Bannikov A.V., Lavrov A.V. 2017. CRISPR/Cas9, the king of genome editing tools. Mol. Biol. (Moscow). 51, 514–525. https://doi.org/10.1134/S0026893317040033

Article  CAS  Google Scholar 

Wiedenheft B., Sternberg S.H., Doudna J.A. 2012. RNA-guided genetic silencing systems in bacteria and archaea. Nature. 482, 331–338.

Article  ADS  CAS  Google Scholar 

Ran F.A., Hsu P.D., Wright J., Agarwala V., Scott D.A., Zhang F. 2013. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281–2308.

Article  CAS  Google Scholar 

Kostyushev D., Brezgin S., Kostyusheva A., Zarifyan D., Goptar I., Chulanov V. 2019. Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus. Cell. Mol. Life Sci. 76, 1779–1794.

Article  CAS  Google Scholar 

Brezgin S., Kostyusheva A., Kostyushev D., Chulanov V. 2019. Dead Cas systems: Types, principles, and applications. Int. J. Mol. Sci. 20 (23), 6041.

Article  CAS  Google Scholar 

Kostyusheva A., Brezgin S., Babin Y., Vasilyeva I., Glebe D., Kostyushev D., Chulanov V. 2022. CRIS-PR−Cas systems for diagnosing infectious diseases. Methods. 203, 431–446.

Article  CAS  Google Scholar 

Chen S., Lee B., Lee A.Y., Modzelewski A.J., He L. 2016. Highly efficient mouse genome editing by CR-ISPR ribonucleoprotein electroporation of zygotes. J. Biol. Chem. 291 (28), 14457–14467.

Article  CAS  Google Scholar 

Bharathkumar N., Sunil A., Meera P., Aksah S., Kannan M., Saravanan K.M., Anand T. 2022. CRI-SPR/Cas‑based modifications for therapeutic applications: A review. Mol. Biotechnol. 64, 355–372.

Article  CAS  Google Scholar 

Brezgin S., Kostyusheva A., Ponomareva N., Volia V., Goptar I., Nikiforova A., Shilovskiy I., Smirnov V., Kostyushev D., Chulanov V. 2020. Clearing of foreign episomal DNA from human cells by CRISPRa-mediated activation of cytidine deaminases. Int. J. Mol. Sci. 21, 1–17.

Article  Google Scholar 

Kostyushev D., Brezgin S., Kostyusheva A., Ponomareva N., Bayurova E., Zakirova N., Kondra-shova A., Goptar I., Nikiforova A., Sudina A., Babin Y., Gordeychuk I., Lukashev A., Zamyatnin A., Ivanov A., Chulanov V. 2023. Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus. Mol. Ther. Nucl. Acids. 32, 478–493.

Article  CAS  Google Scholar 

Kostyushev D., Kostyusheva A., Brezgin S., Ponomareva N., Zakirova N.F., Egorshina A., Yanvarev D.V., Bayurova E., Sudina A., Goptar I., Nikiforova A., Dunaeva E., Lisitsa T., Abramov I., Frolova A., Lukashev A., Gordeychuk I., Zamyatnin A.A., Ivanov A., Chulanov V. 2023. Depleting hepatitis B virus relaxed circular DNA is necessary for resolution of infection by CRISPR-Cas9. Mol. Ther. Nucl. Acids, 31, 482–493.

Article  CAS  Google Scholar 

Kostyusheva A.P., Kostyushev D.S., Brezgin S.A., Zarifyan D.N., Volchkova E.V., Chulanov V.P. 2019. Small molecular inhibitors of DNA double strand break repair pathways increase the ANTI-HBV activity of CRISPR/Cas9. Mol. Biol. (Moscow). 53, 274–285. https://doi.org/10.1134/S0026893319010072

Article  CAS  Google Scholar 

Kostyusheva A.P., Brezgin S.A., Ponomareva N.I., Goptar I.A., Nikiforova A.V., Gegechkori V.I., Poluektova V.B., Turkadze K.A., Sudina A.E., Chulanov V.P., Kostyushev D.S. 2022. Antiviral activity of CRISPR/Cas9 ribonucleoprotein complexes on a hepatitis B virus model in vivo. Mol. Biol. (Moscow). 56, 816‒822. https://doi.org/10.1134/S0026893322060097

Article  CAS  Google Scholar 

Sharma G., Sharma A.R., Bhattacharya M., Lee S., Chakraborty C. 2021. CRISPR-Cas9: A preclinical and clinical perspective for the treatment of human diseases. Mol. Ther. 29 (2), 571–586.

Article  CAS  Google Scholar 

Rafii S., Tashkandi E., Bukhari N., Al-shamsi H.O. 2022. Current status of CRISPR/Cas9 application in clinical cancer research: Opportunities and challenges. Cancers (Basel). 14 (4), 1–14.

Article  Google Scholar 

Kostyushev D., Kostyusheva A., Brezgin S., Smirnov V., Volchkova E., Lukashev A., Chulanov V. 2020. Gene editing by extracellular vesicles. Int. J. Mol. Sci. 21, 1–34.

Article  Google Scholar 

Li A., Lee C.M., Hurley A.E., Jarrett K.E., De Giorgi M., Lu W., Balderrama K.S., Doerfler A.M., Deshmukh H., Ray A., Bao G., Lagor W.R. 2019. A self-deleting AAV-CRISPR system for in vivo genome editing. Mol. Ther. Methods Clin. Dev. 12, 111–122.

Article  CAS  Google Scholar 

Scott T., Moyo B., Nicholson S., Maepa M.B., Watashi K., Ely A., Weinberg M.S., Arbuthnot P. 2017. SsAAVs containing cassettes encoding SaCas9 and guides targeting hepatitis B virus inactivate replication of the virus in cultured cells. Sci. Rep. 7 (1), 7401.

Article  ADS  Google Scholar 

Bulcha J.T., Wang Y., Ma H., Tai P.W.L., Gao G. 2021. Viral vector platforms within the gene therapy landscape. Signal Transduct. Target. Ther. 6 (1), 53.

Article  CAS  Google Scholar 

Jiang C., Mei M., Li B., Zhu X., Zu W., Tian Y., Wang Q., Guo Y., Dong Y., Tan X. 2017. A non-viral CRISPR/Cas9 delivery system for therapeutically targeting HBV DNA and pcsk9 in vivo. Cell. Res. 27 (3), 440–443.

Article  CAS  Google Scholar 

Vader P., Mol E.A., Pasterkamp G., Schiffelers R.M. 2016. Extracellular vesicles for drug delivery. Adv. Drug Delivery Rev. 106, 148–156.

Article  CAS  Google Scholar 

Doyle L.M., Wang M.Z. 2019. Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis. Cells. 8 (7), 727.

Article  CAS  Google Scholar 

Phillips W., Willms E., Hill A.F. 2021. Understanding extracellular vesicle and nanoparticle heterogeneity: Novel methods and considerations. Proteomics. 21 (13–14), e2000118.

Article  Google Scholar 

Alvarez-erviti L., Seow Y., Yin H., Betts C., Lakhal S., Wood M.J.A. 2011. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat. Biotechnol. 29 (4), 341–345.

Article  CAS  Google Scholar 

Yang B., Chen Y., Shi J. 2019. Exosome biochemistry and advanced nanotechnology for next-generation theranostic platforms. Adv. Mater. 31 (2), e1802896.

Article  Google Scholar 

Kim M.S., Haney M.J., Zhao Y., Mahajan V., Deygen I., Klyachko N.L., Inskoe E., Piroyan A., Sokolsky M., Okolie O., Hingtgen S.D., Kabanov A.V., Batrakova E.V. 2016. Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells. Nanomedicine. 12 (3), 655–664.

Article  CAS  Google Scholar 

Yuan D., Zhao Y., Banks W.A., Bullock K.M., Haney M., Batrakova E., Kabanov A.V. 2017. Macrophage exosomes as natural nanocarriers for protein delivery to inflamed brain. Biomaterials. 142, 1–12.

Article  CAS  Google Scholar 

Kim H., Yun N., Mun D., Kang J.Y., Lee S.H., Park H., Park H., Joung B. 2018. Cardiac-specific delivery by cardiac tissue-targeting peptide-expressing exosomes. Biochem. Biophys. Res. Commun. 499 (4), 803–808.

Article  CAS  Google Scholar 

Brezgin S., Parodi A., Kostyusheva A., Ponomar-va N., Lukashev A., Sokolova D., Pokrovsky V.S., Slatinskaya O., Maksimov G., Zamyatnin A.A. Jr., Chulanov V., Kostyushev D. 2023. Technological aspects of manufacturing and analytical control of biological nanoparticles. Biotechnol. Adv. 64, 108122.

Article  CAS  Google Scholar 

Chen R., Huang H., Liu H., Xi J., Ning J., Zeng W., Shen C., Zhang T., Yu G., Xu Q., Chen X., Wang J., Lu F. 2019. Friend or foe? Evidence indicates endogenous exosomes can deliver functional gRNA and Cas9 protein. Small. 15 (38), e1902686.

Article  Google Scholar 

Dai J., Su Y., Zhong S., Cong L., Liu B., Yang J., Tao Y., He Z., Chen C., Jiang Y. 2020. Exosomes: Key players in cancer and potential therapeutic strategy. Signal. Transduct. Target. Ther. 5 (1), 145.

Article  CAS  Google Scholar 

Kornilov R., Puhka M., Mannerström B., Hiidenmaa H., Peltoniemi H., Siljander P., Seppänen-Kaijansinkko R., Kaur S. 2018. Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum. J. Extracell. Vesicles. 7 (1), 1422674.

Article  Google Scholar 

Hessvik N.P., Llorente A. 2018. Current knowledge on exosome biogenesis and release. Cell. Mol. Life Sci. 75 (2), 193–208.

Article  CAS  Google Scholar 

Ostrowski M., Carmo N.B., Krumeich S., Fanget I., Raposo G., Savina A., Moita C.F., Schauer K., Hume A.N., Freitas R.P., Goud B., Benaroch P., Hacohen N., Fukuda M., Desnos C., Seabra M.C., Darchen F., Amigorena S., Moita L.F., Thery C. 2009. Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat. Cell Biol. 12 (1), 19–30.

Article  Google Scholar 

Gurung S., Perocheau D., Touramanidou L., Baruteau J. 2021. The exosome journey: From biogenesis to uptake and intracellular signalling. Cell Commun. Signal. 19 (1), 47.

Article  CAS  Google Scholar 

Manders E.M., Verbeek F., Aten J. 1992. Measurement of co-localization of objects in dual colour confocal images. J. Microsc. 169, 375–382.

Article  Google Scholar 

Luo T., Mitra S., McBride J.W. 2018. Ehrlichia chaffeensis TRP75 interacts with host cell targets. mSphere. 3 (2), e00147-18.

Article  CAS  Google Scholar 

Brezgin S., Kostyusheva A., Ponomareva N., Bayurova E., Kondrashova A., Frolova A., Slatinskaya O., Fatkhutdinova L., Maksimov G., Zyuzin M., Gordeychuk I., Lukashev A., Makarov S., Ivanov A., Zamyatnin A.A., Jr., Chulanov V., Parodi A., Kostyushev D. 2023. Hydroxychloroquine enhances cytotoxic properties of extracellular vesicles and extracellular vesicle—mimetic nanovesicles loaded with chemotherapeutics. Pharmaceutics. 15 (2), 534.

Article  CAS  Google Scholar 

Théry C., Witwer K.W., Aikawa E., Alcaraz M. J., Anderson J.D., Andriantsitohaina R., Antoniou A., Arab T., Archer F., Atkin-Smith G.K. 2018. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for extracellular vesicles and update of the MISEV2014 guidelines. J. Extracell. Vesicles. 7 (1), 1535750.

Article  Google Scholar 

Witwer K.W., Goberdhan D.C., O’Driscoll L., Théry C., Welsh J.A., Blenkiron C., Buzás E.I., Di Vizio D., Erdbrügger U., Falcón-Pérez J.M., Fu Q.L, Hill A.F., Lenassi M., Lötvall J., Nieuwland R., Ochiya T., Rome S., Sahoo S., Zheng L. 2021. Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles. J. Extracell. Vesicles. 10 (14), e12182.

Article  CAS