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Profiling of Groundnut bud necrosis orthotospovirus–responsive microRNA and their targets in tomato based on deep sequencing
Profiling of Groundnut bud necrosis orthotospovirus–responsive microRNA and their targets in tomato based on deep sequencing
Tomatoes (Solanum lycopersicum L.) are one of the most widely consumed vegetables in the world and is prized for its flavo...
Insertion of foreign genes into the simian varicella virus genome by Tn7-mediated site-specific transposition
Insertion of foreign genes into the simian varicella virus genome by Tn7-mediated site-specific transposition
Simian varicella virus (SVV, Cercopithecine herpesvirus 9) causes a febrile, vesicular skin rash disease in Old World monk...
A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens
A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens
Volume 327, June 2024, 114939Author links open overlay panel, , , , , , , , Highlights•A sensitive assay developed for use...
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
Emerging infectious diseases are a threat that contributes to the decline of global chelonian species. Herpesviruses are a...
RSV-GenoScan: An automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
RSV-GenoScan: An automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
BackgroundAdvances in high-throughput sequencing (HTS) technologies and reductions in sequencing costs have revolutionised...
Evaluation of an automated platform for the detection of HEV RNA in plasma and stool
Evaluation of an automated platform for the detection of HEV RNA in plasma and stool
Hepatitis E Virus (HEV) is a single-strand positive-sense RNA virus in the family Hepeviridae. Most members of this family...
Improved SARS-CoV-2 RNA recovery in wastewater matrices using a CTAB-based extraction method
Improved SARS-CoV-2 RNA recovery in wastewater matrices using a CTAB-based extraction method
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease 2019 (COVID-19), a...
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening
Senecavirus A (SVA) is the sole member of the genus Senecavirus, belonging to the family Picornaviridae (Adams et al., 201...
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas
Volume 327, June 2024, 114923Author links open overlay panel, , , , , , , , , Highlights•This study describes the developm...
Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)
Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)
Fish gills are anatomically complex tissues that perform a multitude of functions and often comes into direct contact with...
The effect of enzymatic and viability dye treatment in combination with long-range PCR on assessing Tulane virus infectivity
The effect of enzymatic and viability dye treatment in combination with long-range PCR on assessing Tulane virus infectivity
Human norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remain...
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
Bagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and th...
Optimization of duplex digital PCR for the measurement of SARS-CoV-2 RNA
Optimization of duplex digital PCR for the measurement of SARS-CoV-2 RNA
Nucleic acid testing (NAT) is widely adopted for diagnosing infectious diseases and genetic disorders. Through the prolong...
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region
Hepatitis B virus (HBV)-related Hepatocellular carcinoma (HCC) is a prominent form of cancer that ranks among the top mali...
Culturing of SARS-CoV-2 from patient samples: Protocol for optimal virus recovery and assessment of infectious viral load
Culturing of SARS-CoV-2 from patient samples: Protocol for optimal virus recovery and assessment of infectious viral load
Optimal sampling, preservation, and culturing of SARS-CoV-2 from COVID-19 patients are critical for successful recovery of...
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: A study in swine
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: A study in swine
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widesp...
Evaluation of the EasyNAT SARS-CoV-2 assay PCR test for the diagnosis of SARS-CoV-2 infection
Evaluation of the EasyNAT SARS-CoV-2 assay PCR test for the diagnosis of SARS-CoV-2 infection
Reverse transcription polymerase chain reaction (RT-PCR) tests are commonly utilized in commercial settings but pose chall...
Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus
Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus
Lumpy skin disease (LSD) is an acute and sub-acute viral infectious disease of cattle caused by lumpy skin disease virus (...
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
Volume 326, May 2024, 114914Author links open overlay panel, , , , , , , Highlights•Culture independent screening methods ...
An optimised protocol for the expression and purification of adenovirus core protein VII
An optimised protocol for the expression and purification of adenovirus core protein VII
Adenovirus protein VII (pVII) is a highly basic core protein, bearing resemblance to mammalian histones. Despite its diver...
Effective Plant Virus Enrichment Using Carbon Nanotubes and Microfluidics
Effective Plant Virus Enrichment Using Carbon Nanotubes and Microfluidics
Viral diseases of plants cause significant economic losses and are a threat to the world’s food security. Plant virus dete...
Evaluation of the diagnostic assays detecting red sea bream iridovirus infection at different severity levels
Evaluation of the diagnostic assays detecting red sea bream iridovirus infection at different severity levels
Red sea bream iridovirus (RSIV) has been a serious problem in the fish farming industry since 1990 and is the causative ag...
In planta expression of specific single chain fragment antibody (scFv) against nucleocapsid protein of fig mosaic virus (FMV)
In planta expression of specific single chain fragment antibody (scFv) against nucleocapsid protein of fig mosaic virus (FMV)
Ficus carica, commonly known as the common fig, holds great importance as a fruit tree species cultivated in diverse geogr...
The Development of a Rapid, High-Throughput Neutralization Assay Using a SARS-CoV-2 Reporter
The Development of a Rapid, High-Throughput Neutralization Assay Using a SARS-CoV-2 Reporter
Available online 13 February 2024, 114894Author links open overlay panel, , , , , , , , , SummaryMany methods have been de...
Automated Molecular Detection of West Nile Virus in Mosquito Pools using the Panther Fusion System
Automated Molecular Detection of West Nile Virus in Mosquito Pools using the Panther Fusion System
West Nile Virus (WNV) is an arthropod-borne virus spread through mosquito vectors that causes the most domestically acquir...