HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics

Tzingounis, A. V. & Wadiche, J. I. Glutamate transporters: confining runaway excitation by shaping synaptic transmission. Nat. Rev. Neurosci. 8, 935–947 (2007).

Article  CAS  PubMed  Google Scholar 

Danbolt, N. C. Glutamate uptake. Prog. Neurobiol. 65, 1–105 (2001).

Article  CAS  PubMed  Google Scholar 

Yernool, D., Boudker, O., Jin, Y. & Gouaux, E. Structure of a glutamate transporter homologue from Pyrococcus horikoshii. Nature 431, 811–818 (2004).

Article  CAS  PubMed  Google Scholar 

Wang, X. & Boudker, O. Large domain movements through the lipid bilayer mediate substrate release and inhibition of glutamate transporters. eLife 9, e58417 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Verdon, G. & Boudker, O. Crystal structure of an asymmetric trimer of a bacterial glutamate transporter homolog. Nat. Struct. Mol. Biol. 19, 355–357 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Reyes, N., Ginter, C. & Boudker, O. Transport mechanism of a bacterial homologue of glutamate transporters. Nature 462, 880–885 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Reyes, N., Oh, S. & Boudker, O. Binding thermodynamics of a glutamate transporter homolog. Nat. Struct. Mol. Biol. 20, 634–640 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Verdon, G., Oh, S., Serio, R. N. & Boudker, O. Coupled ion binding and structural transitions along the transport cycle of glutamate transporters. eLife 3, e02283 (2014).

Article  PubMed  PubMed Central  Google Scholar 

Arkhipova, V. et al. Binding and transport of d-aspartate by the glutamate transporter homolog GltTk. eLife https://doi.org/10.7554/eLife.45286 (2019).

Garaeva, A. A., Guskov, A., Slotboom, D. J. & Paulino, C. A one-gate elevator mechanism for the human neutral amino acid transporter ASCT2. Nat. Commun. 10, 3427 (2019).

Article  PubMed  PubMed Central  Google Scholar 

Arkhipova, V., Guskov, A. & Slotboom, D. J. Structural ensemble of a glutamate transporter homologue in lipid nanodisc environment. Nat. Commun. 11, 998 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Guskov, A., Jensen, S., Faustino, I., Marrink, S. J. & Slotboom, D. J. Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue Glt. Nat. Commun. 7, 13420 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Alleva, C. et al. Na+-dependent gate dynamics and electrostatic attraction ensure substrate coupling in glutamate transporters. Sci. Adv. 6, eaba9854 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen, I. et al. Glutamate transporters have a chloride channel with two hydrophobic gates. Nature 591, 327–331 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Groeneveld, M. & Slotboom, D. J. Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph). Biochemistry 49, 3511–3513 (2010).

Article  CAS  PubMed  Google Scholar 

Zerangue, N. & Kavanaugh, M. P. Flux coupling in a neuronal glutamate transporter. Nature 383, 634–637 (1996).

Article  CAS  PubMed  Google Scholar 

Ando, T. et al. A high-speed atomic force microscope for studying biological macromolecules. Proc. Natl Acad. Sci. USA 98, 12468–12472 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ando, T., Uchihashi, T. & Scheuring, S. Filming biomolecular processes by high-speed atomic force microscopy. Chem. Rev. 114, 3120–3188 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Akyuz, N. et al. Transport domain unlocking sets the uptake rate of an aspartate transporter. Nature 518, 68–73 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Akyuz, N., Altman, R. B., Blanchard, S. C. & Boudker, O. Transport dynamics in a glutamate transporter homologue. Nature 502, 114–118 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Erkens, G. B., Hänelt, I., Goudsmits, J. M., Slotboom, D. J. & van Oijen, A. M. Unsynchronised subunit motion in single trimeric sodium-coupled aspartate transporters. Nature 502, 119–123 (2013).

Article  CAS  PubMed  Google Scholar 

Huysmans, G. H. M., Ciftci, D., Wang, X., Blanchard, S. C. & Boudker, O. The high-energy transition state of the glutamate transporter homologue GltPh. EMBO J. 40, e105415 (2021).

Article  CAS  PubMed  Google Scholar 

Ciftci, D. et al. Single-molecule transport kinetics of a glutamate transporter homolog shows static disorder. Sci. Adv. 6, eaaz1949 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Silberberg, S. D., Lagrutta, A., Adelman, J. P. & Magleby, K. L. Wanderlust kinetics and variable Ca(2+)-sensitivity of dSlo [correction of Drosophila], a large conductance CA(2+)-activated K+ channel, expressed in oocytes. Biophys. J. 71, 2640–2651 (1996).

Article  CAS  PubMed  Google Scholar 

Poon, K., Nowak, L. M. & Oswald, R. E. Characterizing single-channel behavior of GluA3 receptors. Biophys. J. 99, 1437–1446 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hirschberg, B., Maylie, J., Adelman, J. P. & Marrion, N. V. Gating of recombinant small-conductance Ca-activated K+ channels by calcium. J. Gen. Physiol. 111, 565–581 (1998).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Milone, M. et al. Mode switching kinetics produced by a naturally occurring mutation in the cytoplasmic loop of the human acetylcholine receptor epsilon subunit. Neuron 20, 575–588 (1998).

Article  CAS  PubMed  Google Scholar 

Rothberg, B. S. & Magleby, K. L. Kinetic structure of large-conductance Ca2+-activated K+ channels suggests that the gating includes transitions through intermediate or secondary states. A mechanism for flickers. J. Gen. Physiol. 111, 751–780 (1998).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kosmidis, E. et al. Regulation of the mammalian-brain V-ATPase through ultraslow mode-switching. Nature 611, 827–834 (2022).

Article  CAS  PubMed  Google Scholar 

Ruan, Y. et al. Direct visualization of glutamate transporter elevator mechanism by high-speed AFM. Proc. Natl Acad. Sci. USA 114, 1584–1588 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Matin, T. R., Heath, G. R., Huysmans, G. H. M., Boudker, O. & Scheuring, S. Millisecond dynamics of an unlabeled amino acid transporter. Nat. Commun. 11, 5016 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Heath, G. R. & Scheuring, S. Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters. Curr. Opin. Struct. Biol. 57, 93–102 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jiang, Y. et al. Membrane-mediated protein interactions drive membrane protein organization. Nat. Commun. 13, 7373 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Phillips, R., Ursell, T., Wiggins, P. & Sens, P. Emerging roles for lipids in shaping membrane-protein function. Nature 459, 379–385 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gao, J., Hou, R., Li, L. & Hu, J. Membrane-mediated interactions between protein inclusions. Front. Mol. Biosci. 8, 811711 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Eddy, S. R. Hidden Markov models. Curr. Opin. Struct. Biol. 6, 361–365 (1996).

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