Overexpression of NEK2 is correlated with poor prognosis in human clear cell renal cell carcinoma

Objectives: Never in mitosis gene A-related kinase 2 (NEK2) has been implicated in tumorigenesis in various tissues, but its function in clear cell renal cell carcinoma (ccRCC) tumorigenesis is unclear. We evaluated the correlation between NEK2 expression and ccRCC. Methods: Immunohistochemistry analysis of NEK2 protein was done on high-density multi-organ Human Cancer tissue microarray derived from the patient samples from clear cell renal cell carcinoma. We used multiple clinical cohorts to analyze the NEK2 immunohistochemical staining expression across human cancers. The cancer genome atlas (TCGA) data analysis of NEK2 was done through UALCAN web servers. Association of NEK2 and Kaplan–Meier survival analysis was done on both of our clinical database and available TCGA datasets. Results: Using the UALCAN cancer transcriptional data analysis website, we found that NEK2 is overexpressed in ccRCC, and its expression was associated with overall survival. According to the analyses of our own clinical database and immunohistochemical staining, protein levels of NEK2 were elevated in renal carcinoma compared to adjacent normal tissues. Kaplan–Meier survival analysis of both UALCAN and our database showed that high expression of NEK2 was associated with a poor prognosis. Multivariate and univariate analyses showed that NEK2 expression was closely related to a poor prognosis. The findings suggest that NEK2 is associated with ccRCC. Conclusion: These studies show that NEK2 is over-expressed in clear cell renal cell carcinoma and plays an essential role in cancer cell survival, as such NEK2 could serve as a novel potential target for therapeutic intervention in ccRCC.

1. Luo, J, Luo, X, Liu, X, et al. (2020) DUSP9 suppresses proliferation and migration of clear cell renal cell carcinoma via the mTOR pathway. Onco Targets Ther 13: 1321–1330. 2020/02/28. DOI:
10.2147/OTT.S239407.
Google Scholar | Crossref | Medline2. Wang, J, Tang, C, Yang, C, et al. (2019) Tropomyosin-1 functions as a tumor suppressor with respect to cell proliferation, angiogenesis and metastasis in renal cell carcinoma. J Cancer 10: 2220–2228. 2019/07/02. DOI: 10.7150/jca.28261.
Google Scholar | Crossref | Medline3. Spurlock, J (1970) Social deprivation in childhood and character formation. J Am Psychoanal Assoc 18: 622–630. 1970/07/01. DOI: 10.1177/000306517001800305.
Google Scholar | SAGE Journals | ISI4. Clark, EA, Draves, KE (1987) Activation of macaque T cells and B cells with agonistic monoclonal antibodies. Eur J Immunol 17: 1799–1805. 1987/12/01. DOI: 10.1002/eji.1830171219.
Google Scholar | Crossref | Medline5. Fang, Y, Zhang, X (2016) Targeting NEK2 as a promising therapeutic approach for cancer treatment. Cell Cycle 15: 895–907. 2016/03/29. DOI: 10.1080/15384101.2016.1152430.
Google Scholar | Crossref | Medline6. Hayward, DG, Fry, AM (2006) Nek2 kinase in chromosome instability and cancer. Cancer Lett 237: 155–166. 2005/08/09. DOI: 10.1016/j.canlet.2005.06.017.
Google Scholar | Crossref | Medline7. Marina, M, Saavedra, HI (2014) Nek2 and Plk4: prognostic markers, drivers of breast tumorigenesis and drug resistance. Front Biosci (Landmark Ed) 19: 352–365. 2014/01/07. DOI: 10.2741/4212.
Google Scholar | Crossref | Medline8. Oliveira, A, Issayama, L, Pavan, I, et al. (2020) Checking NEKs: Overcoming a bottleneck in human diseases. Molecules 25: 1778.
Google Scholar | Crossref9. Zhou, L, Ding, L, Gong, Y, et al. (2021) NEK2 promotes cell proliferation and glycolysis by regulating PKM2 abundance via phosphorylation in diffuse large B-cell lymphoma. Front Oncol 11: 677763. 2021/06/26. DOI: 10.3389/fonc.2021.677763.
Google Scholar | Crossref | Medline10. Bai, R, Yuan, C, Sun, W, et al. (2021) NEK2 plays an active role in tumorigenesis and tumor microenvironment in non-small cell lung cancer. Int J Biol Sci 17: 1995–2008. 2021/06/17. DOI: 10.7150/ijbs.59019.
Google Scholar | Crossref | Medline11. Wan, H, Xu, L, Zhang, H, et al. (2021) High expression of NEK2 promotes gastric cancer progression via activating AKT signaling. J Physiol Biochem 77: 25–34. 2020/11/18. DOI: 10.1007/s13105-020-00776-8.
Google Scholar | Crossref | Medline

留言 (0)

沒有登入
gif