Development of a fluorescence and quencher-based FRET assay for detection of endogenous peptide:N-glycanase/NGLY1 activity
Keywords
peptide:N-glycanase
endo-β-N-acetylglucosaminidase
FRET
enzyme assay
aging
Abbreviations5FAM-GCP
5-carboxyfluorescein-labeled glycosylated hepta-cyclopeptide
AMCA
aminomethylcoumarin acetate
CNS
central nervous system
ENGase
endo-β-N-acetylglucosaminidase
ESI-MS
electrospray ionization-mass spectrometry
GCP
glycosylated hepta-cyclopeptide
GlcNAz
N-azidoacetylglucosamine
HRP
horseradish peroxidase
NMP
N-methyl-2-pyrrolidone
PA-labeled
pyridylamino-labeled
PBMC
peripheral blood mononuclear cells
© 2024 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.
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