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Kinetic titration method in flow cytometry for quantification of cell receptors
Kinetic titration method in flow cytometry for quantification of cell receptors
Fluorescence flow cytometry (Robinson and Roederer, 2015; Sklar and Finney, 1982; Stelzer, 1988; Giesecke et al., 2017) is...
Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)
Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)
Black carp (Mylopharyngodon piceus) belongs to the Cyprinidae family and is one of the “four major Chinese carps” in fresh...
An enzyme-free method for isolating testicular macrophages from rodent models
An enzyme-free method for isolating testicular macrophages from rodent models
Macrophages are the major type of immune cell in the testis of both humans and rodents. Testicular macrophages (TMs) play ...
Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists
Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists
Toll-like receptors (TLRs) are type I transmembrane glycoproteins expressed on the membrane of leukocytes, T cells, B cell...
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation
Xenotransplantation—the process of transplanting organs, cells, or tissues from one species to another—has the potential t...
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation
Antibodies (Abs) are essential tools in biomedical research for the detection and quantification of target proteins (Goldm...
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry
The growing incidence of Streptococcus pneumoniae infection (Karcic et al., 2015) highlights the urgent need for vaccines ...
Performance comparison of three commercial tests for the detection of SARS-CoV-2 antibodies in a common set of pediatric samples
Performance comparison of three commercial tests for the detection of SARS-CoV-2 antibodies in a common set of pediatric samples
Serologic diagnosis of COVID-19 is useful for epidemiologic studies and to support diagnosis of patients with post-acute p...
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
Multiple Sclerosis (MS) is an autoimmune disease of the central nervous system that typically manifests in the 2nd, 3rd or...
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
Growth stimulating express gene 2 (ST2) is also known as suppression of tumorigenicity 2, sulfotransferase homolog 2, and ...
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that affects multiple physiological and pathological functions i...
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)
Available online 16 July 2023, 113524Author links open overlay panel, , , , Highlights•New resources for a budding immunol...
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
The latex turbidimetric immunoassay (LTIA), based on antigen-dependent specific aggregation of antibody-immobilized latex ...
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
Lumpy skin disease (LSD) is characterized by fever, enlargement of lymph nodes, anorexia, discharge from the eyes/nose and...
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
Lumpy skin disease (LSD) is characterized by fever, enlargement of lymph nodes, anorexia, discharge from the eyes/nose and...
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells
AIMActivation induced markersCHLAChildren's Hospital Los AngelesCOVID-19Coronavirus disease 2019EDTAEthylenediaminetetraac...
PLGA nanoparticles containing Intimin-Flagellin fusion protein for E. coli O157:H7 nano-vaccine
Hemolytic uremic syndrome (HUS) is the most prevalent and potentially fatal complication associated with Shiga toxin produ...
Impact of delayed PBMC processing on functional and genomic assays
Blood samples are routinely collected from clinical trial participants to improve the understanding of the biology of dise...
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay
Echinococcosis is a unique parasitic disease that is endemic in many countries of the world, particularly in developing co...