St Sauver, J. L. et al. Why patients visit their doctors: assessing the most prevalent conditions in a defined American population. Mayo Clin. Proc. 88, 56–67 (2013).
Sharif, B., Ase, A. R., Ribeiro-da-Silva, A. & Seguela, P. Differential coding of itch and pain by a subpopulation of primary afferent neurons. Neuron 106, 940–951 (2020).
Article CAS PubMed Google Scholar
Racine, M., Hudson, M., Baron, M., Nielson, W. R. & Canadian Scleroderma Research, G. The impact of pain and itch on functioning and health-related quality of life in systemic sclerosis: an exploratory study. J. Pain. Symptom Manag. 52, 43–53 (2016).
Klein, A. et al. Pruriception and neuronal coding in nociceptor subtypes in human and nonhuman primates. eLife 10, e64506 (2021).
Sun, S. et al. Leaky gate model: intensity-dependent coding of pain and itch in the spinal cord. Neuron 93, 840–853 (2017).
Article CAS PubMed PubMed Central Google Scholar
Shim, W. S. & Oh, U. Histamine-induced itch and its relationship with pain. Mol. Pain. 4, 29 (2008).
Article PubMed PubMed Central Google Scholar
Meixiong, J. & Dong, X. Mas-related G protein–coupled receptors and the biology of itch sensation. Annu. Rev. Genet. 51, 103–121 (2017).
Article CAS PubMed Google Scholar
Akiyama, T., Lerner, E. A. & Carstens, E. Protease-activated receptors and itch. Handb. Exp. Pharmacol. 226, 219–235 (2015).
Article CAS PubMed PubMed Central Google Scholar
Feng, J. et al. Piezo2 channel–Merkel cell signaling modulates the conversion of touch to itch. Science 360, 530–533 (2018).
Article CAS PubMed PubMed Central Google Scholar
Dong, X., Han, S., Zylka, M. J., Simon, M. I. & Anderson, D. J. A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons. Cell 106, 619–632 (2001).
Article CAS PubMed Google Scholar
Liu, Q. et al. Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus. Cell 139, 1353–1365 (2009).
Article PubMed PubMed Central Google Scholar
Li, Z. et al. Targeting human Mas-related G protein–coupled receptor X1 to inhibit persistent pain. Proc. Natl Acad. Sci. USA 114, E1996–E2005 (2017).
CAS PubMed PubMed Central Google Scholar
Lembo, P. M. et al. Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs. Nat. Neurosci. 5, 201–209 (2002).
Article CAS PubMed Google Scholar
Wen, W. et al. Discovery and characterization of 2-(cyclopropanesulfonamido)-N-(2-ethoxyphenyl)benzamide, ML382: a potent and selective positive allosteric modulator of MrgX1. ChemMedChem 10, 57–61 (2015).
Article CAS PubMed Google Scholar
Tseng, P. Y., Zheng, Q., Li, Z. & Dong, X. MrgprX1 mediates neuronal excitability and itch through tetrodotoxin-resistant sodium channels. Itch (Phila) 4, e28 (2019).
Cao, C. et al. Structure, function and pharmacology of human itch GPCRs. Nature 600, 170–175 (2021).
Article CAS PubMed PubMed Central Google Scholar
Prchalová, E. et al. Discovery of benzamidine- and 1-aminoisoquinoline-based human MAS-related G-protein-coupled receptor X1 (MRGPRX1) agonists. J. Med. Chem. 62, 8631–8641 (2019).
Kim, K. et al. Structure of a hallucinogen-activated Gq-coupled 5-HT2A serotonin receptor. Cell 182, 1574–1588 (2020).
Article CAS PubMed PubMed Central Google Scholar
Koehl, A. et al. Structure of the µ-opioid receptor–Gi protein complex. Nature 558, 547–552 (2018).
Ballesteros, J. A. & Weinstein, H. in Methods in Neurosciences Vol. 25 (ed Sealfon, S. C.) 366–428 (Academic Press, 1995).
Han, S. K. et al. Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Gαq/11 pathway. Proc. Natl Acad. Sci. USA 99, 14740–14745 (2002).
Article CAS PubMed PubMed Central Google Scholar
Olsen, R. H. J. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol. 16, 841–849 (2020).
Article CAS PubMed PubMed Central Google Scholar
Kruse, A. C. et al. Activation and allosteric modulation of a muscarinic acetylcholine receptor. Nature 504, 101–106 (2013).
Article CAS PubMed PubMed Central Google Scholar
Wold, E. A., Chen, J., Cunningham, K. A. & Zhou, J. Allosteric modulation of class A GPCRs: targets, agents, and emerging concepts. J. Med. Chem. 62, 88–127 (2019).
Article CAS PubMed Google Scholar
Lu, J. et al. Structural basis for the cooperative allosteric activation of the free fatty acid receptor GPR40. Nat. Struct. Mol. Biol. 24, 570–577 (2017).
Article CAS PubMed Google Scholar
Zhuang, Y. et al. Mechanism of dopamine binding and allosteric modulation of the human D1 dopamine receptor. Cell Res. 31, 593–596 (2021).
Article CAS PubMed PubMed Central Google Scholar
Karhu, T. et al. Isolation of new ligands for orphan receptor MRGPRX1—hemorphins LVV-H7 and VV-H7. Peptides 96, 61–66 (2017).
Article CAS PubMed Google Scholar
Li, X. et al. Tick peptides evoke itch by activating MrgprC11/MRGPRX1 to sensitize TRPV1 in pruriceptors. J. Allergy Clin. Immunol. 147, 2236–2248 (2021).
Article CAS PubMed Google Scholar
Du, Y. et al. Assembly of a GPCR-G protein complex. Cell 177, 1232–1242 (2019).
Article CAS PubMed PubMed Central Google Scholar
Xu, P. et al. Structural insights into the lipid and ligand regulation of serotonin receptors. Nature 592, 469–473 (2021).
Article CAS PubMed Google Scholar
Peck, J. V., Fay, J. F. & Strauss, J. D. High-speed high-resolution data collection on a 200 keV cryo-TEM. IUCrJ 9, 243–252 (2022).
Article CAS PubMed PubMed Central Google Scholar
Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290–296 (2017).
Article CAS PubMed Google Scholar
Punjani, A., Zhang, H. & Fleet, D. J. Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction. Nat. Methods 17, 1214–1221 (2020).
Article CAS PubMed Google Scholar
Rosenthal, P. B. & Henderson, R. Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. J. Mol. Biol. 333, 721–745 (2003).
Article CAS PubMed Google Scholar
Sanchez-Garcia, R. et al. DeepEMhancer: a deep learning solution for cryo-EM volume post-processing. Commun. Biol. 4, 874 (2021).
Article PubMed PubMed Central Google Scholar
Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).
Article CAS PubMed PubMed Central Google Scholar
Pettersen, E. F. et al. UCSF Chimera—a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004).
Article CAS PubMed Google Scholar
Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D Biol. Crystallogr. 60, 2126–2132 (2004).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213–221 (2010).
Article CAS PubMed PubMed Central Google Scholar
Robertson, M. J., van Zundert, G. C. P., Borrelli, K. & Skiniotis, G. GemSpot: a pipeline for robust modeling of ligands into cryo-EM maps. Structure 28, 707–716 (2020).
留言 (0)