R.A. Flynn, K. Pedram, S.A. Malaker, P.J. Batista, B.A.H. Smith, A.G. Johnson, B.M. George, K. Majzoub, P.W. Villalta, J.E. Carette, C.R. Bertozzi, Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 184, 3109-3124.e3122 (2021)

Article  CAS  Google Scholar 

G.A. Khoury, R.C. Baliban, C.A. Floudas, Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database. Sci. Rep 1, 90 (2011)

Article  CAS  Google Scholar 

A. Varki, R.D. Cummings, J.D. Esko, P. Stanley, G.W. Hart, M. Aebi, D. Mohnen, T. Kinoshita, N.H. Packer, J.H. Prestegard, R.L. Schnaar, P.H. Seeberger, Essentials of glycobiology, 4th edn. (Cold Spring Harbor, New York, 2022), pp. 103–115

R.D. Cummings, I.S. Trowbridge, S. Kornfeld, A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase. J. Biol. Chem 257, 13421–13427 (1982)

Article  CAS  Google Scholar 

Y. Lu, W. Chaney, Induction of N-acetylglucosaminyltransferase V by elevated expression of activated or proto-Ha-ras oncogenes. Mol. Cell. Biochem 122, 85–92 (1993)

Article  CAS  Google Scholar 

H. Saito, J. Gu, A. Nishikawa, Y. Ihara, J. Fujii, Y. Kohgo, N. Taniguchi, Organization of the human N-acetylglucosaminyltransferase V gene. Eur. J. Biochem 233, 18–26 (1995)

Article  CAS  Google Scholar 

R. Kang, H. Saito, Y. Ihara, E. Miyoshi, N. Koyama, Y. Sheng, N. Taniguchi, Transcriptional regulation of the N-acetylglucosaminyltransferase V gene in human bile duct carcinoma cells (HuCC-T1) is mediated by Ets-1. J. Biol. Chem 271, 26706–26712 (1996)

Article  CAS  Google Scholar 

J.H. Ko, E. Miyoshi, K. Noda, A. Ekuni, R. Kang, Y. Ikeda, N. Taniguchi, Regulation of the GnT-V promoter by transcription factor Ets-1 in various cancer cell lines. J. Biol. Chem 274, 22941–22948 (1999)

Article  CAS  Google Scholar 

P. Buckhaults, L. Chen, N. Fregien, M. Pierce, Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. J. Biol. Chem 272, 19575–19581 (1997)

Article  CAS  Google Scholar 

G. Greville, E. Llop, J. Howard, S.F. Madden, A.S. Perry, R. Peracaula, P.M. Rudd, A. McCann, Saldova. 5-AZA-dC induces epigenetic changes associated with modified glycosylation of secreted glycoproteins and increased EMT and migration in chemo-sensitive cancer cells. Clin. Epigenetics 13, 34 (2021)

Article  CAS  Google Scholar 

Y. Yang, J. Wu, F. Liu, J. He, F. Wu, J. Chen, Z. Jiang, Promotes the liver cancer stem cell phenotype by regulating MGAT5 mRNA stability by m6A RNA methylation. Stem Cells Dev 30, 1115–1125 (2021)

CAS  Google Scholar 

G. Yan, Y. Li, L. Zhan, S. Sun, J. Yuan, T. Wang, Y. Yin, Z. Dai, Y. Zhu, Z. Jiang, L. Liu, Y. Fan, F. Yang, W. Hu, Decreased mir-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5. Am. J. Cancer Res 9, 585–596 (2019)

CAS  Google Scholar 

S. Marshall, V. Bacote, R.R. Traxinger, Discovery of a metabolic pathway mediating glucose-induced desensitization of the glucose transport system. Role of hexosamine biosynthesis in the induction of insulin resistance. J. Biol. Chem 266, 4706–4712 (1991)

Article  CAS  Google Scholar 

K. Sasai, Y. Ikeda, T. Fujii, T. Tsuda, N. Taniguchi, UDP-GlcNAc concentration is an important factor in the biosynthesis of beta1,6-branched oligosaccharides: regulation based on the kinetic properties of N-acetylglucosaminyltransferase V. Glycobiology 12, 119–127 (2002)

Article  CAS  Google Scholar 

G. Kroemer, J. Pouyssegur, Tumor cell metabolism: cancer’s Achilles’ heel. Cancer Cell 13, 472–482 (2008)

Article  CAS  Google Scholar 

N.M. Akella, L. Ciraku, M.J. Reginato, Fueling the fire: emerging role of the hexosamine biosynthetic pathway in cancer. BMC Biol 17, 52 (2019)

Article  CAS  Google Scholar 

K.S. Lau, J.W. Dennis, N-Glycans in cancer progression. Glycobiology 18, 750–760 (2008)

Article  CAS  Google Scholar 

Y. Kizuka, N. Taniguchi, Enzymes for N-Glycan branching and their genetic and nongenetic regulation in cancer. Biomolecules 6, 25 (2016)

Article  Google Scholar 

Y. Zhao, T. Nakagawa, S. Itoh, K. Inamori, T. Isaji, Y. Kariya, A. Kondo, E. Miyoshi, K. Miyazaki, N. Kawasaki, N. Taniguchi, J. Gu, N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha3beta1 integrin-mediated cell migration. J. Biol. Chem 281, 32122–32130 (2006)

Article  CAS  Google Scholar 

Q. Xu, R. Akama, T. Isaji, Y. Lu, H. Hashimoto, Y. Kariya, T. Fukuda, Y. Du, J. Gu, Wnt/beta-catenin signaling down-regulates N-acetylglucosaminyltransferase III expression: the implications of two mutually exclusive pathways for regulation. J. Biol. Chem 286, 4310–4318 (2011)

Article  CAS  Google Scholar 

R.F. Osuka, T. Hirata, M. Nagae, M. Nakano, H. Shibata, R. Okamoto, Y. Kizuka, N-acetylglucosaminyltransferase-V requires a specific noncatalytic luminal domain for its activity toward glycoprotein substrates. J. Biol. Chem 298, 101666 (2022)

Article  CAS  Google Scholar 

M. Nagae, Y. Yamaguchi, N. Taniguchi, Y. Kizuka, 3D structure and function of glycosyltransferases involved in N-glycan maturation. Int. J. Mol. Sci 21, 437 (2020)

Article  CAS  Google Scholar 

M. Voss, U. Künzel, F. Higel, P.H. Kuhn, A. Colombo, A. Fukumori, M. Haug-Kröper, B. Klier, G. Grammer, A. Seidl, B. Schröder, R. Obst, H. Steiner, S.F. Lichtenthaler, C. Haass, R. Fluhrer, Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J 33, 2890–2905 (2014)

Article  CAS  Google Scholar 

M. Terao, A. Ishikawa, S. Nakahara, A. Kimura, A. Kato, K. Moriwaki, Y. Kamada, H. Murota, N. Taniguchi, I. Katayama, E. Miyoshi, Enhanced epithelial-mesenchymal transition-like phenotype in N-acetylglucosaminyltransferase V transgenic mouse skin promotes wound healing. J. Biol. Chem 286, 28303–28311 (2011)

Article  CAS  Google Scholar 

E. Miyoshi, M. Terao, Y. Kamada, Physiological roles of N-acetylglucosaminyltransferase V(GnT-V) in mice. BMB Rep 45, 554–559 (2012)

Article  CAS  Google Scholar 

A. Kimura, M. Terao, A. Kato, T. Hanafusa, H. Murota, I. Katayama, E. Miyoshi, Upregulation of N-acetylglucosaminyltransferase-V by heparin-binding EGF-like growth factor induces keratinocyte proliferation and epidermal hyperplasia. Exp. Dermatol 21, 515–519 (2012)

Article  CAS  Google Scholar 

S.S. Pinho, J. Figueiredo, J. Cabral, S. Carvalho, J. Dourado, A. Magalhães, F. Gärtner, A.M. Mendonfa, T. Isaji, J. Gu, F. Carneiro, R. Seruca, N. Taniguchi, C.A. Reis, E-cadherin and adherens-junctions stability in gastric carcinoma: functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and V. Biochim. Biophys. Acta 1830, 2690–2700 (2013)

Article  CAS  Google Scholar 

M. Yoshimura, Y. Ihara, Y. Matsuzawa, N. Taniguchi, Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis. J. Biol. Chem 271, 13811–13815 (1996)

Article  CAS  Google Scholar 

A. Lagana, J.G. Goetz, P. Cheung, A. Raz, J.W. Dennis, I.R. Nabi, Galectin binding to Mgat5-modified N-glycans regulates fibronectin matrix remodeling in tumor cells. Mol. Cell. Biol 26, 3181–3193 (2006)

Article  CAS  Google Scholar 

M.D. Langer, H. Guo, N. Shashikanth, J.M. Pierce, D.E. Leckband, N-glycosylation alters cadherin-mediated intercellular binding kinetics. J. Cell. Sci 125, 2478–2485 (2012)

CAS  Google Scholar 

P. Cheung, J. Pawling, E.A. Partridge, B. Sukhu, M. Grynpas, J.W. Dennis, Metabolic homeostasis and tissue renewal are dependent on beta1,6GlcNAc-branched N-glycans. Glycobiology 17, 828–837 (2007)

Article  CAS  Google Scholar 

M.C. Ryczko, J. Pawling, R. Chen, A.M. Abdel Rahman, K. Yau, J.K. Copeland, C. Zhang, A. Surendra, D.S. Guttman, D. Figeys, J.W. Dennis, Metabolic reprogramming by hexosamine biosynthetic and golgi N-Glycan branching pathways. Sci. Rep 6, 23043 (2016)

Article  CAS  Google Scholar 

A. Johswich, C. Longuet, J. Pawling, A. Abdel Rahman, M. Ryczko, D.J. Drucker, J.W. Dennis, N-glycan remodeling on glucagon receptor is an effector of nutrient sensing by the hexosamine biosynthesis pathway. J. Biol. Chem 289, 15927–15941 (2014)

Article  CAS  Google Scholar 

A.M. Abdel Rahman, M. Ryczko, M. Nakano, J. Pawling, T. Rodrigues, A. Johswich, N. Taniguchi, J.W. Dennis, Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism. Glycobiology 25, 225–240 (2015)

Article  CAS  Google Scholar 

K.S. Lau, E.A. Partridge, A. Grigorian, C.I. Silvescu, V.N. Reinhold, M. Demetriou, J.W. Dennis, Complex N-glycan number and degree of branching cooperate to regulate cell proliferation and differentiation. Cell 129, 123–134 (2007)

Article  CAS  Google Scholar 

J.W. Dennis, J. Pawling, P. Cheung, E. Partridge, M. Demetriou, UDP-N-acetylglucosamine:alpha-6-D-mannoside beta1,6 N-acetylglucosaminyltransferase V (Mgat5) deficient mice. Biochim. Biophys. Acta 1573, 414–422 (2002)

Article  CAS  Google Scholar 

M. Demetriou, M. Granovsky, S. Quaggin, J.W. Dennis, Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature 409, 733–739 (2001)

Article  CAS  Google Scholar 

J. Kuball, B. Hauptrock, V. Malina, E. Antunes, R.H. Voss, M. Wolfl, R. Strong, M. Theobald, P.D. Greenberg, Increasing functional avidity of TCR-redirected T cells by removing defined N-glycosylation sites in the TCR constant domain. J. Exp. Med 206, 463–475 (2009)

Article  CAS  Google Scholar 

A.M. Dias, A. Correia, M.S. Pereira, C.R. Almeida, I. Alves, V. Pinto, T.A. Catarino, N. Mendes, M. Leander, M.T. Oliva-Teles, L. Maia, C. Delerue-Matos, N. Taniguchi, M. Lima, I. Pedroto, R. Marcos-Pinto, P. Lago, C.A. Reis, M. Vilanova, S.S. Pinho, Metabolic control of T cell immune response through glycans in inflammatory bowel disease. Proc. Natl. Acad. Sci. U.S.A. 115, E4651-E4660 (2018)

Y. Kamada, K. Mori, H. Matsumoto, S. Kiso, Y. Yoshida, S. Shinzaki, N. Hiramatsu, M. Ishii, K. Moriwaki, N. Kawada, T. Takehara, E. Miyoshi, N-Acetylglucosaminyltransferase V regulates TGF-β response in hepatic stellate cells and the progression of steatohepatitis. Glycobiology 22, 778–787 (2012)

Article  CAS  Google Scholar 

A.M. Dias, J. Dourado, P. Lago, J. Cabral, R. Marcos-Pinto, P. Salgueiro, C.R. Almeida, S. Carvalho, S. Fonseca, M. Lima, M. Vilanova, M. Dinis-Ribeiro, C.A. Reis, S.S. Pinho, Dysregulation of T cell receptor N-glycosylation: a molecular mechanism involved in ulcerative colitis. Hum. Mol. Genet 23, 2416–2427 (2014)

Article  CAS  Google Scholar 

T. Saito, E. Miyoshi, K. Sasai, N. Nakano, H. Eguchi, K. Honke, N. Taniguchi, A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity. J. Biol. Chem 277, 17002–17008 (2002)