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Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube
Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube
Volume 322, December 2023, 114812Author links open overlay panel, , , , , , , , , , , Highlights•The assay can detect hMPV...
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus
Duck Tembusu virus disease, caused by duck Tembusu virus (DTMUV), brings great harm to duck industry. Early diagnosis is o...
A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)
A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)
Newcastle disease (ND) caused by virulent avian paramyxovirus type I (APMV-1) is a WOAH and EU listed disease affecting po...
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
Volume 322, December 2023, 114822Author links open overlay panel, , , , , Highlights•A novel multiplex PAstV PCR was devel...
Diagnostic evaluation of Panbio™ antigen rapid diagnostic test for SARS‐CoV‐2: A systematic review and meta-analysis
Diagnostic evaluation of Panbio™ antigen rapid diagnostic test for SARS‐CoV‐2: A systematic review and meta-analysis
IntroductionThe reverse transcriptase polymerase chain reaction (RT-PCR) is the reference diagnostic method for the confir...
Development and validation of an RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
Development and validation of an RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
Epizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has bec...
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody
Peste des petits ruminants virus (PPRV) causes a very devastating disease in sheep and goats. Rapid diagnosis and immunisa...
Establishment of two visual interpretation methods of DIV1 LAMP amplification products
Establishment of two visual interpretation methods of DIV1 LAMP amplification products
Viral diseases in shrimp have become a significant impediment to the sustainable development of the global shrimp aquacult...
A rapid and affordable amplicon-based method for next-generation genome sequencing of the infectious bursal disease virus
A rapid and affordable amplicon-based method for next-generation genome sequencing of the infectious bursal disease virus
Available online 6 September 2023, 114807Author links open overlay panel, , , , , , , , , , Highlights•A Multiplex-PCR-NGS...
Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration
Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration
Zucchini yellow mosaic virus (ZYMV) infects cucurbits causing yellow mosaic in leaves, malformations in fruits, and degrad...
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
Available online 27 August 2023, 114804Author links open overlay panel, , , , , Highlights•A one-step duplex RT-qPCR assay...
Corrigendum to “Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections” J. Virol. Methods 206 (2014) 38–41
Corrigendum to “Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections” J. Virol. Methods 206 (2014) 38–41
Volume 321, November 2023, 114803Author links open overlay panelAlonzo Alfaro-Núñez, M. Thomas P. GilbertShow morePrevious...
Optimizing Virus Inactivation Methods for Molecular Detection Techniques: Implications for Viral Protein and RNA Measurements
Optimizing Virus Inactivation Methods for Molecular Detection Techniques: Implications for Viral Protein and RNA Measurements
Available online 23 August 2023, 114801Author links open overlay panel, , , Highlights•The virus inactivation methods were...
Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification
Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification
Available online 24 August 2023, 114802Author links open overlay panel, , , , , , , , , Highlights•Quantification of mpox ...
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples.
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples.
Non-targeted next generation sequencing (NGS) is widely applied to identify the diversity of pathogens in field samples. H...
Validation of a TaqMan one-step real-time RT-PCR assay targeting ISAV segment 7 spliced mRNA
Validation of a TaqMan one-step real-time RT-PCR assay targeting ISAV segment 7 spliced mRNA
Available online 8 August 2023, 114791Author links open overlay panel, , , , , , Highlights•Validation of TaqMan one-step ...
Successful Transduction of Target Gene Mediated by Adeno-Associated Virus 2 into Lens Epithelial Cells in Rats
Successful Transduction of Target Gene Mediated by Adeno-Associated Virus 2 into Lens Epithelial Cells in Rats
The Adeno-Associated Virus (AAV) has emerged as a promising candidate for delivery of genetic material, exhibiting signifi...
Comparing solid-based concentration methods for rapid and efficient recovery of SARS-CoV-2 for wastewater surveillance
Comparing solid-based concentration methods for rapid and efficient recovery of SARS-CoV-2 for wastewater surveillance
As wastewater-based surveillance of SARS-CoV-2 attracts interest globally, there is a need to evaluate and identify rapid ...
ConCreT, a 2D convolutional neural network for taxonomic classification applied to viruses in the phylum Cressdnaviricota
Available online 2 August 2023, 114789Author links open overlay panel, AbstractTaxonomic assignments allow scientists to c...
AN INDIRECT ELISA TO DETECT ANTIBODIES TO THE gC OF BOVINE ALPHAHERPESVIRUS 1 (BoAHV1) DISPLAYING NO CROSSREACTIVITY WITH ANTIBODIES INDUCED BY BOVINE ALPHAHERPESVIRUS 5 (BoAHV5)
Seroprevalence of bovine alphaherpesvirus type 1 (BoAHV1) infections may be contaminated by crossreactive antibodies to bo...
Low BAU/ml values with 4 of 5 SARS CoV-2 spike-specific monoclonal antibodies in the Roche Elecsys antibody assay
BackgroundThe Abbott SARS-CoV-2 IgG Quant II assay and the Roche Elecsys double antigen sandwich (DAgS) immunoassay measur...
Rapid and Sensitive Single-Sample Viral Metagenomics using Nanopore Flongle Sequencing
Available online 28 July 2023, 114784Author links open overlay panel, , , , , , Highlights•Development of a viral metageno...
Development of the isothermal recombinase polymerase amplification assays for rapid detection of the genus Capripoxvirus
Available online 28 July 2023, 114788Author links open overlay panel, , , , , , , Highlights•Isothermal real-time RPA and ...
Single-domain antibodies applied as antiviral immunotherapeutics
Viral infections have been the cause of high mortality rates throughout different periods in history. Over the last two de...
Subtype specific virus enrichment with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus
Wild bird avian influenza type A virus (AIV) surveillance is important for the early detection of highly pathogenic AIVs a...
Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method
Available online 22 July 2023, 114775Author links open overlay panel, , , , , , , Highlights•This method is an indirect EL...
Designing of Rapid Assay for the Detection of RdRp/Orf1ab Specific to SARS-CoV-2
Available online 17 July 2023, 114774Author links open overlay panel, , , , Highlights•RdRp/Orf1ab was detected by develop...
Subtype specific virus enrichement with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus
Wild bird avian influenza type A virus (AIV) surveillance is important for the early detection of highly pathogenic AIVs a...
Examination of the cross-reactivity between vaccinia virus Tiantan strain and monkeypox virus
AimTo investigate the cross-reactivity between the sera collected from Vaccinia Virus Tiantan Strain vaccinated rabbits an...
A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation
Virus-like particles (VLPs) is one of the most favourable subjects of study, especially in the field of nanobiotechnology ...
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