Advances in Pharmacology and Pharmacy Vol. 12(3), pp. 216 - 227
DOI: 10.13189/app.2024.120306
Reprint (PDF) (776Kb)

Dhara Parekh 1,*, Vijay Lambole 2, Vipul Gajera 1, Tanvi Desai 1
1 Department of Pharmacology, Shree Naranjibhai Lalbhai Patel College of Pharmacy, Umrakh, Gujarat, India
2 Department of Pharmacology, Sir Dr. M. S. Gosavi College of Pharmaceutical Education and Research, Nashik, Maharashtra, India

ABSTRACT

Background: Perilla Frutescens (PF) has traditionally been used in depression-related diseases, as a sedative and anti-oxidant agent. Objective: This study intended to appraise the anxiolytic effects of PF on experimental animals using n-hexane extract (NHE) and ethanolic extract (EE) of PF. Methods: The animals were divided into six separate groups, with each group comprising 6 Swiss albino mice of either sex. PF seeds powder is extracted with NHE, and EE and given orally at a dosage ranging from 1-2 ml/kg of body weight. To evaluate anxiolytic behavior, we employed several tests, including the Elevated Plus Maze (EPM), light-dark exploration test (LDE), open-field test (OFT), Photoactometer (PA), and hole-board test (HBT). Additionally, we assessed various biochemical parameters such as superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). Result: In the EPM model, both extracts exhibited a significant rise in the percentage of time spent and the number of entries into the open arm. The LDE test demonstrated a prolonged duration of entry into the light box and an increased period spent in the light compartments. Moreover, in the OFT, there was a notable rise in the number of squares crossed. Locomotor activity is measured with a PA, and lower cut-off numbers distinguish extract-treated groups from diazepam-treated normal control groups. The HBT demonstrated a dose-dependent increase in the number of head dips. Conclusion: Both extracts exhibited notable anxiolytic activity, but the most favorable outcome was achieved by administering a 2 ml/kg dose of NHE of PF in all the models. The present research suggests that chemical constituents of PF are an acceptable candidate for the prophylaxis of AD.

KEYWORDS
Perilla frutescens, Anxiolytics, Anxiety, Elevated Plus Maze, Light-Dark Exploration Test, Open-Field Test, Photoactometer, Hole-Board Test

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Dhara Parekh , Vijay Lambole , Vipul Gajera , Tanvi Desai , "Evaluation of Anti-Anxiety Effect of Perilla Frutescens Seed Extracts on Experimental Animals," Advances in Pharmacology and Pharmacy, Vol. 12, No. 3, pp. 216 - 227, 2024. DOI: 10.13189/app.2024.120306.

(b). APA Format:
Dhara Parekh , Vijay Lambole , Vipul Gajera , Tanvi Desai (2024). Evaluation of Anti-Anxiety Effect of Perilla Frutescens Seed Extracts on Experimental Animals. Advances in Pharmacology and Pharmacy, 12(3), 216 - 227. DOI: 10.13189/app.2024.120306.