World Health Organization (WHO). Global health sector strategy on sexually transmitted infections 2016–2021: toward ending STIs. Geneva: WHO; 2016. Available from: https://www.who.int/publications/i/item/WHO-RHR-16.09

Unemo M, et al. Gonorrhoea. Nat Rev Dis Prim. 2019;5:79.

Article  PubMed  Google Scholar 

Unemo M, Shafer WM. Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future. Clin Microbiol Rev. 2014;27:587–613.

Article  PubMed  PubMed Central  Google Scholar 

WHO Guidelines for the Treatment of Neisseria gonorrhoeae. 2016; https://apps.who.int/iris/bitstream/handle/10665/246114/9789241549691-eng.pdf

Unemo M, et al. WHO global antimicrobial resistance surveillance for Neisseria gonorrhoeae 2017–18: a retrospective observational study. Lancet Microbe. 2021;2:e627–36.

Article  PubMed  Google Scholar 

Eyre DW, et al. Gonorrhoea treatment failure caused by a Neisseria gonorrhoeae strain with combined ceftriaxone and high-level azithromycin resistance, England, February 2018. Eur Surveill. 2018;23:1800323.

Article  Google Scholar 

Food and Drug Administration HHS. Determination that ALBAMYCIN (novobiocin sodium) capsule, 250 milligrams, was withdrawn from sale for reasons of safety or effectiveness. Federal Register. 2011:76;3143–4

Maxwell A. The interaction between coumarin drugs and DNA gyrase. Mol Microbiol. 1993;9:681–6.

Article  PubMed  Google Scholar 

Duffin PM, Steven Seifert H. DNA uptake sequence-mediated enhancement of transformation in Neisseria gonorrhoeae is strain dependent. J Bacteriol. 2010;192:4436–44.

Article  PubMed  PubMed Central  Google Scholar 

Miyazaki K. Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques. 2015;58:86–8.

Article  PubMed  Google Scholar 

Kino Y, et al. Counterselection employing mutated pheS for markerless genetic deletion in Bacteroides species. Anaerobe. 2016;42:81–8.

Article  PubMed  Google Scholar 

Gao G, et al. Highly effective markerless genetic manipulation of Streptococcus suis using a mutated PheS-based counterselectable marker. Front Microbiol. 2022;13:947821.

Article  PubMed  PubMed Central  Google Scholar 

McNutt AT, et al. GNINA 1.0: molecular docking with deep learning. J Cheminform. 2021;13:43.

Article  PubMed  PubMed Central  Google Scholar 

Trott O, Olson AJ. Autodock vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2010;31:455–61.

Article  PubMed  PubMed Central  Google Scholar 

Koes DR, Baumgartner MP, Camacho CJ. Lessons learned in empirical scoring with smina from the csar 2011 benchmarking exercise. J Chem Inform Model. 2013;53:1893–904.

Article  Google Scholar 

Gross CH, et al. Active-site residues of Escherichia coli DNA gyrase required in coupling ATP hydrolysis to DNA supercoiling and amino acid substitutions leading to novobiocin resistance. Antimicrob Agents Chemother. 2003;47:1037–46.

Article  PubMed  PubMed Central  Google Scholar 

Fujimoto-Nakamura M, Ito H, Oyamada H, Nishino T, Yamagishi J. Accumulation of mutations in both gyrB and parE genes is associated with high-level resistance to novobiocin in Staphylococcus aureus. Antimicrob Agents Chemother. 2005;49:3810–5.

Article  PubMed  PubMed Central  Google Scholar 

Chopra S, et al. Evaluation of gyrase B as a drug target in Mycobacterium tuberculosis. J Antimicrob Chemother. 2012;67:415–21.

Article  PubMed  Google Scholar 

Chitsaz M, Booth L, Blyth MT, O’Mara ML, Brown MH. Multidrug resistance in Neisseria gonorrhoeae: identification of functionally important residues in the MtrD Efflux Protein. mBio. 2019;10:e02277–19.

Article  PubMed  PubMed Central  Google Scholar 

Bellon S, et al. Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase. Antimicrob Agents Chemother. 2004;48:1856–64.

Article  PubMed  PubMed Central  Google Scholar 

Charifson PS, et al. Novel dual-targeting benzimidazole urea inhibitors of DNA gyrase and topoisomerase IV possessing potent antibacterial activity: intelligent design and evolution through the judicious use of structure-guided design and structure-activity relationships. J Med Chem. 2008;51:5243–63.

Article  PubMed  Google Scholar