•A sample pre-treatment electrochemilumi...
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