Friedenstein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 1987;20:263–72.

PubMed  CAS  Google Scholar 

Pittenger MF, Discher DE, Péault BM, Phinney DG, Hare JM, Caplan AI. Mesenchymal stem cell perspective: cell biology to clinical progress. NPJ Regen Med. 2019;4:22.

Article  PubMed  PubMed Central  Google Scholar 

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. the international society for cellular therapy position statement. Cytotherapy. 2006;8:315–7.

Article  PubMed  CAS  Google Scholar 

Viswanathan S, Shi Y, Galipeau J, Krampera M, Leblanc K, Martin I, et al. Mesenchymal stem versus stromal cells: international society for cell & gene therapy (ISCT®) mesenchymal stromal cell committee position statement on nomenclature. Cytotherapy. 2019;21:1019–24.

Article  PubMed  CAS  Google Scholar 

Pinho AG, Cibrão JR, Silva NA, Monteiro S, Salgado AJ. Cell secretome: basic insights and therapeutic opportunities for CNS disorders. Pharmaceuticals. 2020;13:31.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Teixeira FG, Carvalho MM, Panchalingam KM, Rodrigues AJ, Mendes-Pinheiro B, Anjo S, et al. Impact of the secretome of human mesenchymal stem cells on brain structure and animal behavior in a rat model of Parkinson’s disease. Stem Cells Transl Med. 2017;6:634–46.

Article  PubMed  CAS  Google Scholar 

Teixeira FG, Vilaça-Faria H, Domingues VA, Campos J, Salgado AJ. Preclinical comparison of stem cells secretome and levodopa application in a 6-Hydroxydopamine rat model of Parkinson’s disease. Cells. 2020;9:315.

Article  Google Scholar 

Vilaça-Faria H, Marote A, Lages I, Ribeiro C, Mendes-Pinheiro B, Domingues AV, et al. Fractionating stem cells secretome for Parkinson’s disease modeling: Is it the whole better than the sum of its parts? Biochimie. 2021;189:87–98.

Article  PubMed  Google Scholar 

Mendes-Pinheiro B, Campos J, Marote A, Soares-Cunha C, Nickels SL, Monzel AS, et al. Treating parkinson’s disease with human bone marrow mesenchymal stem cell secretome: a translational investigation using human brain organoids and different routes of in vivo administration. Cells. 2023;12:2565.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Pinho AG, Cibrão JR, Lima R, Gomes ED, Serra SC, Lentilhas-Graça J, et al. Immunomodulatory and regenerative effects of the full and fractioned adipose tissue derived stem cells secretome in spinal cord injury. Exp Neurol. 2022;351:113989.

Article  PubMed  CAS  Google Scholar 

Assunção-Silva R, Pinho A, Cibrão JC, Salgado A. Adipose tissue derived stem cell secretome induces motor and histological gains after complete spinal cord injury in Xenopus laevis and Mice. J Tissue Eng. 2024;15:20417314231203824.

Article  PubMed  PubMed Central  Google Scholar 

Miceli V, Zito G, Bulati M, Gallo A, Busà R, Iannolo G, et al. Different priming strategies improve distinct therapeutic capabilities of mesenchymal stromal/stem cells: Potential implications for their clinical use. World J Stem Cells. 2023;15:400–20.

Article  PubMed  PubMed Central  Google Scholar 

Robb KP, Galipeau J, Shi Y, Schuster M, Martin I, Viswanathan S. Failure to launch commercially-approved mesenchymal stromal cell therapies: what's the path forward? In: Proceedings of the International Society for Cell & Gene Therapy (ISCT) Annual Meeting Roundtable held in May 2023, Palais des Congrès de Paris, Organized by the ISCT MSC Scientific Committee. Cytotherapy. 2024;26:413–7.

Noronha NdC, Mizukami A, Caliári-Oliveira C, Cominal JG, Rocha JLM, Covas DT, et al. Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies. Stem Cell Res Ther. 2019;10:131.

Article  PubMed  Google Scholar 

Teixeira FG, Panchalingam KM, Anjo SI, Manadas B, Pereira R, Sousa N, et al. Do hypoxia/normoxia culturing conditions change the neuroregulatory profile of Wharton Jelly mesenchymal stem cell secretome? Stem Cell Res Ther. 2015;6:133.

Article  PubMed  PubMed Central  Google Scholar 

Marques CR, Fuzeta MdA, dos Santos Cunha RM, Pereira-Sousa J, Silva D, Campos J, et al. Neurodifferentiation and neuroprotection potential of mesenchymal stromal cell-derived secretome produced in different dynamic systems. Biomedicines. 2023;11:1240.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Tang W, Zeve D, Suh JM, Bosnakovski D, Kyba M, Hammer RE, et al. White fat progenitor cells reside in the adipose vasculature. Science. 2008;322:583–6.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Scamfer SR, Lee MD, Hilgendorf KI. Ciliary control of adipocyte progenitor cell fate regulates energy storage. Front Cell Dev Biol. 2022;10:1083372.

Article  PubMed  PubMed Central  Google Scholar 

Hilgendorf KI, Johnson CT, Mezger A, Rice SL, Norris AM, Demeter J, et al. Omega-3 fatty acids activate ciliary FFAR4 to control adipogenesis. Cell. 2019;179:1289-305.e21.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Jiang Y, Berry DC, Jo A, Tang W, Arpke RW, Kyba M, et al. A PPARγ transcriptional cascade directs adipose progenitor cell-niche interaction and niche expansion. Nat Commun. 2017;8:15926.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Oster RT, Tishinsky JM, Yuan Z, Robinson LE. Docosahexaenoic acid increases cellular adiponectin mRNA and secreted adiponectin protein, as well as PPARγ mRNA, in 3T3-L1 adipocytes. Appl Physiol Nutr Metab. 2010;35:783–9.

Article  PubMed  CAS  Google Scholar 

Silva NA, Moreira J, Ribeiro-Samy S, Gomes ED, Tam RY, Shoichet MS, et al. Modulation of bone marrow mesenchymal stem cell secretome by ECM-like hydrogels. Biochimie. 2013;95:2314–9.

Article  PubMed  CAS  Google Scholar 

Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676–82.

Article  PubMed  CAS  Google Scholar 

Marote A, Santos D, Mendes-Pinheiro B, Serre-Miranda C, Anjo SI, Vieira J, et al. Cellular Aging Secretes: a Comparison of Bone-Marrow-Derived and Induced Mesenchymal Stem Cells and Their Secretome Over Long-Term Culture. Stem Cell Rev and Rep. 2023;19:248–63.

Article  PubMed  CAS  Google Scholar 

Balachandra S, Sarkar S, Amodeo AA. The nuclear-to-cytoplasmic ratio: coupling DNA content to cell size, cell cycle, and biosynthetic capacity. Annu Rev Genet. 2022;56:165–85.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Abdolmohammadi K, Mahmoudi T, Nojehdehi S, Tayebi L, Hashemi SM, Noorbakhsh F, et al. Effect of hypoxia preconditioned adipose-derived mesenchymal stem cell conditioned medium on cerulein-induced acute pancreatitis in mice. Adv Pharm Bull. 2020;10:297–306.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Mark P, Kleinsorge M, Gaebel R, Lux CA, Toelk A, Pittermann E, et al. Human mesenchymal stem cells display reduced expression of cd105 after culture in serum-free medium. Stem Cells Int. 2013;2013:698076.

Article  PubMed  PubMed Central  Google Scholar 

Li S-N, Wu J-F. TGF-β/SMAD signaling regulation of mesenchymal stem cells in adipocyte commitment. Stem Cell Res Ther. 2020;11:41.

Article  PubMed  PubMed Central  Google Scholar 

Pinho TS, Cibrão JR, Silva D, Barata-Antunes S, Campos J, Afonso JL, et al. In vitro neuronal and glial response to magnetically stimulated piezoelectric poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV)/cobalt ferrite (CFO) microspheres. Biomater Adv. 2024;159: 213798.

Article  PubMed  CAS  Google Scholar 

Marques CR, Campos J, Sampaio-Marques B, Antunes FF, Cunha RMdS, Silva D, et al. Secretome of bone marrow mesenchymal stromal cells cultured in a dynamic system induces neuroprotection and modulates microglial responsiveness in an α-synuclein overexpression rat model. Cytotherapy. 2024;26:700–13.

Article  PubMed  CAS