Expression levels of activated inflammasome components, pro-inflammatory cytokine maturation and markers of cell death in protoplasmic and fibrous astrocytes under ischemic conditions over time (A–H). Representative immunoblots and quantifications illustrating protein expression levels of activated effector (Cleaved Caspase-1, -8, and -11) inflammasome components, and downstream effector targets such as both mature IL-1β and IL-18 in lysates of protoplasmic astrocytes at indicated time points (1, 3, 6, 9, 12, and 24 h) during oxygen and glucose deprivation (OGD) conditions (A and B). Representative immunoblots and quantifications illustrating protein expression levels of activated effector (Cleaved Caspase-1, -8, and -11) inflammasome components, and downstream effector targets such as both mature IL-1β and IL-18 in lysates of fibrous astrocytes at indicated time points (1, 3, 6, 9, and 12 h) during oxygen and glucose deprivation (OGD) conditions (C and D). Representative immunoblots and quantifications illustrating protein expression levels of programmed cell death effectors such as Cleaved Caspase-3 (apoptosis), N-Terminal Gasdermin D (pyroptosis), and N-Terminal Gasdermin E (secondary pyroptosis) in lysates of protoplasmic astrocytes at indicated time points (1, 3, 6, 9, 12 and 24 h) during oxygen and glucose deprivation (OGD) conditions (E and F). Representative immunoblots and quantifications illustrating protein expression levels of programmed cell death effectors such as Cleaved Caspase-3 (apoptosis), N-Terminal Gasdermin D (pyroptosis), and N-Terminal Gasdermin E (secondary pyroptosis) in lysates of fibrous astrocytes at indicated time points (1, 3, 6, 9, and 12 h) during oxygen and glucose deprivation (OGD) conditions (G and H). β-actin was used as a loading control. Data are represented as mean ± S.E.M. n = 4–5 biological cultures in each experimental group. *p < 0.05 compared with control; **p < 0.01 compared with control; ***p < 0.001 compared with control; ****p < 0.0001 compared with control