Chemicals, cell lines, and culture conditions

Commercial kits

Preparation of TQ, silymarin and cisplatin working solutions

Antiproliferative assay

Calculation of inhibitory concentration (IC₅₀)

Calculation of combination index (CI)

Calculation of resistance fold

Colorimetric assay of caspase-3 activity in EMT-6/P and EMT-6/CPR cells ()

Mice

Establishing regimens for the in vivo experiment

Tumor inoculation and antitumor activity in vivo

Evaluation of liver function in treated mice

Evaluation of kidney function in treated mice

Data analysis