salt-inducible kinase
isoform selectivity
crystallization chaperones
AMPK related kinase
drug design
crystal structure
protein purification
protein expression
protein kinase
protein crystallization
Abbreviations2TYAG2TY+ ampicillin + glucose
ABLAbelson murine leukemia viral oncogene
AMPKAMP-activated protein kinase
BCRbreakpoint cluster region
BIS-TRIS propane1,3-bis(tris(hydroxymethyl)methylamino)propane
BRSK2BR serine/threonine kinase 2
CDRcomplementarity determining region
CREBcAMP-response element binding protein
CRTCsCREB-regulated transcriptional coactivators
Fabfragment antigen-binding
GSTglutathione S-transferase
HEKhuman embryonic kidney
IEXion exchange chromatography
IMACimmobilized metal ion affinity chromatography
IPTGisopropyl β-d-1-thiogalactopyranoside
MARKMAP/microtubule affinity-regulating kinase
MEF2Myosite Enhancer Factor 2
MELKmaternal embryonic leucine zipper kinase
MMSmicroseed matrix screening
MPD2-methyl-2,4-pentanediol
MSTmammalian STE20-like protein kinase
NCSnon-crystallographic symmetry
NUAKSNF1/AMP kinase-related kinase
PAGEpolyacrylamide gel electrophoresis
r.m.s.d.root-mean-square deviation
ScFvsingle-chain variable fragment
SERsurface-entrope reducing
SPRsurface plasmon resonance
SrcProto-oncogene tyrosine-protein kinase
TCEPtris(2-ccarboxyethyl)phosphine
TEVtobacco etch virus nuclear-inclusion-a endopeptidase
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