Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes

Roca-Cusachs, P., Conte, V. & Trepat, X. Quantifying forces in cell biology. Nat. Cell Biol. 19, 742–751 (2017).

Article  CAS  PubMed  Google Scholar 

Abella, M., Andruck, L., Malengo, G. & Skruzny, M. Actin-generated force applied during endocytosis measured by Sla2-based FRET tension sensors. Dev. Cell 56, 2419–2426.e4 (2021).

Article  CAS  PubMed  Google Scholar 

Déjardin, T. et al. Nesprins are mechanotransducers that discriminate epithelial–mesenchymal transition programs. J. Cell Biol. 219, e201908036 (2020).

Article  PubMed  PubMed Central  Google Scholar 

Li, W. et al. A membrane-bound biosensor visualizes shear stress-induced inhomogeneous alteration of cell membrane tension. iScience 7, 180–190 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Páez-Pérez, M., López-Duarte, I., Vyšniauskas, A., Brooks, N. J. & Kuimova, M. K. Imaging non-classical mechanical responses of lipid membranes using molecular rotors. Chem. Sci. 12, 2604–2613 (2021).

Article  Google Scholar 

Assies, L. et al. Flipper probes for the community. Chim. Int. J. Chem. 75, 1004–1011 (2021).

Article  CAS  Google Scholar 

Soleimanpour, S. et al. Headgroup engineering in mechanosensitive membrane probes. Chem. Commun. 52, 14450–14453 (2016).

Article  CAS  Google Scholar 

Colom, A. et al. A fluorescent membrane tension probe. Nat. Chem. 10, 1118–1125 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mercier, V. et al. Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Nat. Cell Biol. 22, 947–959 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Roffay, C. et al. Passive coupling of membrane tension and cell volume during active response of cells to osmosis. Proc. Natl Acad. Sci. USA 118, e2103228118 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ni, Q. et al. Cytoskeletal activation of NHE1 regulates cell volume and DNA methylation. Preprint at bioRxiv https://doi.org/10.1101/2023.08.31.555808 (2023).

Michels, L. et al. Complete microviscosity maps of living plant cells and tissues with a toolbox of targeting mechanoprobes. Proc. Natl Acad. Sci. USA 117, 18110–18118 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Coomer, C. A. et al. Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion. PLoS Pathog. 16, e1008359 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jiménez-Rojo, N. et al. Conserved functions of ether lipids and sphingolipids in the early secretory pathway. Curr. Biol. 30, 3775–3787.e7 (2020).

Article  PubMed  Google Scholar 

Mylvaganam, S. et al. The spectrin cytoskeleton integrates endothelial mechanoresponses. Nat. Cell Biol. 24, 1226–1238 (2022).

Article  CAS  PubMed  Google Scholar 

Lachuer, H., Le, L., Lévêque-Fort, S., Goud, B. & Schauer, K. Spatial organization of lysosomal exocytosis relies on membrane tension gradients. Proc. Natl Acad. Sci. 120, e2207425120 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lachowski, D. et al. Substrate stiffness-driven membrane tension modulates vesicular trafficking via caveolin-1. ACS Nano 16, 4322–4337 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Riggi, M. et al. Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2. Nat. Cell Biol. 20, 1043–1051 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Riggi, M., Kusmider, B. & Loewith, R. The flipside of the TOR coin—TORC2 and plasma membrane homeostasis at a glance. J. Cell Sci. 133, jcs242040 (2020).

Article  CAS  PubMed  Google Scholar 

Wang, S. et al. Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice. Nat. Commun. 11, 2303 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nava, M. M. et al. Heterochromatin-driven nuclear softening protects the genome against mechanical stress-induced damage. Cell 181, 800–817.e22 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Schneider, A. F. L., Kithil, M., Cardoso, M. C., Lehmann, M. & Hackenberger, C. P. R. Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives. Nat. Chem. 13, 530–539 (2021).

Article  CAS  PubMed  Google Scholar 

Hetmanski, J. H. R. et al. Membrane tension orchestrates rear retraction in matrix-directed cell migration. Dev. Cell 51, 460–475.e10 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yavitt, F. M. et al. In situ modulation of intestinal organoid epithelial curvature through photoinduced viscoelasticity directs crypt morphogenesis. Sci. Adv. 9, eadd5668 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Goujon, A. et al. Mechanosensitive fluorescent probes to image membrane tension in mitochondria, endoplasmic reticulum, and lysosomes. J. Am. Chem. Soc. 141, 3380–3384 (2019).

Article  CAS  PubMed  Google Scholar 

Dal Molin, M. et al. Fluorescent flippers for mechanosensitive membrane probes. J. Am. Chem. Soc. 137, 568–571 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

García-Sáez, A. J., Chiantia, S. & Schwille, P. Effect of line tension on the lateral organization of lipid membranes. J. Biol. Chem. 282, 33537–33544 (2007).

Article  PubMed  Google Scholar 

Hamada, T., Kishimoto, Y., Nagasaki, T. & Takagi, M. Lateral phase separation in tense membranes. Soft Matter 7, 9061–9068 (2011).

Article  CAS  Google Scholar 

Shimokawa, N. & Hamada, T. Physical concept to explain the regulation of lipid membrane phase separation under isothermal conditions. Life 13, 1105 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

García-Calvo, J. et al. HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells. Chem. Sci. 13, 2086–2093 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Licari, G., Strakova, K., Matile, S. & Tajkhorshid, E. Twisting and tilting of a mechanosensitive molecular probe detects order in membranes. Chem. Sci. 11, 5637–5649 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Harris, F. M., Best, K. B. & Bell, J. D. Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order. Biochim. Biophys. Acta 1565, 123–128 (2002).

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