Heterologous expression and characterization of a MoAA16 polysaccharide monooxygenase from the rice blast fungus Magnaporthe oryzae
Keywords
Cellulose
Enzymatic activity
Fungal infection
Heterologous expression
Magnaporthe oryzae
Monosaccharide
Oxidative cleavage
Pichia pastoris
Polysaccharide monooxygenase
Protein expression
Rice blast fungus
AbbreviationsBMGY
Buffered glycerol-complex medium
BMMY
Buffered methanol-complex medium
HRP
Horseradish peroxidase substrate
MCS
Multiple cloning sites
MWCO
Molecular weight cutoff
Ni-NTA
Nickel 2+ ion that has been coupled to Nitrilotriacetat acid (NTA)
PBST
Phosphate buffered saline – tween
YPD
Yeast extract peptone dextrose
Data availability
All Data and Materials are present in the main paper.
© 2023 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V
留言 (0)