Skin wound healing, especially for chronic sores like diabetic foot ulcers, is still a medical emergency. Despite the abundance of treatment techniques that are currently accessible, none appear to be both effective and secure. In this context, we have emphasized the benefits of using clay and biopolymers to create nano-drug delivery systems. Pectin was thiolated (TFP) followed by the formulation of neomycin sulphate nanocomposites using polyethylene glycol and montmorillonite (PEG/MMT) to accelerate wound healing. Box-Behnken design optimized Nano composite preparation by considering concentrations of TFP, PEG and MMT as process variables. Based on the desirability approach (d=1), formulation containing coded values of MMT-0.91; PEG-0.99, and TFP of -0.71 can achieve the prerequisites of the optimum formulation. Optimized nanocomposite formulation was further formulated into nanocomposite film (ONCF) and evaluated for mechanical properties, drug release, cytotoxic, and in vitro wound healing capabilities. OCNF had no cytotoxic effects, as evident from the more than 90% survival rate of C6 glioma cells. In vitro wound, healing activities prove that composite-to-film conversion will be responsible for most antioxidant activity. In vivo wound healing and histomorphometry studies demonstrate the formulation's efficacy in wound healing. Therefore, the TFP-PEG/MMT nanocomposite film is of great potential in the biomedical field.