Advances in Pharmacology and Pharmacy Vol. 12(4), pp. 380 - 391
DOI: 10.13189/app.2024.120410
Reprint (PDF) (911Kb)

Chetan T. Bhat *, Panchaxari M. Dandagi , Pradnya G. Patted
Department of Pharmaceutics, KLE College of Pharmacy, Constituent Unit of KLE Academy of Higher Education and Research, Belagavi, Karnataka, India

ABSTRACT

Aim: This study aimed to develop a safe and effective green solvent-assisted UV-visible spectrophotometric method and validation of an established method for estimating methotrexate in different dosage forms. Method: The solubility profile, melting point, and Fourier Transform Infra-Red were used to characterize the purity of methotrexate (MTX). The drug MTX was analyzed using the UV-visible spectrophotometric method using 1% Sodium Lauryl Sulfate solution in Phosphate buffer saline solution pH 6.8 as a green solvent. The method was validated by using different parameters like linearity, accuracy, precision, specificity, the limit of detection, the limit of quantitation, robustness and ruggedness, and forced degradation studies. The assay of marketed formulation and nano transethosomes was carried out using an established green solvent method. Result: The melting point of pure methotrexate was found 195℃, which nearly corresponds to its actual melting range. FTIR graph by showing sharp characteristic peaks confirmed the purity of the MTX by the presence of functional groups. The wavelength corresponding to the maximum absorbance of the drug was found at 303 nm. The linear range was found with the absorbance of Y = 0.064x + 0.0116 and regression coefficient R2 = 0.9992 for the concentration range of 2-14 µg/ml of MTX. It was discovered that the repeatability, accuracy, and precision for both intra-day and inter-day were within the acceptable ranges. The limit of detection (LOD) and limit of quantitation (LOQ), which were found to be 0.0825 µg/mL and 0.25 µg/mL, respectively, were determined in order to establish the sensitivity of the technique. Conclusions: The drug MTX was confirmed by the interpretation of UV spectra. This technique may be used for regular analysis of MTX in various pharmaceutical dosage forms and was shown to be easy to use, inexpensive, repeatable, and friendly to the environment.

KEYWORDS
Methotrexate, Phosphate Buffer Solution pH 6.8, Spectrophotometric Method, Sodium Lauryl Sulfate, Green Solvent, Validation, Beer's Law

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Chetan T. Bhat , Panchaxari M. Dandagi , Pradnya G. Patted , "Environmental Friendly Analysis: Development and Validation of UV-Visible Spectrophotometric Method for Methotrexate Quantification in Marketed Dosage Forms and Nano Transethosomes," Advances in Pharmacology and Pharmacy, Vol. 12, No. 4, pp. 380 - 391, 2024. DOI: 10.13189/app.2024.120410.

(b). APA Format:
Chetan T. Bhat , Panchaxari M. Dandagi , Pradnya G. Patted (2024). Environmental Friendly Analysis: Development and Validation of UV-Visible Spectrophotometric Method for Methotrexate Quantification in Marketed Dosage Forms and Nano Transethosomes. Advances in Pharmacology and Pharmacy, 12(4), 380 - 391. DOI: 10.13189/app.2024.120410.