Gymnemic acid is glycosides of triterpene with recognized and valuable applications for several chronic diseases, mainly diabetics. Despite this, it requires a delivery system in order to range its therapeutic target due to its limited solubility and bioavailability. Therefore, the Gymnemic acid mediated gold nanoparticles ([email protected]) was synthesised by eco-friendly approach. The synthesised [email protected] was confirmed by the colour change from light yellow to a deep ruby red. UV − visible spectroscopy results showed a strong narrow peak at 530 nm, confirming the controlled synthesis of monodispersed [email protected] The reduction potential of standard Gymnemic acid (Gym) on synthesis of [email protected] was confirmed by using HPLC analysis. The spherical shaped [email protected] was observed by FESEM and HR-TEM studies with average size of 48.52 ± 5.53 nm. The XRD analysis exhibited a face-centered cubic (FCC) crystalline nature of [email protected] The in vivo antidiabetic activity of Gym and [email protected] were validated using Streptozotocin induced diabetic Albino wistar rats. The [email protected] and Gym were regulates the glucose and lipid levels in experimental animals. The histopathology outcomes shown that the [email protected] were restoration of pancreatic islets cells in the animals. This investigation demonstrated that the [email protected] had the potential anti-diabetic properties.