Front. Immunol.

Sec. Immunological Tolerance and Regulation

Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1497783

Provisionally accepted

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Background: Our previous research highlighted the potential role of immunoglobulin G4 (IgG4) in mediating immunosuppression within the tumor microenvironment (TME). Tertiary lymphoid structures (TLS) in the TME have important immune-related functions. This study aims to analyze the distribution characteristics of IgG4-expressing cells, regulatory T cells (Tregs), and M2-type macrophages as well as to elucidate the relationship between IgG4 and the polarization of M2 macrophages within TLS in esophageal cancer. Object: To elucidate the distribution of IgG4, Treg cells, and M2 macrophages in TLS and to assess the impact of IgG4 on macrophage polarization. Methods: Esophageal cancer tissue were analyzed with multiplex immunofluorescence to determine the spatial distribution and density of B cells, T cells, and their subtypes. The relationship between IgG4 and CD8+ T cells in TLS, along with interleukin-10 (IL-10) expression and Treg presence, was studied. Serum IgG4 and IL-10 levels were compared between patients and healthy controls. In vitro, the impact of IgG4 on monocyte differentiation into M2 macrophages was observed. Results: IgG4 density was inversely related with CD8+ T cells in mature TLS tumor microenvironment 删除[王大海]: 字体: (默认)Times New Roman Italic, 倾斜 设置格式[王大海]: indicating a potential immunosuppressive role (P

Keywords: Immunoglobulin G4, tertiary lymphoid structure, Tumor Microenvironment, Macrophage polarization, Interleukin-10

Received: 17 Sep 2024; Accepted: 30 Dec 2024.

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