Structural basis for intermodular communication in assembly-line polyketide biosynthesis

Newman, D. J. & Cragg, G. M. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. J. Nat. Prod. 83, 770–803 (2020).

Article  CAS  PubMed  Google Scholar 

Robertsen, H. L. & Musiol-Kroll, E. M. Actinomycete-derived polyketides as a source of antibiotics and lead structures for the development of new antimicrobial drugs. Antibiotics (Basel) 8, 157 (2019).

Kirst, H. A. The spinosyn family of insecticides: realizing the potential of natural products research. J. Antibiot. (Tokyo) 63, 101–111 (2010).

Article  CAS  PubMed  Google Scholar 

Toopaang, W. et al. Microbial polyketides and their roles in insect virulence: from genomics to biological functions. Nat. Prod. Rep. 39, 2008–2029 (2022).

Article  CAS  PubMed  Google Scholar 

Nivina, A., Yuet, K. P., Hsu, J. & Khosla, C. Evolution and diversity of assembly-line polyketide synthases. Chem. Rev. 119, 12524–12547 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Klaus, M. & Grininger, M. Engineering strategies for rational polyketide synthase design. Nat. Prod. Rep. 35, 1070–1081 (2018).

Article  CAS  PubMed  Google Scholar 

Soohoo, A. M., Cogan, D. P., Brodsky, K. L. & Khosla, C. Structure and mechanisms of assembly-line polyketide synthases. Annu. Rev. Biochem. 3, 471–498 (2024).

Article  Google Scholar 

Dutta, S. et al. Structure of a modular polyketide synthase. Nature 510, 512–517 (2014).

Article  PubMed  PubMed Central  Google Scholar 

Whicher, J. R. et al. Structural rearrangements of a polyketide synthase module during its catalytic cycle. Nature 510, 560–564 (2014).

Article  PubMed  PubMed Central  Google Scholar 

Herbst, D. A. et al. The structural organization of substrate loading in iterative polyketide synthases. Nat. Chem. Biol. 14, 474–479 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang, J. et al. Structural basis for the biosynthesis of lovastatin. Nat. Commun. 12, 867 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cogan, D. P. et al. Mapping the catalytic conformations of an assembly-line polyketide synthase module. Science 374, 729–734 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bagde, S. R., Mathews, I. I., Fromme, J. C. & Kim, C.-Y. Modular polyketide synthase contains two reaction chambers that operate asynchronously. Science 374, 723–729 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Grininger, M. Enzymology of assembly line synthesis by modular polyketide synthases. Nat. Chem. Biol. 19, 401–415 (2023).

Article  CAS  PubMed  Google Scholar 

Kao, C. M., Pieper, R., Cane, D. E. & Khosla, C. Evidence for two catalytically independent clusters of active sites in a functional modular polyketide synthase. Biochemistry 35, 12363–12368 (1996).

Article  CAS  PubMed  Google Scholar 

Witkowski, A. et al. Dibromopropanone cross-linking of the phosphopantetheine and active-site cysteine thiols of the animal fatty acid synthase can occur both inter- and intrasubunit. Reevaluation of the side-by-side, antiparallel subunit model. J. Biol. Chem. 274, 11557–11563 (1999).

Article  CAS  PubMed  Google Scholar 

Stoops, J. K. & Wakil, S. J. Yeast fatty acid synthetase: structure–function relationship and nature of the β-ketoacyl synthetase site. Proc. Natl Acad. Sci. USA 77, 4544–4548 (1980).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Miyazawa, T., Hirsch, M., Zhang, Z. & Keatinge-Clay, A. T. An in vitro platform for engineering and harnessing modular polyketide synthases. Nat. Commun. 11, 80 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gokhale, R. S., Tsuji, S. Y., Cane, D. E. & Khosla, C. Dissecting and exploiting intermodular communication in polyketide synthases. Science 284, 482–485 (1999).

Article  CAS  PubMed  Google Scholar 

Broadhurst, R. W., Nietlispach, D., Wheatcroft, M. P., Leadlay, P. F. & Weissman, K. J. The structure of docking domains in modular polyketide synthases. Chem. Biol. 10, 723–731 (2003).

Article  CAS  PubMed  Google Scholar 

Tsuji, S. Y., Cane, D. E. & Khosla, C. Selective protein−protein interactions direct channeling of intermediates between polyketide synthase modules. Biochemistry 40, 2326–2331 (2001).

Article  CAS  PubMed  Google Scholar 

Henderson, R. et al. Outcome of the first electron microscopy validation task force meeting. Structure 20, 205–214 (2012).

Article  CAS  PubMed  Google Scholar 

Kapur, S., Chen, A. Y., Cane, D. E. & Khosla, C. Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase. Proc. Natl Acad. Sci. USA 107, 22066–22071 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kapur, S. et al. Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation. Proc. Natl Acad. Sci. USA 109, 4110–4115 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Charkoudian, L. K. et al. Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase. Protein Sci. 20, 1244–1255 (2011).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Squire, C. M., Goss, R. J. M., Hong, H., Leadlay, P. F. & Staunton, J. Catalytically active tetramodular 6-deoxyerythonolide B synthase fusion proteins. ChemBioChem 4, 1225–1228 (2003).

Article  CAS  PubMed  Google Scholar 

Cogan, D. P. et al. Antibody probes of module 1 of the 6-deoxyerythronolide B synthase reveal an extended conformation during ketoreduction. J. Am. Chem. Soc. 142, 14933–14939 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhao, Y., Schmid, M. F., Frydman, J. & Chiu, W. CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin. Nat. Commun. 12, 4754 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tsai, S. C. et al. Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel. Proc. Natl Acad. Sci. USA 98, 14808–14813 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Klaus, M. et al. Protein–protein interactions, not substrate recognition, dominate the turnover of chimeric assembly line polyketide. J. Biol. Chem. 291, 16404–16415 (2016).

Whicher, J. R. et al. Cyanobacterial polyketide synthase docking domains: a tool for engineering natural product biosynthesis. Chem. Biol. 20, 1340–1351 (2013).

Article  CAS  PubMed  Google Scholar 

Zhang, L. et al. Characterization of giant modular PKSs provides insight into

留言 (0)

沒有登入
gif