Increasing carotenoid production in Xanthophyllomyces dendrorhous/Phaffia rhodozyma: SREBP pathway activation and promoter engineering

Higuera-Ciapara I, Félix-Valenzuela L, Goycoolea FM. Astaxanthin: a review of its chemistry and applications. Crit Rev Food Sci Nutr. 2006;46:185–96.

Article  CAS  PubMed  Google Scholar 

Galasso C, Corinaldesi C, Sansone C. Carotenoids from marine organisms: biological functions and industrial applications. Antioxidants. 2017;6:96.

Article  PubMed  PubMed Central  Google Scholar 

Johnson EA. Phaffia rhodozyma: colorful odyssey. Int Microbiol. 2003;6:169–74.

Article  CAS  PubMed  Google Scholar 

Schmidt I, Schewe H, Gassel S, Jin C, Buckingham J, Hümbelin M, Sandmann G, Schrader J. Biotechnological production of astaxanthin with Phaffia rhodozyma/Xanthophyllomyces dendrorhous. Appl Microbiol Biotechnol. 2011;89:555–71.

Article  CAS  PubMed  Google Scholar 

Rodriguez-Saiz M, de la Fuente JL, Barredo JL. Xanthophyllomyces dendrorhous for the industrial production of astaxanthin. Appl Microbiol Biotechnol. 2010;88:645–58.

Article  CAS  PubMed  Google Scholar 

Mata-Gomez LC, Montanez JC, Mendez-Zavala A, Aguilar CN. Biotechnological production of carotenoids by yeasts: an overview. Microb Cell Fact. 2014;13:12.

Article  PubMed  PubMed Central  Google Scholar 

Torres-Haro A, Verdín J, Kirchmayr MR, Arellano-Plaza M. Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous. Microb Cell Fact. 2021;20:175.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Breitenbach J, Visser H, Verdoes JC, van Ooyen AJ, Sandmann G. Engineering of geranylgeranyl pyrophosphate synthase levels and physiological conditions for enhanced carotenoid and astaxanthin synthesis in Xanthophyllomyces dendrorhous. Biotechnol Lett. 2010;33:755–61.

Article  PubMed  Google Scholar 

Alcaíno J, Romero I, Niklitschek M, Sepúlveda D, Rojas MC, Baeza M, Cifuentes V. Functional characterization of the Xanthophyllomyces dendrorhous farnesyl pyrophosphate synthase and geranylgeranyl pyrophosphate synthase encoding genes that are involved in the synthesis of isoprenoid precursors. PLoS ONE. 2014;9: e96626.

Article  PubMed  PubMed Central  Google Scholar 

Bien C, Espenshade P. Sterol regulatory element binding proteins in fungi: hypoxic transcription factors linked to pathogenesis. Eukaryot Cell. 2010;9:352–9.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Goldstein JL, DeBose-Boyd RA, Brown MS. Protein sensors for membrane sterols. Cell. 2006;124:35–46.

Article  CAS  PubMed  Google Scholar 

Moon YA, Liang G, Xie X, Frank-Kamenetsky M, Fitzgerald K, Koteliansky V, Brown MS, Goldstein JL, Horton JD. The Scap/SREBP pathway is essential for developing diabetic fatty liver and carbohydrate-induced hypertriglyceridemia in animals. Cell Metab. 2012;15:240–6.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wu X, Yan R, Cao P, Qian H, Yan N. Structural advances in sterol-sensing domain-containing proteins. Trends Biochem Sci. 2022;47:289–300.

Article  CAS  PubMed  Google Scholar 

Brown MS, Goldstein JL. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood. Proc Natl Acad Sci U S A. 1999;96:11041–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rawson RB. Control of lipid metabolism by regulated intramembrane proteolysis of sterol regulatory element binding proteins (SREBPs). Biochem Soc Symp. 2003;70:221–31.

Article  CAS  Google Scholar 

Osborne TF, Espenshade PJ. Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it’s been. Genes Dev. 2009;23:2578–91.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hughes AL, Todd BL, Espenshade PJ. SREBP pathway responds to sterols and functions as an oxygen sensor in fission yeast. Cell. 2005;120:831–42.

Article  CAS  PubMed  Google Scholar 

Chun CD, Liu OW, Madhani HD. A link between virulence and homeostatic responses to hypoxia during infection by the human fungal pathogen Cryptococcus neoformans. PLoS Pathog. 2007;3: e22.

Article  PubMed  PubMed Central  Google Scholar 

Chang YC, Bien CM, Lee H, Espenshade PJ, Kwon-Chung KJ. Sre1p, a regulator of oxygen sensing and sterol homeostasis, is required for virulence in Cryptococcus neoformans. Mol Microbiol. 2007;64:614–29.

Article  CAS  PubMed  Google Scholar 

Gómez M, Campusano S, Gutiérrez MS, Sepúlveda D, Barahona S, Baeza M, Cifuentes V, Alcaíno J. Sterol regulatory element-binding protein Sre1 regulates carotenogenesis in the red yeast Xanthophyllomyces dendrorhous. J Lipid Res. 2020;61:1658–74.

Article  PubMed  PubMed Central  Google Scholar 

Gómez M, Baeza M, Cifuentes V, Alcaíno J. The SREBP (sterol regulatory element-binding protein) pathway: a regulatory bridge between carotenogenesis and sterol biosynthesis in the carotenogenic yeast Xanthophyllomyces dendrorhous. Biol Res. 2021;54:34.

Article  PubMed  PubMed Central  Google Scholar 

Gutiérrez MS, Campusano S, González AM, Gómez M, Barahona S, Sepúlveda D, Espenshade PJ, Fernández-Lobato M, Baeza M, Cifuentes V, Alcaíno J. Sterol regulatory element-binding protein (Sre1) promotes the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous. Front Microbiol. 2019;10:586.

Article  PubMed  PubMed Central  Google Scholar 

Gómez M, Gutiérrez MS, González AM, Gárate-Castro C, Sepúlveda D, Barahona S, Baeza M, Cifuentes V, Alcaíno J. Metallopeptidase Stp1 activates the transcription factor Sre1 in the carotenogenic yeast Xanthophyllomyces dendrorhous. J Lipid Res. 2020;61:229–43.

Article  PubMed  Google Scholar 

Loto I, Gutiérrez MS, Barahona S, Sepúlveda D, Martínez-Moya P, Baeza M, Cifuentes V, Alcaíno J. Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous. BMC Microbiol. 2012;12:235.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Adrio JL, Veiga M. Transformation of the astaxanthin-producing yeast Phaffia rhodozyma. Biotechnol Tech. 1995;9:509–12.

Article  CAS  Google Scholar 

Kim IG, Nam SK, Sohn JH, Rhee SK, An GH, Lee SH, Choi ES. Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use a drug resistance marker for transformation. Appl Environ Microbiol. 1998;64:1947–9.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods. 2001;25:402–8.

Article  CAS  PubMed  Google Scholar 

Boyle JS, Lew AM. An inexpensive alternative to glassmilk for DNA purification. Trends Genet. 1995;11:8.

Article  CAS  PubMed  Google Scholar 

Sambrook J, Russell DW. Molecular cloning: a laboratory manual. 3rd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 2001.

Google Scholar 

Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156–9.

Article  CAS  PubMed  Google Scholar 

An G-H, Schuman DB, Johnson EA. Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl Environ Microbiol. 1989;55:116–24.

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