Andre CM, Hausman J-F, Guerriero G. Cannabis sativa: the plant of the thousand and one molecules. Front Plant Sci. 2016;7 .https://doi.org/10.3389/fpls.2016.00019.

Zou S, Kumar U. Cannabinoid receptors and the Endocannabinoid system: signaling and function in the central nervous system. Int J Mol Sci. 2018;19:833. https://doi.org/10.3390/ijms19030833.

Article  CAS  PubMed Central  Google Scholar 

National Academies of Sciences E, Medicine, Health, Medicine D, Board on Population H, Public Health P et al. The National Academies Collection: Reports funded by National Institutes of Health. The health effects of cannabis and cannabinoids: the current state of evidence and recommendations for research. Washington (DC): National Academies Press (US).

Burstein SH. The cannabinoid acids: nonpsychoactive derivatives with therapeutic potential. Pharmacol Ther. 1999;82:87–96. https://doi.org/10.1016/S0163-7258(98)00069-2.

Article  CAS  Google Scholar 

McPartland JM, MacDonald C, Young M, Grant PS, Furkert DP, Glass M. Affinity and efficacy studies of tetrahydrocannabinolic acid A at cannabinoid receptor types one and two. Cannabis Cannabinoid Res. 2017;2:87–95. https://doi.org/10.1089/can.2016.0032.

Article  CAS  PubMed Central  Google Scholar 

Lewis MM, Yang Y, Wasilewski E, Clarke HA, Kotra LP. Chemical profiling of medical cannabis extracts. ACS Omega. 2017;2:6091–103. https://doi.org/10.1021/acsomega.7b00996.

Article  CAS  PubMed Central  Google Scholar 

Martinenghi LD, Jønsson R, Lund T, Jenssen H. Isolation, purification, and antimicrobial characterization of cannabidiolic acid and cannabidiol from Cannabis sativa L. Biomolecules. 2020;10. https://doi.org/10.3390/biom10060900.

Popp JR, Petrakis EA, Angelis A, Halabalaki M, Bonn GK, Stuppner H, et al. Rapid isolation of acidic cannabinoids from Cannabis sativa L. using pH-zone-refining centrifugal partition chromatography. J Chromatogr A. 2019;1599:196–202. https://doi.org/10.1016/j.chroma.2019.04.048.

Article  CAS  Google Scholar 

Harvey AL, Edrada-Ebel R, Quinn RJ. The re-emergence of natural products for drug discovery in the genomics era. Nat Rev Drug Discov. 2015;14:111–29. https://doi.org/10.1038/nrd4510.

Article  CAS  Google Scholar 

Dadiotis E, Mitsis V, Melliou E, Magiatis P. Direct Quantitation of Phytocannabinoids by One-Dimensional 1H qNMR and Two-Dimensional 1H-1H COSY qNMR in Complex Natural Mixtures. Molecules. 2022;27:2965.

Article  CAS  PubMed Central  Google Scholar 

Aizikovich A. Anticancer Effect of New Cannabinoids Derived from Tetrahydrocannabinolic Acid on PANC-1 and AsPC-1 Human Pancreas Tumor Cells. J Pancreat Cancer. 2020;6:40–4. https://doi.org/10.1089/pancan.2020.0003.

Article  PubMed Central  Google Scholar 

Suzuki M, Takeda S, Okazaki H, Watanabe K, Takiguchi M, Aramaki H. Cannabidiolic Acid-Mediated Interference with AP-1 Transcriptional Activity in MDA-MB-231 Breast Cancer Cells. Nat Prod Commun. 2017;12:759–61.

Google Scholar 

Nahler G. Cannabidiol and other phytocannabinoids as cancer therapeutics. Pharmaceut Med. 2022;36:99–129. https://doi.org/10.1007/s40290-022-00420-4.

Article  CAS  PubMed Central  Google Scholar 

Sainz-Cort A, Müller-Sánchez C, Espel E. Anti-proliferative and cytotoxic effect of cannabidiol on human cancer cell lines in presence of serum. BMC Res Notes. 2020;13:389. https://doi.org/10.1186/s13104-020-05229-5.

Article  CAS  PubMed Central  Google Scholar 

Durante C, Anceschi L, Brighenti V, Caroli C, Afezolli C, Marchetti A, et al. Application of experimental design in HPLC method optimisation for the simultaneous determination of multiple bioactive cannabinoids. J Pharm Biomed Anal. 2022;221:115037. https://doi.org/10.1016/j.jpba.2022.115037.

Article  CAS  Google Scholar 

Janatová A, Doskočil I, Božik M, Fraňková A, Tlustoš P, Klouček P. The chemical composition of ethanolic extracts from six genotypes of medical cannabis (Cannabis sativa L.) and their selective cytotoxic activity. Chem Interact. 2022;353:109800.

Article  Google Scholar 

Shor DB-A, Hochman I, Gluck N, Shibolet O, Scapa E. The Cytotoxic Effect of Isolated Cannabinoid Extracts on Polypoid Colorectal Tissue. Int J Mol Sci. 2022;23:11366.

Article  Google Scholar 

Tomko AM, Whynot EG, Ellis LD, Dupré DJ. Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis. Cancers. 2020;12:1985.

Article  CAS  PubMed Central  Google Scholar 

Helcman M, Šmejkal K. Biological activity of Cannabis compounds: A modern approach to the therapy of multiple diseases. Phytochem Rev. 2022;21:429–70.

Article  CAS  Google Scholar 

Mingaleeva RN, Solovieva VV, Blatt NL, Rizvanov AA. Application of cell and tissue cultures for potential anti-cancer/oncology drugs screening in vitro. Cell. Transpl. Tissue Eng. 2013;8:20–8.

Google Scholar 

Open Targets Platform. https://platform.opentargets.org/ (accessed 2023).

Open Targets: A Platform for Therapeutic Target Identification and Validation.

Bank PD. Protein Data Bank. Nat New Biol. 1971;233:223.

Article  Google Scholar 

RCSB PDB: Homepage. https://www.rcsb.org/ (accessed 2023).

Trott O, Olson AJ. AutoDock Vina: Improving the Speed and Accuracy of Docking with a New Scoring Function, Efficient Optimization, and Multithreading. J Comput Chem. 2010;31:455–61.

Article  CAS  PubMed Central  Google Scholar 

Eswar N, Eramian D, Webb B, Shen M-Y, Sali A. Protein Structure Modeling with MODELLER. In Structural Proteomics; Springer, 2008; pp 145–59.

DE Shaw Research. Desmond Molecular Dynamics System. 2016.

DeLano WL. The PyMOL Molecular Graphics System. 2002.

Jiménez J, Škalič M, Martínez-Rosell G, De Fabritiis G. K DEEP: Protein–Ligand Absolute Binding Affinity Prediction via 3D-Convolutional Neural Networks. J Chem Inf Model. 2018;58:287–96. https://doi.org/10.1021/acs.jcim.7b00650.

Article  CAS  Google Scholar