Study of the antitumor activity of the combination baicalin and epigallocatechin gallate in a murine model of vincristine-resistant breast cancer

Chemicals, cell lines and culture conditions

Commercial kits

Preparation of epigallocatechin Gallate, baicalin, and vincristine working solutions

Antiproliferative assay

Calculation of inhibitory concentration (IC₅₀)

Calculation of resistance fold

Colorimetric assay of caspase-3 activity in EMT-6/V cells

Mice

Establishing regimens for the in vivo experiment

Tumor inoculation and antitumor activity in vivo

Evaluation of liver function in treated mice

Evaluation of kidney function in treated mice

Data analysis

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