Pankratz D, Gomez NO, Nielsen A, Mustafayeva A, Gür M, Arce-Rodriguez F, et al. An expanded CRISPR-Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates. Nat Protoc. 2023;18(11):3253–88.

Article  CAS  PubMed  Google Scholar 

Wang M, Chen M, Wu X, Huang X, Yu B. CRISPR applications in cancer diagnosis and treatment. Cell Mol Biol Lett. 2023;28(1):73.

Article  PubMed  PubMed Central  Google Scholar 

Patel SJ, Sanjana NE, Kishton RJ, Eidizadeh A, Vodnala SK, Cam M, et al. Identification of essential genes for cancer immunotherapy. Nature. 2017;548(7669):537–42.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, et al. Identification and characterization of essential genes in the human genome. Science. 2015;350(6264):1096–101.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Vaghari-Tabari M, Hassanpour P, Sadeghsoltani F, Malakoti F, Alemi F, Qujeq D, et al. CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer. Cell Mol Biol Lett. 2022;27(1):49.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators of immune function. Cell. 2018;175(7):1958-71 e15.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Reina-Campos M, Heeg M, Kennewick K, Mathews IT, Galletti G, Luna V, et al. Metabolic programs of T cell tissue residency empower tumour immunity. Nature. 2023;621(7977):179–87.

Article  CAS  PubMed  Google Scholar 

Chen Z, Arai E, Khan O, Zhang Z, Ngiow SF, He Y, et al. In vivo CD8(+) T cell CRISPR screening reveals control by Fli1 in infection and cancer. Cell. 2021;184(5):1262-80 e22.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Villa M, Stanczak MA, Pearce EL. How to make a better T cell: in vivo CRISPR screens have some answers. Cell. 2021;184(5):1135–6.

Article  CAS  PubMed  Google Scholar 

West EE, Merle NS, Kaminski MM, Palacios G, Kumar D, Wang L, et al. Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection. Immunity. 2023;56(9):2036-53 e12.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Heczey A, Liu D, Tian G, Courtney AN, Wei J, Marinova E, et al. Invariant NKT cells with chimeric antigen receptor provide a novel platform for safe and effective cancer immunotherapy. Blood. 2014;124(18):2824–33.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sutra Del Galy A, Menegatti S, Fuentealba J, Lucibello F, Perrin L, Helft J, et al. In vivo genome-wide CRISPR screens identify SOCS1 as intrinsic checkpoint of CD4(+) T(H)1 cell response. Sci Immunol. 2021;6(66): eabe8219.

Article  PubMed  Google Scholar 

Cecil D, Park KH, Curtis B, Corulli L, Disis MN. Type I T cells sensitize treatment refractory tumors to chemotherapy through inhibition of oncogenic signaling pathways. J Immunother Cancer. 2021;9(3).

Schank M, Zhao J, Wang L, Nguyen LNT, Zhang Y, Wu XY, et al. ROS-induced mitochondrial dysfunction in CD4 T cells from ART-controlled people living with HIV. Viruses. 2023;15(5):1061.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ferreira ACF, Szeto ACH, Clark PA, Crisp A, Kozik P, Jolin HE, et al. Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation. Immunity. 2023;56(7):1468-84 e7.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Szeto ACH, Ferreira ACF, Mannion J, Clark PA, Sivasubramaniam M, Heycock MWD, et al. An alphavbeta3 integrin checkpoint is critical for efficient T(H)2 cell cytokine polarization and potentiation of antigen-specific immunity. Nat Immunol. 2023;24(1):123–35.

Article  CAS  PubMed  Google Scholar 

Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, et al. Genome-wide CRISPR screens in T helper cells reveal pervasive crosstalk between activation and differentiation. Cell. 2019;176(4):882-96e18.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bapat SP, Whitty C, Mowery CT, Liang Y, Yoo A, Jiang Z, et al. Obesity alters pathology and treatment response in inflammatory disease. Nature. 2022;604(7905):337–42.

Article  CAS  PubMed  PubMed Central  Google Scholar 

MacMillan ML, Hippen KL, McKenna DH, Kadidlo D, Sumstad D, DeFor TE, et al. First-in-human phase 1 trial of induced regulatory T cells for graft-versus-host disease prophylaxis in HLA-matched siblings. Blood Adv. 2021;5(5):1425–36.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang W, Chen Y, Zhao Z, Zheng H, Wang S, Liao Z, et al. Adoptive T(reg) therapy with metabolic intervention via perforated microneedles ameliorates psoriasis syndrome. Sci Adv. 2023;9(20): eadg6007.

Article  CAS  PubMed  Google Scholar 

Li C, Wang G, Sivasami P, Ramirez RN, Zhang Y, Benoist C, et al. Interferon-alpha-producing plasmacytoid dendritic cells drive the loss of adipose tissue regulatory T cells during obesity. Cell Metab. 2021;33(8):1610-23e5.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Miao Y, Zhang C, Yang L, Zeng X, Hu Y, Xue X, et al. The activation of PPARgamma enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TbetaRII/IL-2Ralpha. Cell Commun Signal. 2022;20(1):48.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Thiolat A, Pilon C, Caudana P, Moatti A, To NH, Sedlik C, et al. Treg-targeted IL-2/anti-IL-2 complex controls graft-versus-host disease and supports anti-tumor effect in allogeneic hematopoietic stem cell transplantation. Haematologica. 2024;109(1):129–42.

PubMed  Google Scholar 

Zhai N, Liu W, Jin CH, Ding Y, Sun L, Zhang D, et al. Lack of IFN-gamma receptor signaling inhibits graft-versus-host disease by potentiating regulatory T cell expansion and conversion. J Immunol. 2023;211(5):885–94.

Article  CAS  PubMed  Google Scholar 

Scherlinger M, Pan W, Hisada R, Boulougoura A, Yoshida N, Vukelic M, et al. Phosphofructokinase P fine-tunes T regulatory cell metabolism, function, and stability in systemic autoimmunity. Sci Adv. 2022;8(48): eadc9657.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sugiura A, Andrejeva G, Voss K, Heintzman DR, Xu X, Madden MZ, et al. MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function. Immunity. 2022;55(1):65-81e9.

Article  CAS  PubMed  Google Scholar 

Freuchet A, Salama A, Bezie S, Tesson L, Remy S, Humeau R, et al. IL-34 deficiency impairs FOXP3(+) Treg function in a model of autoimmune colitis and decreases immune tolerance homeostasis. Clin Transl Med. 2022;12(8): e988.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Vaikunthanathan T, Landmann E, Correa DM, Romano M, Trevelin SC, Peng Q, et al. Dysregulated anti-oxidant signalling and compromised mitochondrial integrity negatively influence regulatory T cell function and viability in liver disease. EBioMedicine. 2023;95(2352–3964): 104778.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Watson MJ, Vignali PDA, Mullett SJ, Overacre-Delgoffe AE, Peralta RM, Grebinoski S, et al. Metabolic support of tumour-infiltrating regulatory T cells by lactic acid. Nature. 2021;591(7851):645–51.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kumagai S, Koyama S, Itahashi K, Tanegashima T, Lin YT, Togashi Y, et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments. Cancer Cell. 2022;40(2):201-18e9.

Article  CAS  PubMed  Google Scholar 

Shan F, Cillo AR, Cardello C, Yuan DY, Kunning SR, Cui J, et al. Integrated BATF transcriptional network regulates suppressive intratumoral regulatory T cells. Sci Immunol. 2023;8(87): eadf6717.

Article  CAS  PubMed  Google Scholar 

Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, et al. Functional CRISPR dissection of gene networks controlling human regulatory T cell identity. Nat Immunol. 2020;21(11):1456–66.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Spanier JA, Fung V, Wardell CM, Alkhatib MH, Chen Y, Swanson LA, et al. Tregs with an MHC class II peptide-specific chimeric antigen receptor prevent autoimmune diabetes in mice. J Clin Invest. 2023;133(18):78.

Google Scholar 

Cook PJ, Yang SJ, Uenishi GI, Grimm A, West SE, Wang LJ, et al. A chemically inducible IL-2 receptor signaling complex allows for effective in vitro and in vivo selection of engineered CD4+ T cells. Mol Ther. 2023;31(8):2472–88.

Article  CAS  PubMed