Arcilla MS, van Hattem JM, Haverkate MR, Bootsma MC, van Genderen PJ, Goorhuis A, Grobusch MP, Lashof AMO, Molhoek N, Schultsz C, Stobberingh EE, Verbrugh HA, de Jong MD, Melles DC, Penders J. Import and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae by international travellers (COMBAT study): a prospective, multicentre cohort study. Lancet Infect Dis. 2017;17:78–85.

Article  PubMed  Google Scholar 

Ahmed W, Smith WJM, Metcalfe S, Jackson G, Choi PM, Morrison M, Field D, Gyawali P, Bivins A, Bibby K, Simpson SL. Comparison of RT-qPCR and RT-dPCR platforms for the trace detection of SARS-CoV-2 RNA in wastewater. ACS ES&T Water. 2022;11:1871–80.

Article  Google Scholar 

Akter J, Smith WJ, Liu Y, Kim I, Simpson S, Thai P, Korajkic A, Ahmed W. Comparison of adsorption-extraction workflows for improved measurements of viruses and bacteria in untreated wastewater. Sci Total Environ. 2023;908: 167966.

Article  PubMed  Google Scholar 

Barnes MA, Turner CR, Jerde CL, Renshaw MA, Chadderton WL, Lodge DM. Environmental conditions influence eDNA persistence in aquatic systems. Environ Sci Technol. 2014;48:1819–27.

Article  CAS  PubMed  Google Scholar 

Berendonk TU, Manaia CM, Merlin C, Fatta-Kassinos D, Cytryn E, Walsh F, Bürgmann H, Sørum H, Norström M, Pons MN, Kreuzinger N, Huovinen P, Stefani S, Schwartz T, Kisand V, Baquero F, Martinez JL. Tackling antibiotic resistance: the environmental framework. Nat Rev Microbiol. 2015;13:310–7.

Article  CAS  PubMed  Google Scholar 

Bivins A, Morfino R, Franklin A, Simspson S, Ahmed W. The lavator lens: tracking the global movement of pathogens via aircraft wastewater. Crit. Rev. Environ. Sci. Technol. 2023;54:321–41.

Article  Google Scholar 

Bramucci AR, Focardi A, Rinke C, Hugenholtz P, Tyson GW, Seymour JR, Raina JB. Microvolume DNA extraction methods for microscale amplicon and metagenomic studies. ISME Commun. 2021;17:79.

Article  Google Scholar 

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55:611–22.

Article  CAS  PubMed  Google Scholar 

Centers for Disease Control and Prevention (CDC). Antibiotic resistance threats in the United States, 2019. U.S. Department of Health and Human Services, CDC, Atlanta, GA.

D’Costa VM, King CE, Kalan L, Morar M, Sung WW, Schwarz C, Froese D, Zazula G, Calmels F, Debruyne R, Golding GB, Poinar HN, Wright GD. Antibiotic resistance is ancient. Nature. 2011;477:457–61.

Article  CAS  PubMed  Google Scholar 

Demeke T, Jenkins GR. Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal Bioanal Chem. 2010;396:1977–90.

Article  CAS  PubMed  Google Scholar 

Elleuch A, Fakhfakh H, Jendoubi L, Bessaies N, Marrakchi M. Comparative analysis of techniques for detection of grapevine and citrus viroids in Tunisia. EPPO Bull. 2003;33:369–74.

Article  Google Scholar 

Frost I, Van Boeckel TP, Pires J, Craig J, Laxminarayan R. Global geographic trends in antimicrobial resistance: The role of international travel. J Trav Med. 2019;26:taz036. 

Article  Google Scholar 

Haugland RA, Siefring SC, Wymer LJ, Brenner KP, Dufour AP. Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis. Water Res. 2005;39:559–68.

Article  CAS  PubMed  Google Scholar 

Heß S, Kneis D, Österlund T, Li B, Kristiansson E, Berendonk TU. Sewage from airplanes exhibits high abundance and diversity of antibiotic resistance genes. Environ Sci Technol. 2019;53:13898–905.

Article  PubMed  Google Scholar 

Hendriksen RS, Munk P, Njage P, van Bunnik B, McNally L, Lukjancenko O, Röder T, Nieuwenhuijse D, Pedersen SK, Kjeldgaard J, Kaas RS, Clausen PTLC, Vogt JK, Leekitcharoenphon P, van de Schans MGM, Zuidema T, de Roda Husman AM, Rasmussen S, Petersen B; Global Sewage Surveillance project consortium; Amid C, Cochrane G, Sicheritz-Ponten T, Schmitt H, Alvarez JRM, Aidara-Kane A, Pamp SJ, Lund O, Hald T, Woolhouse M, Koopmans MP, Vigre H, Petersen TN, Aarestrup FM. Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage. Nat Commun. 2019;10:1124

IATA. Air Passenger Monthly Analysis – August 2023. IATA.org. Retrieved from https://www.iata.org/en/iata-repository/publications/economic-reports/air-passenger-market-analysis---august-2023/

Keenum I, Liguori K, Calarco J, Davis BC, Milligan E, Harwood VJ, Pruden A. A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater. Crit Rev Environ Sci Technol. 2022;52:4395–419.

Article  CAS  Google Scholar 

Li AD, Metch JW, Wang Y, Garner E, Zhang AN, Riquelme MV, Vikesland PJ, Pruden A, Zhang T. Effects of sample preservation and DNA extraction on enumeration of antibiotic resistance genes in wastewater. FEMS Microbiol Ecol. 2018;94.

Li J, Hosegood I, Powell D, Tscharke B, Lawler J, Thomas KV, Mueller JF. A global aircraft-based wastewater genomic surveillance network for early warning of future pandemics. Lancet Glob Health. 2023;11:791–5.

Article  Google Scholar 

Liguori K, Keenum I, Davis BC, Calarco J, Milligan E, Harwood VJ, Pruden A. Antimicrobial resistance monitoring of water environments: a framework for standardized methods and quality control. Environ Sci Technol. 2022;56:9149–60.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu Y, Smith W, Gebrewold M, Wang X, Simpson SL, Bivins A, Ahmed W. Comparison of concentration and extraction workflows for qPCR quantification of intI1 and vanA in untreated wastewater. Sci Total Environ. 2023;903: 166442.

Article  CAS  PubMed  Google Scholar 

Makowska N, Philips A, Dabert M, Nowis K, Trzebny A, Koczura R, Mokracka J. Metagenomic analysis of β-lactamase and carbapenemase genes in the wastewater resistome. Water Res. 2020;170: 115277.

Article  CAS  PubMed  Google Scholar 

Nordahl Petersen T, Rasmussen S, Hasman H, Carøe C, Bælum J, Schultz AC, Bergmark L, Svendsen CA, Lund O, Sicheritz-Pontén T, Aarestrup FM. Meta-genomic analysis of toilet waste from long distance flights; a step towards global surveillance of infectious diseases and antimicrobial resistance. Sci Rep. 2015;5:11444.

Article  PubMed  PubMed Central  Google Scholar 

Ojer-Usoz E, González D, García-Jalón I, Vitas AI. High dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae in effluents from wastewater treatment plants. Water Res. 2014;56:37–47.

Article  CAS  PubMed  Google Scholar 

Pereira DG, Afonso A, Medeiros FM. Overview of Friedman’s test and post-hoc analysis. Commun Stat Simul. 2015;44(10):2636–53.

Article  Google Scholar 

Pruden A, Arabi M, Storteboom HN. Correlation between upstream human activities and riverine antibiotic resistance genes. Environ Sci Technol. 2012;46:11541–9.

Article  CAS  PubMed  Google Scholar 

Stocker R. Marine microbes see a sea of gradients. Science. 2012;338:628–33.

Article  CAS  PubMed  Google Scholar 

Staley C, Gordon KV, Schoen ME, Harwood VJ. Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters. Appl Environ Microbiol. 2012;78:7317–26.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Toh SM, Xiong L, Arias CA, Villegas MV, Lolans K, Quinn J, Mankin AS. Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. Mol Microbiol. 2007;64:1506–14.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Triant DA, Whitehead A. Simultaneous extraction of high-quality RNA and DNA from small tissue samples. J Hered. 2009;100(2):246–50.

Article  CAS  PubMed  Google Scholar 

von Wintersdorff CJ, Penders J, Stobberingh EE, Oude Lashof AM, Hoebe CJ, Savelkoul PH, Wolffs PF. High rates of antimicrobial drug resistance gene acquisition after international travel. The Netherlands Emerg Infect Dis. 2014;20:649–57.

Article  Google Scholar 

World Health Organization. Global action plan on antimicrobial resistance. Geneva: World Health Organization; 2015.

Google Scholar 

Wu JJ, Ko WC, Tsai SH, Yan JJ. Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital. Antimicrob Agents Chemother. 2007;51:1223–7.

Article  CAS  PubMed  PubMed Central  Google Scholar