Azouzi C, Jaafar M, Dez C, Abou Merhi R, Lesne A, Henras AK, Gadal O (2021) Coupling between production of ribosomal RNA and maturation: just at the beginning. Front Mol Biosci 8:778778. https://doi.org/10.3389/fmolb.2021.778778

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ban N, Nissen P, Hansen J, Moore PB, Steitz TA (2000) The complete atomic structure of the large ribosomal subunit at 2.4 a resolution. Science. 289(5481):905–20. https://doi.org/10.1126/science.289.5481.905

Article  CAS  PubMed  Google Scholar 

Bizhanova A, Kaufman PD (2021) Close to the edge: Heterochromatin at the nucleolar and nuclear peripheries. Biochim Biophys Acta Gene Regul Mech. 1864(1):194666. https://doi.org/10.1016/j.bbagrm.2020.194666

Article  CAS  PubMed  Google Scholar 

Boulon S, Westman BJ, Hutten S, Boisvert FM, Lamond AI (2010) The nucleolus under stress. Mol Cell 40(2):216–227. https://doi.org/10.1016/j.molcel.2010.09.024

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bruno PM, Liu Y, Park GY, Murai J, Koch CE, Eisen TJ, Pritchard JR, Pommier Y, Lippard SJ, Hemann MT (2017) A subset of platinum-containing chemotherapeutic agents kills cells by inducing ribosome biogenesis stress. Nat Med. 23(4):461–471. https://doi.org/10.1038/nm.4291

Article  CAS  PubMed  PubMed Central  Google Scholar 

Burger K, Mühl B, Rohrmoser M, Coordes B, Heidemann M, Kellner M, Gruber-Eber A, Heissmeyer V, Strässer K, Eick D (2013) Cyclin-dependent kinase 9 links RNA polymerase II transcription to processing of ribosomal RNA. J Biol Chem. 288(29):21173–21183. https://doi.org/10.1074/jbc.M113.483719

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bursać S, Prodan Y, Pullen N, Bartek J, Volarević S (2021) Dysregulated ribosome biogenesis reveals therapeutic liabilities in cancer. Trends Cancer 7(1):57–76. https://doi.org/10.1016/j.trecan.2020.08.003

Article  CAS  PubMed  Google Scholar 

Chen HK, Pai CY, Huang JY, Yeh NH (1999) Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization. Mol Cell Biol 19(12):8536–8546. https://doi.org/10.1128/MCB.19.12.8536

Article  CAS  PubMed  PubMed Central  Google Scholar 

Correll CC, Bartek J, Dundr M (2019) The nucleolus: a multiphase condensate balancing ribosome synthesis and translational capacity in health aging and ribosomopathies. Cells 8(8):869. https://doi.org/10.3390/cells8080869

Article  CAS  PubMed  PubMed Central  Google Scholar 

Daiß JL, Pilsl M, Straub K, Bleckmann A, Höcherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tlučková K, Mars JC, Fürtges T, Bruckmann A, Rudack T, Bernecky C, Lamour V, Panov K, Vannini A, Moss T, Engel C (2022) The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans. Life Sci Alliance 5(11):e202201568. https://doi.org/10.26508/lsa.202201568

Article  CAS  PubMed  PubMed Central  Google Scholar 

Denissov S, Lessard F, Mayer C, Stefanovsky V, van Driel M, Grummt I, Moss T, Stunnenberg HG (2011) A model for the topology of active ribosomal RNA genes. EMBO Rep 12(3):231–237. https://doi.org/10.1038/embor.2011.8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Derenzini M, Trerè D, Pession A, Montanaro L, Sirri V, Ochs RL (1998) Nucleolar function and size in cancer cells. Am J Pathol. 152(5):1291–7

CAS  PubMed  PubMed Central  Google Scholar 

Dorado TE, de León P, Begum A, Liu H, Chen D, Rajeshkumar NV, Rey-Rodriguez R, Hoareau-Aveilla C, Alcouffe C, Laiho M, Barrow JC (2022) Discovery and evaluation of novel angular fused pyridoquinazolinonecarboxamides as RNA polymerase I inhibitors. ACS Med Chem Lett 13(4):608–614. https://doi.org/10.1021/acsmedchemlett.1c00660

Article  CAS  PubMed  PubMed Central  Google Scholar 

Drygin D, Siddiqui-Jain A, O’Brien S, Schwaebe M, Lin A, Bliesath J, Ho CB, Proffitt C, Trent K, Whitten JP, Lim JK, Von Hoff D, Anderes K, Rice WG (2009) Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis. Cancer Res 69(19):7653–7661. https://doi.org/10.1158/0008-5472.CAN-09-1304

Article  CAS  PubMed  Google Scholar 

Dundr M, Olson MO (1998) Partially processed pre-rRNA is preserved in association with processing components in nucleolus-derived foci during mitosis. Mol Biol Cell 9(9):2407–2422. https://doi.org/10.1091/mbc.9.9.2407

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dundr M, Hoffmann-Rohrer U, Hu Q, Grummt I, Rothblum LI, Phair RD, Misteli T (2002) A kinetic framework for a mammalian RNA polymerase in vivo. Science 298(5598):1623–1626. https://doi.org/10.1126/science.1076164

Article  CAS  PubMed  Google Scholar 

Elhamamsy AR, Metge BJ, Alsheikh HA, Shevde LA, Samant RS (2022) Ribosome biogenesis: a central player in cancer metastasis and therapeutic resistance. Cancer Res 82(13):2344–2353. https://doi.org/10.1158/0008-5472.CAN-21-4087

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ferreira R, Schneekloth JS Jr, Panov KI, Hannan KM, Hannan RD (2020) Targeting the RNA polymerase I transcription for cancer therapy comes of age. Cells 9(2):266. https://doi.org/10.3390/cells9020266

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ferrolino MC, Mitrea DM, Michael JR, Kriwacki RW (2018) Compositional adaptability in NPM1-SURF6 scaffolding networks enabled by dynamic switching of phase separation mechanisms. Nat Commun 9(1):5064. https://doi.org/10.1038/s41467-018-07530-1

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ford BL, Wei T, Liu H, Scull CE, Najmi SM, Pitts S, Fan W, Schneider DA, Laiho M (2023) Expression of RNA polymerase I catalytic core is influenced by RPA12. PLoS ONE 18(5):e0285660. https://doi.org/10.1371/journal.pone.0285660

Article  CAS  PubMed  PubMed Central  Google Scholar 

Frankowski KJ, Wang C, Patnaik S, Schoenen FJ, Southall N, Li D, Teper Y, Sun W, Kandela I, Hu D, Dextras C, Knotts Z, Bian Y, Norton J, Titus S, Lewandowska MA, Wen Y, Farley KI, Griner LM, Sultan J, Meng Z, Zhou M, Vilimas T, Powers AS, Kozlov S, Nagashima K, Quadri HS, Fang M, Long C, Khanolkar O, Chen W, Kang J, Huang H, Chow E, Goldberg E, Feldman C, Xi R, Kim HR, Sahagian G, Baserga SJ, Mazar A, Ferrer M, Zheng W, Shilatifard A, Aubé J, Rudloff U, Marugan JJ, Huang S (2018) Metarrestin, a perinucleolar compartment inhibitor, effectively suppresses metastasis. Sci Transl Med. 10(441):eaap8307. https://doi.org/10.1126/scitranslmed.aap8307

Article  CAS  PubMed  PubMed Central  Google Scholar 

Frankowski KJ, Patnaik S, Wang C, Southall N, Dutta D, De S, Li D, Dextras C, Lin YH, Bryant-Connah M, Davis D, Wang F, Wachsmuth LM, Shah P, Williams J, Kabir M, Zhu E, Baljinnyam B, Wang A, Xu X, Norton J, Ferrer M, Titus S, Simeonov A, Zheng W, Mathews Griner LA, Jadhav A, Aubé J, Henderson MJ, Rudloff U, Schoenen FJ, Huang S, Marugan JJ (2022) Discovery and optimization of pyrrolopyrimidine derivatives as selective disruptors of the perinucleolar compartment, a marker of tumor progression toward metastasis. J Med Chem. 65(12):8303–8331. https://doi.org/10.1021/acs.jmedchem.2c00204

Article  CAS  PubMed  PubMed Central  Google Scholar 

Frankowski K, Patnaik S, Schoenen F, Huang S, Norton J, Wang C, Titus S, Ferrer M, Zheng W, Southall N, Day VW, Aubé J, Marugan JJ. (2010) Discovery and Development of Small Molecules That Reduce PNC Prevalence. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010.

Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B (2023) Real-time imaging of RNA polymerase I activity in living human cells. J Cell Biol. 222(1):e202202110. https://doi.org/10.1083/jcb.202202110

Article  CAS  PubMed  Google Scholar 

Gagnon-Kugler T, Langlois F, Stefanovsky V, Lessard F, Moss T (2009) Loss of human ribosomal gene CpG methylation enhances cryptic RNA polymerase II transcription and disrupts ribosomal RNA processing. Mol Cell 35(4):414–425. https://doi.org/10.1016/j.molcel.2009.07.008

Article  CAS  PubMed  Google Scholar 

Garus A, Autexier C (2021) Dyskerin: an essential pseudouridine synthase with multifaceted roles in ribosome biogenesis, splicing, and telomere maintenance. RNA. 12:1441–1458. https://doi.org/10.1261/rna.078953.121

Article  CAS  Google Scholar 

Hilton J, Gelmon K, Bedard PL, Tu D, Xu H, Tinker AV, Goodwin R, Laurie SA, Jonker D, Hansen AR, Veitch ZW, Renouf DJ, Hagerman L, Lui H, Chen B, Kellar D, Li I, Lee SE, Kono T, Cheng BYC, Yap D, Lai D, Beatty S, Soong J, Pritchard KI, Soria-Bretones I, Chen E, Feilotter H, Rushton M, Seymour L, Aparicio S, Cescon DW (2022) Results of the phase I CCTG IND.231 trial of CX-5461 in patients with advanced solid tumors enriched for DNA-repair deficiencies. Nat Commun. 13(1):3607. https://doi.org/10.1038/s41467-022-31199-2

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hirose T, Ninomiya K, Nakagawa S, Yamazaki T (2023) A guide to membraneless organelles and their various roles in gene regulation. Nat Rev Mol Cell Biol 24(4):288–304. https://doi.org/10.1038/s41580-022-00558-8

Article  CAS  PubMed  Google Scholar 

Huang S, Deerinck TJ, Ellisman MH, Spector DL (1997) The dynamic organization of the perinucleolar compartment in the cell nucleus. J Cell Biol 137(5):965–974. https://doi.org/10.1083/jcb.137.5.965

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jacobs RQ, Huffines AK, Laiho M, Schneider DA (2022) The small-molecule BMH-21 directly inhibits transcription elongatio