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Vacuoles isolated from Saccharomyces cerevisiae inhibit differentiation of 3T3-L1 adipocyte
Yeast vacuoles contain various hydrolytic enzymes including lipase. They play important roles in intracellular signaling a...
Biochemical characterization of the β-glucosidase Glu1B from Coptotermes formosanus produced in Pichia pastoris
β-glucosidases (E.C. 3.2.1.21) are enzymes that hydrolyze β-1,4-glycosidic bonds from non-reducing terminal residues in β-...
Regiospecific 3′-C-prenylation of naringenin by Nocardiopsis gilva prenyltransferase
The prenylation of flavonoids is a main type of structural modification and can endow flavonoids with greater bioactivity ...
Development of a compartmentalised self-replication protocol for selection of superior blunt-end DNA ligases
DNA ligases are widely used in molecular biology to generate recombinant DNA. However, having evolved for nick-sealing, th...
Biochemical and electrochemical characterization of biofilms formed on everolimus-eluting coronary stents
Drug-eluting stents (DES) are mostly used in percutaneous coronary intervention, which is the main treatment for coronary ...
De novo biosynthesis of N-acetyltyramine in engineered Escherichia coli
N-acetyltyramine as a tyramine alkaloid has drawn great attention because of its excellent anti-free radical, antithrombot...
Enantioselective bio-deacylation of arylalkyl acetates using tertiary amines as additive under promiscuous conditions
Herein, we describe the impact of the introduction of tertiary amines as additive during the enzymatic kinetic resolution ...
Engineering the filamentous fungus Penicillium oxalicum for rapid, low-background and efficient protein expression
Filamentous fungi are widely used in the field of recombinant protein expression due to their well-established protein mod...
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)
Enzymatic degradation of poly(ethylene terephthalate) (PET) has emerged as a promising route for ecofriendly biodegradatio...
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor
Fish consumption is essential for a healthy diet. However, all seafood including fish are susceptible to deterioration unl...
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface
A plasmid was constructed to express the CapA-SpeA fusion protein from the capA gene, which encodes one of the subunits of...
Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase
Milk glycoproteins play various biological roles including antibacterial, antiviral activities, modulating immune response...
Mutation of conserved residues in the laminarinase Lam1092 increases the antioxidant activity of the laminarin product hydrolysates
Volume 162, January 2023, 110135Highlights•Laminarinases of GH16 were only present in the GH16_3 subfamily based on phylog...
The strategy of cell extract based metal organic frameworks (CE-MOF) for improved enzyme characteristics
A convenient cell extract based metal organic frameworks (CE-MOF) strategy was used to produce self-assembled hybrid micro...
Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25
Recent advancements in biorefinery processes necessitate search for cost effective and thermostable cellulases. This study...
Enzymatic biosensor based on dendritic gold nanostructure and enzyme precipitation coating for glucose sensing and detection
With the development of blood glucose monitoring medical devices, higher requirements are put forward for the sensitivity ...
Rational engineering of a metalloprotease to enhance thermostability and activity
The rational design of enzymes with enhanced thermostability is efficient. Solvent-tolerant metalloprotease from Pseudomon...
Improving the catalytic efficiency of Pseudomonas aeruginosa lipoxygenase by semi-rational design
Available online 5 September 2022, 110120Highlights•Potential activity-enhancing mutation sites on the lid structure were ...
A mutant of Monascus purpureus obtained by carbon ion beam irradiation yielded yellow pigments using various nitrogen sources
Available online 6 September 2022, 110121Highlights•Mutant of Monascus with high-yielding MYPs and citrinin-free productio...