Materials

Construction of pseudoternary phase diagram

Preparation of microemulsion

Measurement of particle size, PDI and zeta potential

Visual inspection and centrifugation test

Dilution test

EC test

Physical stability tests

pH measurement

Content uniformity test

In vitro drug release

Preparation of ME-based hydrogel

Visual appearance

pH measurement

Spreadability test

Viscosity and rheology studies

Ex vivo permeability study

Ex vivo skin deposition study

Statistical analysis