Swayne, D., Suarez, D. & Sims, L. Influenza in Diseases of Poultry (ed. Swayne, D. E.) 210-256 (John Wiley & Sons, Inc., 2020).

Pantin-Jackwood, M. J. & Swayne, D. E. Pathogenesis and pathobiology of avian influenza virus infection in birds. Rev. Sci. Tech. 28, 113–136 (2009).

Article  CAS  PubMed  Google Scholar 

Swayne, D. E. & Kapczynski, D. R. Vaccines And Vaccination For Avian Influenza In Poultry in Animal Influenza (ed Swayne, D. E.) 378-434 (John Wiley & Sons, Inc., 2017).

Suarez, D. L. & Pantin-Jackwood, M. J. Recombinant viral-vectored vaccines for the control of avian influenza in poultry. Vet. Microbiol. 206, 144–151 (2017).

Article  CAS  PubMed  Google Scholar 

Tian, G. et al. Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics. Virology 341, 153–162 (2005).

Article  CAS  PubMed  Google Scholar 

van der Goot, J. A., Koch, G., de Jong, M. C. & van Boven, M. Quantification of the effect of vaccination on transmission of avian influenza (H7N7) in chickens. Proc. Natl. Acad. Sci. USA. 102, 18141–18146 (2005).

Article  PubMed  PubMed Central  Google Scholar 

Ge, J. et al. Newcastle disease virus-based live attenuated vaccine completely protects chickens and mice from lethal challenge of homologous and heterologous H5N1 avian influenza viruses. J. Virol. 81, 150–158 (2007).

Article  CAS  PubMed  Google Scholar 

Bublot, M. et al. Efficacy of a fowlpox-vectored avian influenza H5 vaccine against Asian H5N1 highly pathogenic avian influenza virus challenge. Avian Dis 51, 498–500 (2007).

Article  PubMed  Google Scholar 

Gardin, Y. et al. Experimental and field results regarding immunity induced by a recombinant turkey herpesvirus H5 vector vaccine against H5N1 and other H5 highly pathogenic avian influenza virus challenges. Avian Dis 60, 232–237 (2016).

Article  PubMed  Google Scholar 

Kolpe, A., Schepens, B., Fiers, W. & Saelens, X. M2-based influenza vaccines: recent advances and clinical potential. Expert Rev. Vaccines 16, 123–136 (2017).

Article  CAS  PubMed  Google Scholar 

Opriessnig, T. et al. An experimental universal swine influenza A virus (IAV) vaccine candidate based on the M2 ectodomain (M2e) peptide does not provide protection against H1N1 IAV challenge in pigs. Vaccine 42, 220–228 (2024).

Article  PubMed  Google Scholar 

Heinen, P. P., Rijsewijk, F. A., de Boer-Luijtze, E. A. & Bianchi, A. T. J. Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus. J. Gen. Virol. 83, 1851–1859 (2002).

Article  CAS  PubMed  Google Scholar 

Bernelin-Cottet, C. et al. A universal influenza vaccine can lead to disease exacerbation or viral control depending on delivery strategies. Front. Immunol. 7, 641 (2016).

Article  PubMed  PubMed Central  Google Scholar 

Zhang, Z. et al. Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus. Virol. J. 7, 89 (2010).

Article  PubMed  PubMed Central  Google Scholar 

Hajam, I. A., Kim, J. & Lee, J. H. Intranasally administered polyethylenimine adjuvanted influenza M2 ectodomain induces partial protection against H9N2 influenza A virus infection in chickens. Vet. Immunol. Immunopathol. 209, 78–83 (2019).

Article  CAS  PubMed  Google Scholar 

Babapoor, S. et al. A novel vaccine using nanoparticle platform to present immunogenic M2e against avian influenza infection. Influenza Res. Treat. 2011, 126794 (2011).

PubMed  Google Scholar 

Dabaghian, M. et al. Vaccination with recombinant 4 x M2e.HSP70c fusion protein as a universal vaccine candidate enhances both humoral and cell-mediated immune responses and decreases viral shedding against experimental challenge of H9N2 influenza in chickens. Vet. Microbiol. 174, 116–126 (2014).

Article  CAS  PubMed  Google Scholar 

Elaish, M. et al. Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens. Vaccine 33, 4901–4909 (2015).

Article  CAS  PubMed  Google Scholar 

Elaish, M. et al. Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens. PLoS ONE 12, e0171174 (2017).

Article  PubMed  PubMed Central  Google Scholar 

Elaish, M. et al. Protective immunity against influenza virus challenge by norovirus P particle-M2e and HA2-AtCYN vaccines in chickens. Vaccine 37, 6454–6462 (2019).

Article  CAS  PubMed  Google Scholar 

Hajam, I. A., Senevirathne, A., Hewawaduge, C., Kim, J. & Lee, J. H. Intranasally administered protein coated chitosan nanoparticles encapsulating influenza H9N2 HA2 and M2e mRNA molecules elicit protective immunity against avian influenza viruses in chickens. Vet. Res. 51, 37 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tang, Y. et al. Chimaeric VP2 proteins from infectious bursal disease virus containing the N-terminal M2e of H9 subtype avian influenza virus induce neutralizing antibody responses to both viruses. Avian Pathol. 42, 260–267 (2013).

Article  CAS  PubMed  Google Scholar 

Hajam, I. A., Kim, J. & Lee, J. H. Salmonella Gallinarum delivering M2eCD40L in protein and DNA formats acts as a bivalent vaccine against fowl typhoid and H9N2 infection in chickens. Vet. Res. 49, 99 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Kim, J. H., Hajam, I. A. & Lee, J. H. Oral immunization with a novel attenuated Salmonella Typhimurium encoding influenza HA, M2e and NA antigens protects chickens against H7N9 infection. Vet. Res. 49, 12 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Layton, S. L. et al. Vaccination of chickens with recombinant Salmonella expressing M2e and CD154 epitopes increases protection and decreases viral shedding after low pathogenic avian influenza challenge. Poult. Sci. 88, 2244–2252 (2009).

Article  CAS  PubMed  Google Scholar 

Yang, W. T. et al. Lactobacillus plantarum displaying conserved M2e and HA2 fusion antigens induces protection against influenza virus challenge. Appl. Microbiol. Biotechnol. 102, 5077–5088 (2018).

Article  CAS  PubMed  Google Scholar 

Hajam, I. A. et al. Oral immunization with an attenuated Salmonella Gallinarum encoding the H9N2 haemagglutinin and M2 ectodomain induces protective immune responses against H9N2 infection in chickens. Avian Pathol. 49, 486–495 (2020).

Article  CAS  PubMed  Google Scholar 

Kalaiyarasu, S. et al. Elicitation of highly pathogenic avian influenza H5N1 M2e and HA2-specific humoral and cell-mediated immune response in chicken following immunization with recombinant M2e-HA2 fusion orotein. Front. Vet. Sci. 7, 571999 (2020).

Article  PubMed  Google Scholar 

Li, J. et al. Nanoparticle vaccine for avian influenza virus: a challenge study against highly pathogenic H5N2 subtype. J. Virol. Antivir. Res. 7, 1 (2018).

Article  Google Scholar 

Reese, K. A. et al. A novel lactococcal vaccine expressing a peptide from the M2 antigen of H5N2 highly pathogenic avian influenza A virus prolongs survival of vaccinated chickens. Vet. Med. Int. 2013, 316926 (2013).

Article  PubMed  PubMed Central  Google Scholar 

Song, B. M. et al. Supplemented vaccination with tandem repeat M2e virus-like particles enhances protection against homologous and heterologous HPAI H5 viruses in chickens. Vaccine 34, 678–686 (2016).

Article  CAS  PubMed  Google Scholar 

Zhang, X. et al. Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology 54, 290–299 (2011).

Article  CAS  PubMed  Google Scholar 

Zhang, Z. et al. Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus. Vet. Microbiol. 203, 143–148 (2017).

Article