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Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing
Despite advances in genetic characterization of Chinese hamster ovary (CHO) cell lines regarding identification of integra...
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system
List of abbreviationsAlBla-7B6-OmBlagene block with alpha beta-lactamase, 7B6 clone and omega beta-lactamaseCDRcomplementa...
Adaptive laboratory evolution to hypersaline conditions, of lactic acid bacteria isolated from seaweed
Seaweed biomass has been proposed as a promising alternative carbon source for fermentation processes using microbial fact...
Comprehensive metabolomics reveals correlation between sophorolipid biosynthesis and autophagy
Sophorolipids are biobased and biodegradable glycolipid surface-active agents contributing to the shift from petroleum to ...
A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase
Available online 20 December 2022Author links open overlay panel, , , Highlights•DyP variant shows increased rates and pro...
AI for Life: Trends in Artificial Intelligence for Biotechnology
Due to popular successes (e.g., ChatGPT) Artificial Intelligence (AI) is on everyone's lips today. When advances in biotec...
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Available online 1 February 2023Author links open overlay panel, , , Highlights•The design-build-test-learn cycle (DBTLc) ...
Synthetic activation of yeast stress response improves secretion of recombinant proteins
Available online 2 January 2023Author links open overlay panelHighlights•Yeasts are powerful hosts for production of singl...
Generation of an anti-idiotypic affibody-based masking domain for conditional activation of EGFR-targeting
Volume 73, 25 March 2023, Pages 9-18Author links open overlay panelHighlights•Development of a specific protein-based mask...
Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds
Available online 10 December 2022Author links open overlay panelHighlights•Nordic microalgae are a promising source of car...
CaRA – A Multi-Purpose Phage Display Library for Selection of Calcium-Regulated Affinity Proteins
Available online 28 November 2022Author links open overlay panelHighlights•Combinatorial library of calcium-regulated affi...
A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform
Volume 72, 25 December 2022, Pages 149-158Author links open overlay panelHighlights•A droplet-based workflow for the genet...
Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay
A homogeneous PCR-based assay for sensitive and specific detection of antibodies in serum or dried blood spots (DBS) is pr...
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5
Volume 72, 25 December 2022, Pages 128-138Author links open overlay panelHighlights•First evidence that D. ferrophilus IS5...
Comparison of process mass intensity (PMI) of continuous and batch manufacturing processes for biologics
Biologics encompasses a wide variety of therapeutics including monoclonal antibodies, fusion proteins, and enzymes, among ...
Controlled Co-immobilisation of Proteins via 4’-Phosphopantetheine-mediated Site-selective Covalent Linkage
Available online 25 October 2022Highlights：•As a fusion tag, ACP enhances soluble expression of target proteins.•ACP can b...
Rheology of microalgae concentrates and its influence on the power consumption of enzymatic hydrolysis processing
The optimization of downstream processing is a critical step in any microalgae-related process. The microalgal biomass is ...
New Vectors for Urea-Inducible Recombinant Protein Production
We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ...
Improving 3-Hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets
3-Hydroxypropionic acid (3-HP) production from renewable feedstocks is of great interest in efforts to develop greener pro...
A standardised methodology for the extraction and quantification of cell-free DNA in cerebrospinal fluid and application to evaluation of Alzheimer’s disease and brain cancers
Cerebrospinal fluid (CSF) is a source of diagnostic biomarkers for a range of neurological conditions. Cell-free DNA (cfDN...
Thauera sp. Sel9, a new bacterial strain for polyhydroxyalkanoates production from volatile fatty acids
Thauera is one of the main genera involved in polyhydroxyalkanoate (PHA) production in microbial mixed cultures (MMCs) fro...
Automatable downstream purification of the benzohydroxamic acid D-DIBOA from a biocatalytic synthesis
Volume 72, 25 December 2022, Pages 48-57Highlights•Design of a 3-steps D-DIBOA purification method without organic solvent...
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies
Available online 20 September 2022Highlights•Soluble and solubilized proteins may show different activities.•The procedure...
A COST Action on microbial responses to low pH: developing links and sharing resources across the academic-industrial divide
Available online 20 September 2022Highlights•Summary of workshop on “Industrial applications of low pH stress on microbial...
Purple bacteria screening for photoautohydrogenotrophic food production: Are new H2-fed isolates faster and nutritionally better than photoheterotrophically obtained reference species?
Available online 29 August 2022HIGHLIGHTS•Ten photoautohydrogenotrophic species were enriched from water treatment microbi...
External Quality Assurance programs for processing methods provide evidence on impact of preanalytical variables
Available online 29 August 2022Highlights•A global analysis of data from almost 1000 processing schemes was performed•The ...
Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids
Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current ...
Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria
Volume 72, 25 December 2022, Pages 1-10Highlights•D-lactic acid production with high optical purity from.•Novel isolates w...
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen
Available online 8 August 2022Highlights•A highly pure recombinant RBD protein (C-RBD-H6 PP) produced in P. pastoris.•Phys...
Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream
Available online 2 August 2022Highlights•Fungal diversity was explored in SBRs aiming at TAG-accumulation from waste fish ...
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