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Automatable downstream purification of the benzohydroxamic acid D-DIBOA from a biocatalytic synthesis
Volume 72, 25 December 2022, Pages 48-57Highlights•Design of a 3-steps D-DIBOA purification method without organic solvent...
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies
Available online 20 September 2022Highlights•Soluble and solubilized proteins may show different activities.•The procedure...
A COST Action on microbial responses to low pH: developing links and sharing resources across the academic-industrial divide
Available online 20 September 2022Highlights•Summary of workshop on “Industrial applications of low pH stress on microbial...
External Quality Assurance programs for processing methods provide evidence on impact of preanalytical variables
Available online 29 August 2022Highlights•A global analysis of data from almost 1000 processing schemes was performed•The ...
Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids
Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current ...
Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria
Volume 72, 25 December 2022, Pages 1-10Highlights•D-lactic acid production with high optical purity from.•Novel isolates w...
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen
Available online 8 August 2022Highlights•A highly pure recombinant RBD protein (C-RBD-H6 PP) produced in P. pastoris.•Phys...
Impact of standardization in tissue processing: The performance of different fixatives
Most tissues in clinical practice are formalin-fixed and paraffin-embedded for histological as well as molecular analyses....
PLASMA PROTEIN BIOMARKER PROFILING REVEALS MAJOR DIFFERENCES BETWEEN ACUTE LEUKAEMIA, LYMPHOMA PATIENTS AND CONTROLS
Aiming to accommodate the unmet need for easily accessible biomarkers with a focus on biological differences between haema...
Derivation of splice junction-specific antibodies using a unique hapten targeting strategy and directed evolution
Alternative splicing of RNA occurs frequently in eukaryotic cells and can result in multiple protein isoforms that are nea...
Iron availability enhances the cellular energetics of aerobic Escherichia coli cultures while upregulating anaerobic respiratory chains
Aerobic Escherichia coli growth at restricted iron concentrations (≤ 1.75 ± 0.04 μM) is characterized by lower biomass yie...
From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes
Volume 70, 25 September 2022, Pages 116-128Highlights•Bacterial citrus microbiomes described using uncultured and cultured...
Closing the loop in bioproduction: Spent Microbial Biomass as a resource within circular bioeconomy
Successful transition to a circular bioeconomy relies on the availability and efficient use of organic feedstocks such as ...
Identification of Lipolytic Enzymes Using High-Throughput Single-cell Screening and Sorting of a Metagenomic Library
The demand for novel, robust microbial biocatalysts for use in industrial and pharmaceutical applications continues to inc...
Poly(3-hydroxybutyrate) production from methane in bubble column bioreactors: Process simulation and design optimization
Volume 70, 25 September 2022, Pages 39-48Highlights•Methane is a valuable substrate for production of PHB.•Improving super...
A new control strategy to improve the mass transfer capacity and reduce air injection costs in raceway reactors
Volume 70, 25 September 2022, Pages 49-56Highlights•Dissolved oxygen control in raceway reactors based on mass transfer de...
Pre-analytical sample stabilization by different sampling devices for PCR-based COVID-19 diagnostics
Available online 8 April 2022Highlights•Elevated temperature has major impact on SARS-CoV-2 RNA stability in 3 of 4 swab s...
Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity
Available online 9 April 2022Highlights•Aspergillus niger possesses four CE16 genes with diverse sequences and expression ...
TILAFeed: a bio-based inventory for circular nutrients management and achieving bioeconomy in future aquaponics
Available online 5 April 2022Highlights•Presented inventory may aid in centralized and ‘one formula’ solutions for aquapon...
A unique biochemical reaction pathway towards trehalulose synthesis by an amylosucrase isolated from Deinococcus deserti
Volume 70, 25 September 2022, Pages 1-8Highlights•A novel amylosucrase was found to synthesize trehalulose as a main produ...