Formation of osteoconductive biograft with bioorganic scaffold, human mesenchymal stromal cells, and platelet rich plasma with its evaluation in vitro

Keywords

Allogeneic platelet rich plasma

Biocompatibility

Biograft

Bioorganic scaffold

Bone reconstructive surgery

Cytotoxicity

Human bone marrow mesenchymal stromal cells

Osteoconductive biograft

Osteogenic differentiation

Proliferation

Regenerative medicine

Scaffolds

List of abbreviationsalPRP

allogeneic platelet rich plasma

AT-MSCs

mesenchymal stromal cells from adipose tissue

BMP2

bone morphogenetic protein 2

CCM

complete culture medium

dNTP

deoxyribonucleotide triphosphate

DPBS

dulbecco phosphate buffered saline

EDTA

ethylenediaminetetraacetic acid

ELISA

enzyme-linked immunosorbent assay

FGF

fibroblast growth factor

GapDH

glyceraldehyde-3-phosphate dehydrogenase

IGF-1

insulin-like growth factor 1

LBM

lyophilized bone matrix

MSCs

mesenchymal stromal cells

MTT

methyltetrazolium test

PDGF

platelet-derived growth factors

RT-qPCR

reverse transcription quantitative real-time polymerase chain reaction

RUNX2

runt-associated transcription factors 2

sGAGs

sulfated glycosaminoglycans

SVF-AT

stromal-vascular fraction of adipose tissue

TGF-β

transforming growth factor beta

VEGF

vascular endothelial growth factors

α-MEM

α-modified Eagle’s medium

Data availability

Data will be made available on request.

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