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Non-covalent binding tags for batch and flow biocatalysis
Enzymes are often immobilized to allow their recovery and reuse from batch reactors or so that they can be used as heterog...
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose
In recent years, rare sugars have gained attention as a result of their numerous applications. According to the Internatio...
A study of targeted mutation of L-rhamnose isomerase to improve the conversion efficiency of D-allose
In recent years, rare sugars have gained attention as a result of their numerous applications. According to the Internatio...
Microbial engineering of Methylorubrum extorquens AM1 to enhance CO2 conversion into formate
Methylorubrum extorquens AM1 is an alpha-proteobacteria that can utilize C1 compounds (e.g., methanol [1], formate [2], an...
Engineering acyl-ACP reductase with fusion tags enhances alka(e)ne synthesis in Escherichia coli
Current energy sources are facing increasing challenges related to long-term sustainability because of climate changes and...
THERMOPLASTIC STARCH (TPS) BIOPLASTIC, THE GREEN SOLUTION FOR SINGLE-USE PETROLEUM PLASTIC FOOD PACKAGING – A REVIEW
Plastics have become one of the backbones of the global economy and have grown to be one of the world's largest material c...
Alternative Secretory Signal Sequences for Recombinant Protein Production in Pichia pastoris
Yeasts are prominent expression systems for recombinant protein production due to the advantages they offer in basic resea...
Thr22 plays an important role in the efficient catalytic process of Bacillus subtilis chitosanase BsCsn46A
Chitosan is composing of glucosamine monomers linked by β-1, 4-glycosidic bonds[1], [2]. Chitooligosaccharides (COS) are h...
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade
Naringenin is a kind of flavonoid with many kinds of pharmacological activities, and is also a key intermediate metabolite...
Production of 2’-Fucosyllactose using ⍺1,2-fucosyltransferase from a GRAS bacterial strain
2'-Fucosyllactose (2’-FL) is a major oligosaccharide found in human breast milk. It is produced from GDP-L-fucose and D-la...
High-level itaconic acid (IA) production using engineered Escherichia coli Lemo21(DE3) toward sustainable biorefinery
Itaconic acid (IA) serves as a prominent building block for polyamides as sustainable material. In vivo IA production is f...
Xylose isomerase from Piromyces sp. E2 is a promiscuous enzyme with epimerase activity
Xylose isomerase catalyzes the isomerization of D-xylose to D-xylulose with promiscuous activity for other saccharides inc...
A Novel NADH-dependent Leucine Dehydrogenase for Multi-step Cascade Synthesis of L-phosphinothricin
L-Phosphinothricin (L-PPT) is the effective constituent in racemic PPT (a high-efficiency and broad-spectrum herbicide), a...
Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw
Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars thro...
One-pot biosynthesis of gastrodin using UDP-glycosyltransferase itUGT2 with an in situ UDP-glucose recycling system
Gastrodin, the major effective ingredient in Tianma (Gastrodia elata), is a p-hydroxybenzoic acid derivative with various a...