Preparation of the extract and preliminary analysis

Determination of DPPH radical scavenging activity

Determination of the scavenging of superoxide radicals by alkaline DMSO

Anti-inflammatory activity by inhibition of albumin denaturation

Active substance screening and target prediction

NAFLD-related target screening

Potential target acquisition of HAEPN for NAFLD therapy

Establishing a protein-protein interaction network and identifying key targets

Functional enrichment in gene ontology (GO) and pathway analysis in the Kyoto Encyclopaedia of Genes and Genomes (KEGG)

Architecture of the compound-target pathway network

Molecular docking

The pedagogy of the concoction of macromolecules

Preparation of the ligand file

The construction of mating pockets

Docking and visualization