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Non-covalent binding tags for batch and flow biocatalysis
Enzymes are often immobilized to allow their recovery and reuse from batch reactors or so that they can be used as heterog...
Promoter engineering for efficient production of sucrose phosphorylase in Bacillus subtilis and its application in enzymatic synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid
L-ascorbic acid (L-AA) is known as vitamin C and is one of the essential nutrients. It displays various physiological acti...
Application of reductive amination by heterologously expressed Thermomicrobium roseum L-alanine dehydrogenase to synthesize L-alanine derivatives
Unnatural amino acids (UAA) play an important role in the production of pharmaceuticals, with an increasingly large pool u...
Targeted gene manipulation of Leloir pathway genes for the constitutive expression of β-galactosidase and its transgalactosylation product galacto-oligosaccharides from Kluyveromyces lactis GG799 and knockout strains
Kluyveromyces lactis is one of few yeast species that can utilize lactose or galactose as a sole source of carbon and ener...
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose
In recent years, rare sugars have gained attention as a result of their numerous applications. According to the Internatio...
Application of Reductive Amination by Heterologously Expressed Thermomicrobium roseum L-alanine Dehydrogenase to Synthesize L-Alanine Derivatives
Unnatural amino acids (UAA) play an important role in the production of pharmaceuticals, with an increasingly large pool u...
A study of targeted mutation of L-rhamnose isomerase to improve the conversion efficiency of D-allose
In recent years, rare sugars have gained attention as a result of their numerous applications. According to the Internatio...
Microbial engineering of Methylorubrum extorquens AM1 to enhance CO2 conversion into formate
Methylorubrum extorquens AM1 is an alpha-proteobacteria that can utilize C1 compounds (e.g., methanol [1], formate [2], an...
Engineering acyl-ACP reductase with fusion tags enhances alka(e)ne synthesis in Escherichia coli
Current energy sources are facing increasing challenges related to long-term sustainability because of climate changes and...
Genipin and glutaraldehyde based laccase two-layers immobilization with improved properties: new biocatalysts with high potential for enzymatic removal of trace organic contaminants
Current research carried out on bioprocess engineering topics has focused heavily on the design of green and environmental...
THERMOPLASTIC STARCH (TPS) BIOPLASTIC, THE GREEN SOLUTION FOR SINGLE-USE PETROLEUM PLASTIC FOOD PACKAGING – A REVIEW
Plastics have become one of the backbones of the global economy and have grown to be one of the world's largest material c...
Continuous production of gamma aminobutyric acid by engineered and immobilized Escherichia coli whole-cells in a small-scale reactor system
Gamma aminobutyric acid (GABA) is a four-carbon non-proteinogenic amino acid synthesized in plants, fungi, vertebrates, an...
Alternative Secretory Signal Sequences for Recombinant Protein Production in Pichia pastoris
Yeasts are prominent expression systems for recombinant protein production due to the advantages they offer in basic resea...
Highly selective preparation of N-terminus Horseradish peroxidase-DNA conjugate with fully retained enzymatic activity: HRP-DNA structure – activity relation
Covalent protein–oligonucleotide (POC) conjugates (POC) are intensely studied for a broad range of applications in biology...
Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability
The growth of societal demand has exacerbated the global shortage of resources and energy, especially the depletion of oil...
Heterologous expression, purification, and characterization of a recombinant Cordyceps militaris lipase from Candida rugosa-like family in Pichia pastoris
Lipases (EC 3.1.1.3) are serine hydrolases that catalyze the hydrolysis and esterification of various water-insoluble subs...
Enhancement of biohydrogen production in Clostridium acetobutylicum ATCC 824 by overexpression of glyceraldehyde-3-phosphate dehydrogenase gene
The excessive use of fossil fuels accelerated global warming; however, most of the energy is still dependent on fossil fue...
ZIF-8 as support for enhanced stability of immobilized lipase used with a thermoresponsive switchable solvent to simplify the microalgae-to-biodiesel process
Global fossil fuel consumption has increased over the years. An increase in fossil fuel consumption by 5.8% in 2021 was re...
Thr22 plays an important role in the efficient catalytic process of Bacillus subtilis chitosanase BsCsn46A
Chitosan is composing of glucosamine monomers linked by β-1, 4-glycosidic bonds[1], [2]. Chitooligosaccharides (COS) are h...
Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog
γ-Glutamyl transpeptidase (GGT; EC 2.3.2.2) is an enzyme that is widely distributed in nature and is known to catalyze the...
Simultaneous enhancement of thermostability and catalytic activity of κ-carrageenase from Pseudoalteromonas tetraodonis by rational design
κ-Carrageenan is a linear polysaccharide with repeating disaccharide units of 3,6-anhydro-α-D-galactose and β-D-galactose-...
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade
Naringenin is a kind of flavonoid with many kinds of pharmacological activities, and is also a key intermediate metabolite...
Production of 2’-Fucosyllactose using ⍺1,2-fucosyltransferase from a GRAS bacterial strain
2'-Fucosyllactose (2’-FL) is a major oligosaccharide found in human breast milk. It is produced from GDP-L-fucose and D-la...
High-level itaconic acid (IA) production using engineered Escherichia coli Lemo21(DE3) toward sustainable biorefinery
Itaconic acid (IA) serves as a prominent building block for polyamides as sustainable material. In vivo IA production is f...
Long-term exposure of polytetrafluoroethylene-nanoplastics on the nitrogen removal and extracellular polymeric substances in sequencing batch reactor
The impact of polytetrafluoroethylene-nanoplastics (PTFE-NPs) on biological sewage disposal was delved, containing nitroge...
Xylose isomerase from Piromyces sp. E2 is a promiscuous enzyme with epimerase activity
Xylose isomerase catalyzes the isomerization of D-xylose to D-xylulose with promiscuous activity for other saccharides inc...
Enantioconvergent hydrolysis of racemic 1,2-epoxypentane and 1,2-epoxyhexane by an engineered Escherichia coli strain overexpressing a novel Streptomyces fradiae epoxide hydrolase
In order to excavate microbial epoxide hydrolases (EHs) with desired catalytic properties, a novel EH, SfEH1, was identifi...
A Novel NADH-dependent Leucine Dehydrogenase for Multi-step Cascade Synthesis of L-phosphinothricin
L-Phosphinothricin (L-PPT) is the effective constituent in racemic PPT (a high-efficiency and broad-spectrum herbicide), a...
Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw
Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars thro...
One-pot biosynthesis of gastrodin using UDP-glycosyltransferase itUGT2 with an in situ UDP-glucose recycling system
Gastrodin, the major effective ingredient in Tianma (Gastrodia elata), is a p-hydroxybenzoic acid derivative with various a...
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