β-lapachone-mediated WST1 Reduction as Indicator for the Cytosolic Redox Metabolism of Cultured Primary Astrocytes

Gong Q, Hu J, Wang P, Li X, Zhang X (2021) A comprehensive review on β-lapachone: mechanisms, structural modifications, and therapeutic potentials. Eur J Med Chem 210:112962. https://doi.org/10.1016/j.ejmech.2020.112962

Article  CAS  PubMed  Google Scholar 

Gomes CL, de Albuquerque Wanderley Sales V, Gomes de Melo C, Ferreira da Silva RM, Vicente Nishimura RH, Rolim LA, Rolim Neto PJ (2021) Beta-lapachone: natural occurrence, physicochemical properties, biological activities, toxicity and synthesis. Phytochemistry 186:112713. https://doi.org/10.1016/j.phytochem.2021.112713

Article  CAS  PubMed  Google Scholar 

Preethi S, Arthiga K, Patil AB, Spandana A, Jain V (2022) Review on NAD(P)H dehydrogenase quinone 1 (NQO1) pathway. Mol Biol Rep 49:8907–8924. https://doi.org/10.1007/s11033-022-07369-2

Article  CAS  PubMed  Google Scholar 

Ross D, Siegel D (2021) The diverse functionality of NQO1 and its roles in redox control. Redox Biol 41:101950. https://doi.org/10.1016/j.redox.2021.101950

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang K, Chen D, Ma K, Wu X, Hao H, Jiang S (2018) NAD(P)H:Quinone Oxidoreductase 1 (NQO1) as a therapeutic and diagnostic target in Cancer. J Med Chem 61:6983–7003. https://doi.org/10.1021/acs.jmedchem.8b00124

Article  CAS  PubMed  Google Scholar 

Ehrke E, Steinmeier J, Stapelfeldt K, Dringen R (2021) The menadione-mediated WST1 reduction by cultured astrocytes depends on NQO1 activity and cytosolic glucose metabolism. Neurochem Res 46:88–99. https://doi.org/10.1007/s11064-019-02930-1

Article  CAS  PubMed  Google Scholar 

Steinmeier J, Kube S, Karger G, Ehrke E, Dringen R (2020) β-Lapachone induces acute oxidative stress in rat primary astrocyte cultures that is terminated by the NQO1-inhibitor dicoumarol. Neurochem Res 45:2442–2455. https://doi.org/10.1007/s11064-020-03104-0

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hart CG, Karimi-Abdolrezaee S (2021) Recent insights on astrocyte mechanisms in CNS homeostasis, pathology, and repair. J Neurosci Res 99:2427–2462. https://doi.org/10.1002/jnr.24922

Article  CAS  PubMed  Google Scholar 

Dringen R, Brandmann M, Hohnholt MC, Blumrich E-M (2015) Glutathione-dependent detoxification processes in astrocytes. Neurochem Res 40:2570–2582. https://doi.org/10.1007/s11064-014-1481-1

Article  CAS  PubMed  Google Scholar 

Bonvento G, Bolaños JP (2021) Astrocyte-neuron metabolic cooperation shapes brain activity. Cell Metabol 33:1546–1564. https://doi.org/10.1016/j.cmet.2021.07.006

Article  CAS  Google Scholar 

Beard E, Lengacher S, Dias S, Magistretti PJ, Finsterwald C (2022) Astrocytes as key regulators of brain energy metabolism: new therapeutic perspectives. Front Physiol 12:825816. https://doi.org/10.3389/fphys.2021.825816

Article  PubMed  PubMed Central  Google Scholar 

Jiwaji Z, Hardingham GE (2022) Good, bad, and neglectful: astrocyte changes in neurodegenerative disease. Free Radic Biol Med 182:93–99. https://doi.org/10.1016/j.freeradbiomed.2022.02.020

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bohmbach K, Henneberger C, Hirrlinger J (2022) Astrocytes in memory formation and maintenance. Essays Biochem. https://doi.org/10.1042/ebc20220091

Article  Google Scholar 

Park J-S, Lee Y-Y, Kim J, Seo H, Kim H-S (2016) β-Lapachone increases phase II antioxidant enzyme expression via NQO1-AMPK/PI3K-Nrf2/ARE signaling in rat primary astrocytes. Free Radic Biol Med 97:168–178. https://doi.org/10.1016/j.freeradbiomed.2016.05.024

Article  CAS  PubMed  Google Scholar 

Kim AY, Jeong K-H, Lee JH, Kang Y, Lee SH, Baik EJ (2017) Glutamate dehydrogenase as a neuroprotective target against brain ischemia and reperfusion. Neuroscience 340:487–500. https://doi.org/10.1016/j.neuroscience.2016.11.007

Article  CAS  PubMed  Google Scholar 

Dinkova-Kostova AT, Talalay P (2010) NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector. Arch Biochem Biophys 501:116–123. https://doi.org/10.1016/j.abb.2010.03.019

Article  CAS  PubMed  PubMed Central  Google Scholar 

Stapelfeldt K, Ehrke E, Steinmeier J, Rastedt W, Dringen R (2017) Menadione-mediated WST1 reduction assay for the determination of metabolic activity of cultured neural cells. Anal Biochem 538:42–52. https://doi.org/10.1016/j.ab.2017.09.011

Article  CAS  PubMed  Google Scholar 

Raabe J, Arend C, Steinmeier J, Dringen R (2019) Dicoumarol inhibits multidrug resistance protein 1-mediated export processes in cultured primary rat astrocytes. Neurochem Res 44:333–346. https://doi.org/10.1007/s11064-018-2680-y

Article  CAS  PubMed  Google Scholar 

Steinmeier J, Dringen R (2019) Exposure of cultured astrocytes to menadione triggers rapid radical formation, glutathione oxidation and Mrp1-mediated export of glutathione disulfide. Neurochem Res 44:1167–1181. https://doi.org/10.1007/s11064-019-02760-1

Article  CAS  PubMed  Google Scholar 

Dringen R, Hoepken HH, Minich T, Ruedig C (2007) Pentose phosphate pathway and NADPH metabolism. In: Lajtha A, Gibson GE, Dienel GA (eds) Handbook of neurochemistry and molecular neurobiology: brain energetics integration of molecular and cellular processes. Springer, Heidelberg, pp 41–62

Chapter  Google Scholar 

Liddell JR, Zwingmann C, Schmidt MM, Thiessen A, Leibfritz D, Robinson SR, Dringen R (2009) Sustained hydrogen peroxide stress decreases lactate production by cultured astrocytes. J Neurosci Res 87:2696–2708. https://doi.org/10.1002/jnr.22093

Article  CAS  PubMed  Google Scholar 

Tulpule K, Hohnholt MC, Hirrlinger J, Dringen R (2014) Primary cultures of astrocytes and neurons as model systems to study the metabolism and metabolite export from brain cells. In: Hirrlinger J, Waagepetersen HS (eds) Brain Energy Metabolism. Springer, New York, pp 45–72

Chapter  Google Scholar 

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275. https://doi.org/10.1016/S0021-9258(19)52451-6

Article  CAS  PubMed  Google Scholar 

Tietze F (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522. https://doi.org/10.1016/0003-2697(69)90064-5

Article  CAS  PubMed  Google Scholar 

Heinz S, Freyberger A, Lawrenz B, Schladt L, Schmuck G, Ellinger-Ziegelbauer H (2017) Mechanistic investigations of the mitochondrial complex I inhibitor rotenone in the context of pharmacological and safety evaluation. Sci Rep 7:45465. https://doi.org/10.1038/srep45465

Article  CAS  PubMed  PubMed Central  Google Scholar 

Slater EC (1973) The mechanism of action of the respiratory inhibitor, antimycin  Biochim Biophys Acta 301:129–154. https://doi.org/10.1016/0304-4173(73)90002-5

Article  CAS  PubMed  Google Scholar 

Kenwood BM, Weaver JL, Bajwa A, Poon IK, Byrne FL, Murrow BA, Calderone JA, Huang L, Divakaruni AS, Tomsig JL, Okabe K, Lo RH, Cameron Coleman G, Columbus L, Yan Z, Saucerman JJ, Smith JS, Holmes JW, Lynch KR, Ravichandran KS, Uchiyama S, Santos WL, Rogers GW, Okusa MD, Bayliss DA, Hoehn KL (2014) Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane. Mol Metabolism 3:114–123. https://doi.org/10.1016/j.molmet.2013.11.005

Article  CAS  Google Scholar 

Schmidt M, Dringen R (2009) Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes. Front Neuroenergetics 1:1–10. https://doi.org/10.3389/neuro.14.001.2009

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ghergurovich JM, García-Cañaveras JC, Wang J, Schmidt E, Zhang Z, TeSlaa T, Patel H, Chen L, Britt EC, Piqueras-Nebot M, Gomez-Cabrera MC, Lahoz A, Fan J, Beier UH, Kim H, Rabinowitz JD (2020) A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol 16:731–739. https://doi.org/10.1038/s41589-020-0533-x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dehn DL, Siegel D, Swann E, Moody CJ, Ross D (2003) Biochemical, cytotoxic, and genotoxic effects of ES936, a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1, in cellular systems. Mol Pharmacol 64:714–720. https://doi.org/10.1124/mol.64.3.714

Article  CAS  PubMed  Google Scholar 

Timson DJ (2017) Dicoumarol: a drug which hits at least two very different targets in vitamin K metabolism. Curr Drug Targets 18:500–510. https://doi.org/10.2174/1389450116666150722141906

Article  CAS  PubMed  Google Scholar 

Baxter PS, Hardingham GE (2016) Adaptive regulation of the brain’s antioxidant defences by neurons and astrocytes. Free Radic Biol Med 100:147–152. https://doi.org/10.1016/j.freeradbiomed.2016.06.027

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu CS, Chang CK, Lin LS, Jou SB, KUo DH, Liao SS, Cheng JT (1998) Modification of superoxide dismutase (SOD) mRNA and activity by a transient hypoxic stress in cultured glial cells. Neurosci Lett 251:145–148. https://doi.org/10.1016/S0304-3940(98)00506-0

Article  Google Scholar 

Dringen R, Hamprecht B (1997) Involvement of glutathione peroxidase and catalase in the disposal of exogenous hydrogen peroxide by cultured astroglial cells. Brain Res 759:67–75. https://doi.org/10.1016/s0006-8993(97)00233-3

Article  CAS  PubMed  Google Scholar 

留言 (0)

沒有登入
gif