Advances in Pharmacology and Pharmacy Vol. 12(2), pp. 104 - 115
DOI: 10.13189/app.2024.120203
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Puja Das 1, Dipanwita Mitra 2, Dibya Pal 2, Debidas Ghosh 2,*
1 Centre for Life Sciences, Vidyasagar University, India
2 Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, India

ABSTRACT

Background: Tinospora cordifolia (Willd.) Miers. traditionally familiar as giloy or gulancha has been used as a traditional folk medicine in Ayurveda from ancient times due to its antifertility effect. However, the mechanisms behind the hypo-testicular action of this herbal plant remain unclear. In this context, this investigation has been performed to unfold the probable mechanism of action by an effective dose of the H2O-methanol (60:40) extract (HME) of T. cordifolia stem. Objective: To determine the effective dose of T. cordifolia's HME for hypo-testicular efficacy testing in male albino rats. Materials and methods: Doses of T. cordifolia's HME at 5 (low), 10 (moderate), and 20 (high) mg/100 g of body weight (BW)/day for 28 days were given to the experimental rats in three separate dose-treated groups by oral gavage feeding. Rats in the vehicle-treated control group (VTCG) were fed only 0.5 ml sterile water/100 g of BW/day. Spermiological profile, seminal vesicular fructose level, androgenic markers, oxidative stress parameters, and testicular histological studies were analyzed. Results: Different doses of HME-treated groups (HMETGs) delineated a downward deviation significantly (pth stage of the spermatokinetics in respect to VTCG. Testicular thiobarbituric acid reactive substances (TBARS) and cholesterol levels were elevated significantly (p0.05). Conclusion: The observation enlightens the HME of T. cordifolia stem at the dose of 10 mg (moderate) which executes maximum hypo-testicular activities for male contraception development as well as it also highlighting the therapeutic window of HME in this concern.

KEYWORDS
Tinospora cordifolia, Hypo-Testicular Activity, Spermiological Profile, Androgenic Key Enzymes, Oxidative Stress

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Puja Das , Dipanwita Mitra , Dibya Pal , Debidas Ghosh , "Hypo-testicular Efficacy Assessment of H2O-methanol (60:40) Extract of Tinospora cordifolia (Willd.) Stem in Albino Rat: A Dose-dependent Study," Advances in Pharmacology and Pharmacy, Vol. 12, No. 2, pp. 104 - 115, 2024. DOI: 10.13189/app.2024.120203.

(b). APA Format:
Puja Das , Dipanwita Mitra , Dibya Pal , Debidas Ghosh (2024). Hypo-testicular Efficacy Assessment of H2O-methanol (60:40) Extract of Tinospora cordifolia (Willd.) Stem in Albino Rat: A Dose-dependent Study. Advances in Pharmacology and Pharmacy, 12(2), 104 - 115. DOI: 10.13189/app.2024.120203.