Figure 1. Differential proteins at all time points in the control and pregnant groups (A); biological processes at all time points in the two groups (B); IPA classical biological pathways at all time points in both groups (C). C: control group; P:pregnant group.

Figure 1. Differential proteins at all time points in the control and pregnant groups (A); biological processes at all time points in the two groups (B); IPA classical biological pathways at all time points in both groups (C). C: control group; P:pregnant group.

Figure 2. Venn diagram of differential proteins in the pregnant group.

Figure 2. Venn diagram of differential proteins in the pregnant group.

Figure 3. Biological pathways of differential proteins on GD 1 d, GD 4 d, and GD 7 d by analyzed GO.

Figure 3. Biological pathways of differential proteins on GD 1 d, GD 4 d, and GD 7 d by analyzed GO.

Figure 4. Fetal rat heart development. The above is the reported heart development process. The following are cardiac developmental processes enriched by changing urinary proteomes.

Figure 4. Fetal rat heart development. The above is the reported heart development process. The following are cardiac developmental processes enriched by changing urinary proteomes.

Figure 5. Fetal rat pancreas development. The above is the reported pancreatic development process. The following are pancreatic developmental processes enriched by changing urinary proteomes.

Figure 5. Fetal rat pancreas development. The above is the reported pancreatic development process. The following are pancreatic developmental processes enriched by changing urinary proteomes.

Figure 6. Fetal rat lung development. The above is the reported lung development process. The following are lung developmental processes enriched by changing urinary proteomes.

Figure 6. Fetal rat lung development. The above is the reported lung development process. The following are lung developmental processes enriched by changing urinary proteomes.

Figure 7. Changes in pathways related to organ development over time.

Figure 7. Changes in pathways related to organ development over time.

Figure 8. (A) Biological processes by IPA (p value < 0.05) at GD 20 d. (B) Changes in coagulation-related pathways at GD 14 d, GD 18 d, and GD 20 d.

Figure 8. (A) Biological processes by IPA (p value < 0.05) at GD 20 d. (B) Changes in coagulation-related pathways at GD 14 d, GD 18 d, and GD 20 d.

Figure 9. The urinary proteome exhibited coagulation-related changes before labor. (A) The line plot for the relative abundance of coagulation-related protein MOR8A3 during pregnancy. (B) The line plot for the relative abundance of coagulation-related protein Q7TQ70 during pregnancy. Each group (time point) was involved in three experimental replicates. Relative protein expression levels at each time point were assigned the mean ± SD value. (C) Coagulation-related proteins MOR8A3, Q7TQ70, and Q8K3U6 on GD 14 d, GD 16 d, and GD 20 d.

Figure 9. The urinary proteome exhibited coagulation-related changes before labor. (A) The line plot for the relative abundance of coagulation-related protein MOR8A3 during pregnancy. (B) The line plot for the relative abundance of coagulation-related protein Q7TQ70 during pregnancy. Each group (time point) was involved in three experimental replicates. Relative protein expression levels at each time point were assigned the mean ± SD value. (C) Coagulation-related proteins MOR8A3, Q7TQ70, and Q8K3U6 on GD 14 d, GD 16 d, and GD 20 d.

Table 1. Urine proteome quantitative results.

Table 1. Urine proteome quantitative results.

BatchTime PointNumber of Identified ProteinsNumber of Proteins after Data PreprocessingFirst batchPregnant groupGD 1 d, GD 14, GD 16 d, GD 18 d, GD 20 d1703 ± 2771199Second batchPregnant groupGD 1 d, GD 4 d, GD 7 d, GD 9 d, GD 11 d1498.6 ± 2491289 Control group0 d, 4 d, 7 d, 9 d, 11 d, 14 d, 16 d, 18 d