Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Yuan J, Yang J, Zhuang Z, Yang Y, Lin L, Wang S. Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo. BMC Biotechnol. 2012;2012(12):36.

Article  Google Scholar 

Taneja K, Bajaj BK, Kumar S, Dilbaghi N. Production, purification and characterization of fibrinolytic enzyme from Serratia sp. KG-2-1 using optimized media. 3 Biotech. 2017;7(3):184.

Article  PubMed  PubMed Central  Google Scholar 

Chung DM, Choi NS, Chun HK, Maeng PJ, Park SB, Kim SH. A new fibrinolytic enzyme (55 kDa) from Allium tuberosum: purification, characterization, and comparison. J Med Food. 2010;13:1532–6.

Article  CAS  PubMed  Google Scholar 

Huang MX, Ye Y, Chen YX, Han YL. Partial purification and characterization of fibrinolytic enzymes from yellow mealworm. Int J Pept Res Ther. 2012;18:153–61.

Article  CAS  Google Scholar 

Ju X, Cao X, Sun Y, Wang Z, Cao C, Liu J, Jiang J. Purification and characterization of a fibrinolytic enzyme from Streptomyces sp. XZNUM 00004. World J Microbiol Biotechnol. 2012;28:2479–86.

Article  CAS  PubMed  Google Scholar 

Dharmawati IGAA, Mahadewa TGB, Widyadharma IPE. Antibacterial activity of Lumbricus rubellus earthworm extract against Porphyromonas gingivalis as the bacterial cause of periodontis. Maced J Med Sci. 2019;7(6):1032–6.

Article  Google Scholar 

Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS. Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling. J Mol Cell Cardiol. 2016;99:113–22.

Article  CAS  PubMed  Google Scholar 

Samatra DPGP, Tjokorda GBM, Sukrama DMI, Dewi NYS, Praja RK, Nurmansyah D. Widyadharma IPE. Extract of earthworms (Lumbricus rubellus) reduced malondialdehyde and 8-hydroxy-deoxyguanosine level in male Wistar rats infected by Salmonella typhi. Biomed Pharmacol J. 2017;10(4):1765–71.

Article  Google Scholar 

Permana S, Hadi RP, Norahmawati E, Endharti AT. Coelomic fluid of Lumbricus rubellus enhances anti-prolioniferative effect of 5-fluorouracil by modulating focal adhesion kinase express and IL-1β of colorectal cancer in mice. J Appl Pharm Sci. 2019;9(08):041–6.

Article  CAS  Google Scholar 

Ozkan AD, Eskiler GG, Sarihan M, Kazan N, Aksoy O, Yuksel B, Betts Z. Anticancer properties of Eisenia foetida proteins in prostate cancer cells in vitro. Int J Pept Res Ther. 2022;28:119.

Article  CAS  Google Scholar 

Liu CM, Chen XT, Pan YY, Liang H, Song SL, Ji AG. Antitumor studies of earthworms fibrinolytic enzyme component A from Eisenia foetida on breast cancer cell line MCF-7. Indian J Pharm Sci. 2017;79(3):361–8.

Article  CAS  Google Scholar 

Lannuci NB, Camperi SA, Cascone O. Purification of enzymes from earthworms. Dynam Soil Dynam Plant. 2009;3(Special issue 2):64–8.

Lee CK, Shin JS, Kim BS, Cho IH, Kim YS, Lee EB. Antithrombotic effects by oral administration ofnovel proteinase from earthworm Eisenia andrei on venous thrombosis model in rats. Archives Pharmacal Res. 2007;30:475–80.

Article  CAS  Google Scholar 

Thuy LTB, Tuyen ET, Dao NTN. Purification and identification of some characters of the fibrinolytic enzyme isolated from the earthworm Perionyx excavatus. Biology Magazine (Vietnam). 2006;2006(28):77–82.

Google Scholar 

Fan Q, Wu C, Li L, et al. Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus. Biochim Biophys Acta. 2001;1526(3):286–92.

Article  CAS  PubMed  Google Scholar 

Cooper EL, Ru B, Weng N. Earthworms: sources of antimicrobial and anticancer molecules. Adv in Experiment Med Biol. 2004;546:359–89.

Article  Google Scholar 

Chen H, Takahasi S, Imamura M. Earthworm fibrinolytic enzyme: anti-tumor activity on human hepatoma cells in vitro and in vivo. Chin Med J. 2007;120:898–904.

Article  CAS  PubMed  Google Scholar 

Verma MK, Pulicherla KK. Enzyme promiscuity in earthworm serine protease: substrate versatility and therapeutic potential. Amino acid. 2016;48:941–8.

Article  CAS  Google Scholar 

Wei S, Yin X, Kou Y, Jiang B. Lumbricus extract promotes the regeneration of injured peripheral nerve in rats. J Ethnopharm. 2009;123:51–4.

Article  Google Scholar 

Trisina J, Sunardi F, Suhartono MT, Raymond R, Tjandrawinata RR. DLBS1033, a protein extract from Lumbricus rubellus, possesses antithrombotic and thrombolytic activities. J Biomed Biotechnol. 2011;2011:1–8.

Article  Google Scholar 

Tjandrawinata RR, Trisina J, Rahayu P, Prasetya LA, Hanafiah A, Rachmawaty H. Bioactive protein fraction DLBS1033 containing lumbrokinase isolated from Lumbricuss rubellus: ex vivo, in vivo, and pharmaceutic studies. Drug Des Dev Ther. 2014;8:1585–93.

Article  Google Scholar 

Gayatri A, Nafrialdi N, Setiabudy RD, Tjandrawinata RR, Rachman A, Louisa M. A Clinical trial on biological half life of bioactive protein from Lumbricus rubellus, DLBS1033 in healthy volunteers. Indones J Intern Med. 2018;50(3):208–14.

Google Scholar 

Christy G, Tjandrawinata RR, Rachmawati H. Tabletting process of pellets containing bioactive fraction DLBS1033F isolated from Lumbricus rubellus: challenge and strategy. Bio Pharm Bull. 2015;38(12):1843–9.

Article  CAS  Google Scholar 

Laemmli UK. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–5.

Article  CAS  PubMed  Google Scholar 

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-Phenol reagents. J Biol Chem. 1951;193:265–75.

Article  CAS  PubMed  Google Scholar 

Bringans S, Eriksen S, Kendrick T, Gopalakrishnakone P, Livk A, Lock R. Proteomic analyses of the venom of Heterometruslongimanus (Asian black scorpion). Proteomics. 2008;8:1081–96.

Article  CAS  PubMed  Google Scholar 

Phan TT, Ta TD, Nguyen DT, Van Den Broek LA, Duong GT. Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus. AMB Express. 2011;1(1):26.

Article  PubMed  PubMed Central  Google Scholar 

Wang F, Wang G, Li M, Cui L, Zhan J, Chang W. Purification, characterization and crystallization of a group of earthworm fibrinolytic enzymes from Eisenia foetida. Biotechnol Lett. 2003;25:1105–9.

Article  CAS  PubMed  Google Scholar 

Biron DG, Brun C, Lefevre T, Lebarbenchon C, Loxdale HD, Chevenet F, Brizard JP, Thomas F. The pitfalls of proteomics experiments without the correct use of bioinformatics tools. Proteomics. 2006;6:5577–96.

Article  CAS  PubMed  Google Scholar 

Damodaran S, Wood TD, Nagarajan P, Rabin R. Evaluating peptide mass fingerprinting-based protein identification. Geno Prot Bioinfo. 2007;5:3–4.

Article  Google Scholar 

Barret J, Brophy PM, Hamilton JV. Analysing proteomic data. Int J Parasitol. 2005;35(5):543–53.

Article  Google Scholar 

Garza S, Moini M. Analysis of complex protein mixtures with improved sequence coverage using (CE-MS/MS). Anal Chem. 2006;78(20):7309–16.

Article  CAS  PubMed  Google Scholar 

Salzano AM, Crescenzi M. Mass spectrometry to protein identification and the study of post translational modifications. Ann Ist super Sanita. 2005;41(4):443–50.

PubMed  Google Scholar 

Mirsaeidi M, Gidfar S, Schraufnagel D. Annexins family: insights into their functions and potential role in pathogenesis of sarcoidosis. J Transl Med. 2016;14:89.

Article  PubMed  PubMed Central  Google Scholar 

Tjandrawinata RR, Yunaidi DA, Susanto LW. The safety and tolerability of lumbrokinase DLBS1033 in healthy adult subjects. Drug Res. 2016;66(6):293–9.

Article  CAS  Google Scholar 

Setyopranoto I, Wibowo S, Tjandrawinata RR. Hemostasis profile and clinical outcome of acute ischemic stroke patients treated with oral lumbrokinase DLBS1033: a comparative study versus aspirin and clopidogrel. Asian J Pharm Clin Res. 2016;9(1):171–7.

Google Scholar 

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