Advances in Pharmacology and Pharmacy Vol. 12(2), pp. 95 - 103
DOI: 10.13189/app.2024.120202
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Sachin Shivling Bhusari *, Akshay Ashok Thorat , Pravin Shridhar Wakte
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, India

ABSTRACT

Aim: To develop and validate a simple, precise, accurate, and sensitive UV-visible spectrophotometric method for the simultaneous estimation of Nelfinavir and Quercetin in patented pharmaceutical formulation and bulk by using a first-order derivative spectroscopic method according to the ICH guidelines. Methods: The zero-order spectra were obtained over the range of 200-400 nm for different concentrations of Nelfinavir and Quercetin, the overlay was converted to the first-order derivative spectra and zero-crossing points were selected as working wavelengths. Multiple calibration standards of both drugs were prepared separately and absorbance values were recorded at respective wavelengths. The proposed analytical method was validated according to ICH guidelines and various parameters viz. accuracy, precision, Limit of Detection, Limit of Quantitation, ruggedness, and robustness were assessed. Results: The zero-crossing points of Nelfinavir and Quercetin were found to be 284 nm and 371 nm respectively. The linearity range was found to be between 10-60 μg/ml for Nelfinavir and 02-10 μg/ml for Quercetin with a correlation coefficient of 0.999. The intra-and inter-day precision study showed a percent relative standard deviation between 0.44-1.44 and 0.21-1.02 respectively. LOD and LOQ of the proposed method were found to be 0.1195 μg/ml and 0.3623 μg/ml for Nelfinavir respectively whereas for Quercetin, said values were 0.0372μg/ml and 0.1129 μg/ml respectively. The total percent recovery of Nelfinavir and Quercetin was found to be in the range of 99.86 to 100.02 and 99.71 to 101.57 respectively. Conclusion: The simple, precise, accurate, and sensitive UV-visible spectrophotometric method for the simultaneous estimation of Nelfinavir and Quercetin in patented pharmaceutical formulation and bulk was developed and validated.

KEYWORDS
UV-Visible Spectrophotometric Method, A First-Order Derivative Spectrophotometric Method, Nelfinavir and Quercetin

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Sachin Shivling Bhusari , Akshay Ashok Thorat , Pravin Shridhar Wakte , "Simultaneous UV-Visible Spectrophotometric Determination of Nelfinavir and Quercetin in Patented Pharmaceutical Formulation and Bulk by First-Order Derivative Spectroscopic Method," Advances in Pharmacology and Pharmacy, Vol. 12, No. 2, pp. 95 - 103, 2024. DOI: 10.13189/app.2024.120202.

(b). APA Format:
Sachin Shivling Bhusari , Akshay Ashok Thorat , Pravin Shridhar Wakte (2024). Simultaneous UV-Visible Spectrophotometric Determination of Nelfinavir and Quercetin in Patented Pharmaceutical Formulation and Bulk by First-Order Derivative Spectroscopic Method. Advances in Pharmacology and Pharmacy, 12(2), 95 - 103. DOI: 10.13189/app.2024.120202.