Lee, R. C., Feinbaum, R. L. & Ambros, V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75, 843–854 (1993).

Article  CAS  PubMed  Google Scholar 

Wightman, B., Ha, I. & Ruvkun, G. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75, 855–862 (1993).

Article  CAS  PubMed  Google Scholar 

Bartel, D. P. Metazoan microRNAs. Cell 173, 20–51 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gebert, L. F. R. & MacRae, I. J. Regulation of microRNA function in animals. Nat. Rev. Mol. Cell Biol. 20, 21–37 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Iwakawa, H.-O. & Tomari, Y. Life of RISC: formation, action, and degradation of RNA-induced silencing complex. Mol. Cell 82, 30–43 (2022).

Article  CAS  PubMed  Google Scholar 

Friedman, R. C., Farh, K. K.-H., Burge, C. B. & Bartel, D. P. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 19, 92–105 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee, Y. et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 23, 4051–4060 (2004).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cai, X., Hagedorn, C. H. & Cullen, B. R. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10, 1957–1966 (2004).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hutvágner, G. et al. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293, 834–838 (2001).

Article  PubMed  Google Scholar 

Ketting, R. F. et al. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 15, 2654–2659 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bernstein, E., Caudy, A. A., Hammond, S. M. & Hannon, G. J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363–366 (2001).

Article  CAS  PubMed  Google Scholar 

Grishok, A. et al. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106, 23–34 (2001).

Article  CAS  PubMed  Google Scholar 

Knight, S. W. & Bass, B. L. A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 293, 2269–2271 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee, Y., Jeon, K., Lee, J.-T., Kim, S. & Kim, V. N. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 21, 4663–4670 (2002).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee, Y. et al. The nuclear RNase III Drosha initiates microRNA processing. Nature 425, 415–419 (2003).

Article  CAS  PubMed  Google Scholar 

Han, J. et al. The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 18, 3016–3027 (2004).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gregory, R. I. et al. The Microprocessor complex mediates the genesis of microRNAs. Nature 432, 235–240 (2004).

Article  CAS  PubMed  Google Scholar 

Denli, A. M., Tops, B. B. J., Plasterk, R. H. A., Ketting, R. F. & Hannon, G. J. Processing of primary microRNAs by the Microprocessor complex. Nature 432, 231–235 (2004).

Article  CAS  PubMed  Google Scholar 

Landthaler, M., Yalcin, A. & Tuschl, T. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis. Curr. Biol. 14, 2162–2167 (2004).

Article  CAS  PubMed  Google Scholar 

Tabara, H. et al. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123–132 (1999).

Article  CAS  PubMed  Google Scholar 

Hammond, S. M., Boettcher, S., Caudy, A. A., Kobayashi, R. & Hannon, G. J. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 293, 1146–1150 (2001).

Article  CAS  PubMed  Google Scholar 

Mourelatos, Z. et al. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev. 16, 720–728 (2002).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shang, R., Lee, S., Senavirathne, G. & Lai, E. C. microRNAs in action: biogenesis, function and regulation. Nat. Rev. Genet. 24, 816–833 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ha, M. & Kim, V. N. Regulation of microRNA biogenesis. Nat. Rev. Mol. Cell Biol. 15, 509–524 (2014).

Article  CAS  PubMed  Google Scholar 

Ruby, J. G., Jan, C. H. & Bartel, D. P. Intronic microRNA precursors that bypass Drosha processing. Nature 448, 83–86 (2007).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Okamura, K., Hagen, J. W., Duan, H., Tyler, D. M. & Lai, E. C. The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. Cell 130, 89–100 (2007).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cheloufi, S., Dos Santos, C. O., Chong, M. M. W. & Hannon, G. J. A Dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature 465, 584–589 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cifuentes, D. et al. A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science 328, 1694–1698 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xie, M. et al. Mammalian 5′-capped microRNA precursors that generate a single microRNA. Cell 155, 1568–1580 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ender, C. et al. A human snoRNA with microRNA-like functions. Mol. Cell 32, 519–528 (2008).

Article  CAS  PubMed