Advances in Pharmacology and Pharmacy Vol. 12(4), pp. 289 - 297
DOI: 10.13189/app.2024.120402
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Cordelia Mano John , Rajamiriyam M , Jonnalagadda Bhavana , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy *
Department of Biomedical Sciences, Sri Ramachandra Institute of Higher Education and Research, India

ABSTRACT

The immunosuppressive agent tacrolimus (TAC), a calcineurin inhibitor (CNI), is a crucial component in solid organ transplantation, contributing to renal damage. CNI-associated nephrotoxicity is majorly manifested in the proximal tubules of the kidney and plays a central role in oxidative stress. As oxidative stress is a contributing factor in nephrotoxicity, the effective antioxidant activity of sinapic acid (SIA; 3,5-dimethoxy-a 4-hydroxycinnamic acid) could help as a novel nephroprotective antioxidant. Studies have shown that polyphenols like SIA exhibit robust antioxidant effects by scavenging ROS, RNS, and mitigating oxidative stress. In this experimental study, the impact of SIA against TAC-induced nephrotoxicity was evaluated in MDCK by measuring cell viability (MTT assay), oxidative stress (ROS), and apoptosis (AO/EB staining). Additionally, levels of lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and cell cycle progression were examined. SIA demonstrated the ability to avert TAC-induced cell death and DNA damage. Statistical analysis employing unpaired t-Test revealed a significant reversal of cell cycle arrest, reduced cell population in the sub G0/G1 phase, and improved levels of GSH and SOD along with decreased LPO levels with SIA co-treatment. In conclusion, SIA was proved effective in preventing TAC-induced nephrotoxic alterations in MDCK cells.

KEYWORDS
Sinapic Acid, Tacrolimus, Oxidative Stress, GSH, SOD, Cell Cycle Analysis

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Cordelia Mano John , Rajamiriyam M , Jonnalagadda Bhavana , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy , "Sinapic Acid (3,5-dimethoxy-4-hydroxycinnamic Acid) as Potential Protective Agent Against Tacrolimus Induced Nephrotoxicity in MDCK Cells," Advances in Pharmacology and Pharmacy, Vol. 12, No. 4, pp. 289 - 297, 2024. DOI: 10.13189/app.2024.120402.

(b). APA Format:
Cordelia Mano John , Rajamiriyam M , Jonnalagadda Bhavana , Yadav Sangeeta Muthyalaiah , Sumathy Arockiasamy (2024). Sinapic Acid (3,5-dimethoxy-4-hydroxycinnamic Acid) as Potential Protective Agent Against Tacrolimus Induced Nephrotoxicity in MDCK Cells. Advances in Pharmacology and Pharmacy, 12(4), 289 - 297. DOI: 10.13189/app.2024.120402.