Introduction

Iron plays a fundamental role in maintaining several biological functions in the brain. In the brain, iron is essential for meeting the high metabolic and energy demands of neuronal tissue and is also involved in myelin synthesis, neurotransmitter synthesis and metabolism (Gerlach et al., 1994; Belaidi and Bush, 2016). Iron dyshomeostasis within the brain can cause oxidative stress and inflammatory responses, causing neurotoxicity and cell damage and ultimately neurological diseases (Ward et al., 2014).

Ferroptosis is a new type of programmed cell death identified in recent years that is different from other cell death forms, such as apoptosis, necrosis, autophagy or pyroptosis. Ferroptosis is induced by the iron-dependent accumulation of lipid peroxides, and reactive oxygen species (ROS) production, the depletion of glutathione (GSH), and inactivation of glutathione peroxidase 4 (GPX4) in cells (Dixon et al., 2012; Chen et al., 2021a).

Accumulating evidence indicates that Alzheimer’s disease (AD) pathology is closely related to iron dyshomeostasis and ferroptosis. Studies show that brain iron accumulation is more serious in special brain areas of patients with neurodegenerative diseases and induces oxidative stress and neuronal death. Iron metabolism imbalance in the brain and dysfunction of endogenous antioxidant systems, including GPX, are closely related to the etiopathogenesis of AD (Hambright et al., 2017). Patients with mild cognitive impairment (MCI) and a high Aβ plaque load exhibit higher cortical iron accumulation, which increases the risk of AD (van Bergen et al., 2016). Evidence of elevated iron and the production of lipid peroxidation in the AD brain indicates the important role of ferroptosis in the pathogenesis of AD. The features of ferroptosis can be detected in the brain tissues of AD patients and mouse models, such as abnormal iron accumulation, glutamate excitotoxicity and lipid peroxidation accumulation (Lyras et al., 1997; Huang et al., 2017; Ayton et al., 2021). Evidence has shown that ferritin levels in cerebrospinal fluid (CSF) are negatively associated with MCI and AD patients. Ferritin was strongly associated with CSF apolipoprotein E levels and was elevated by the Alzheimer’s risk allele APOE-ɛ4, indicating that iron imbalance can be one of the risk factors for AD. Iron dyshomeostasis and ferroptosis may play the important roles in neuronal death in AD (Ayton et al., 2015).

Characteristics of ferroptosis in AD

The classic features of ferroptosis include the following: 1) specific changes in cellular morphology including a ferroptosis-triggered decrease in mitochondrial size, an increase in membrane density and a decrease in cristae number without obvious morphological changes in the nucleus and 2) specific changes in cellular composition including iron-promoted increases in lipid peroxides and ROS levels, depletion of GSH, inactivation of GPX4, and changes in the expression of several regulated genes (Dixon et al., 2012; Chen et al., 2021a).

Iron metabolism is involved in ferroptosis in neuron cells

The balance of iron metabolism depends on the synergistic action of various iron metabolism-related proteins, and iron dyshomeostasis may lead to ferroptosis. The transferrin (Tf)/transferrin receptor (TfR) pathway is mainly responsible for the absorption of iron into cells. When the iron transporter function of the Tf/TfR pathway is abnormal, it will lead to oxidative stress and ferroptosis. Abnormal Tf and TfR1 expression can lead to neurodegenerative disorders (Senyilmaz et al., 2015). Studies have shown that overexpression of divalent metal ion transporter 1 (DMT1), as an iron-absorbing protein, leads to the iron deposition in substantia nigra and death of dopaminergic neurons (Zhang et al., 2017). Ferritin is the main intracellular iron storage protein composed of light chain protein and heavy chain protein, which stores iron in a redox inactive form and protects cells and tissues from oxidative damage. Iron dyshomeostasis caused by abnormal ferritin will induce ferroptosis (Park and Chung, 2019). Upon ferroptosis, ferritin in the cytoplasm undergoes ferritinophagy in the lysosome to further expand labile iron pool (LIP), and ferritinophagy enhances cysteine deficiency-induced ferroptosis. While total intracellular iron content may not change, LIP expansion predisposes cells to ferroptosis. Imbalances in iron metabolism lead to iron overload, which in turn generates free radicals through the Fenton and Haber-Weiss reactions, thereby activating ferroptosis (Kajarabille and Latunde-Dada, 2019).

GSH/GPX4 is involved in ferroptosis by mediating lipid peroxidation in neuron cells

Massive accumulation of lipid peroxides to lethal levels is a necessary signal for ferroptosis, and the levels of lipid peroxide biomarkers are also elevated in neuronal cells in AD. Due to the accumulation of iron, the Fenton and Haber-Weiss reaction occurs in cells, and a large amount of ROS is generated, leading to lipid peroxidation (Kajarabille and Latunde-Dada, 2019). The enzyme GSH plays a key role in protecting cells from oxidative damage, and GSH-dependent GPX4 activity is important for regulating neuronal ferroptosis. GPX4 utilizes the thiol groups of GSH as electron donors and affects the antioxidant response of neuron cells. Therefore, GPX4 inactivation caused by GSH depletion increases intracellular lipid peroxidation and induces ferroptosis (Imai et al., 2017). Studies have shown that neuron-specific knockout of GPX4 leads to lipid peroxidation and the accumulation of intracellular ROS, eventually leading to neuronal death, suggesting that GPX4 may be a key regulator of ferroptosis in neuron cells (Hambright et al., 2017).

In the current study, the occurrence of neuronal ferroptosis was determined by evaluating morphological changes or pharmacological and molecular characteristics. However, changes in the levels of intracellular iron metabolism-related proteins and lipid peroxidation factors including downregulation of GSH and GPX4 and upregulation of lipid peroxidation markers such as MDA, isoprostanes, 4-HNE, malondialdehyde and acrolein are key markers of neuronal ferroptosis (Gegotek and Skrzydlewska, 2019).

The involvement of novel ferroptosis-related genes in AD was revealed by advanced biological sequencing methods. For example, Wang et al. found five hub genes (JUN, SLC2A1, TFRC, ALB, and NFE2L2) that are closely associated with ferroptosis in AD and can differentiate AD patients from controls; these genes can be used as potential ferroptosis-related biomarkers for disease diagnosis and therapeutic monitoring (Wang et al., 2022).

Mechanisms of iron dyshomeostasis and ferroptosis leading to ADIron dyshomeostasis contribute to senile plaques deposition and neurofibrillary tangles

Iron is an endogenous metal ion involved in many important physiological processes in the brain. Under physiological conditions, iron ions are absorbed, transported, transformed and excreted by the human body to maintain their normal levels. Once free iron ions are enriched, cells will produce excess ROS, leading to ferroptosis. Therefore, the increase in iron ion content may be one of the critical factors for AD pathogenesis. Studies have shown that abnormal iron metabolism is related to the pathogenesis of AD. Compared with controls, AD patients had elevated iron levels in the hippocampus, cortex, and basal ganglia regions of the brain and lower levels of iron in the cerebral cortex, brainstem, and cerebellum (Ramos et al., 2014). The iron content and ferritin protein expression in brain tissue were related to the degree of amyloid deposition, due to the level of APP, like ferritin, can be regulated by iron content at the translational level. Thus, the increased levels of iron content in the brain might affect the progression of AD (Rogers et al., 2008; Chen et al., 2019; Svobodova et al., 2019). A study by Rogers et al. showed that AD patients receiving intramuscular injection of the iron chelator deferoxamine (DFO) had a lesser degree of decline in activities of daily living than AD patients receiving placebo, indicating that abnormal iron metabolism can affect the progression of AD (Crapper McLachlan et al., 1991).

Accumulating evidence suggests that iron dyshomeostasis contribute to the aggregation of beta-amyloid (Aβ)-induced SP deposition and hyperphosphorylated tau proteins that form NFTs in the brain. SP and NFTs cover redox-active transition metals.

Iron dyshomeostasis induces Aβ level elevation

Aβ formation results from the amyloidogenic cleavage of human amyloid precursor protein (APP). APP is processed by two different pathways: the a- and γ-secretase-mediated nonamyloid protein production pathway and the β- and γ-secretase-mediated amyloid protein production pathway (O'Brien and Wong, 2011). APP is a type 1 transmembrane glycoprotein and a key precursor for the production of Aβ. APP can be sequentially cleaved by a-secretase or β-secretase first and then by the γ-secretase complex. Under normal physiological conditions, APP can be cleaved by a-secretase within the Aβ domain to release soluble APPα and preclude Aβ generation. However, once APP is cleaved first by β-secretase and then by the γ-secretase complex, it will eventually produce neurotoxic amyloid of 40–42 amino acids. The 5′-untranslated region (5′-UTR mRNA) of APP mRNA contains iron-responsive elements (IREs), which is regulated by intracellular iron content (Figure 1A). APP can be posttranscriptionally regulated by iron‐responsive‐element‐binding proteins (IRPs)-IREs. Under conditions of insufficient iron, IRPs bind tightly to IREs at the 5′-UTR site of APP mRNA and repress APP translation. During intracellular iron overload, iron can bind to IRPs, leading to the dissociation of IRPs from IREs and promoting the translation of APP, thereby increasing the level of APP protein and possibly producing excess Aβ. However, in turn, APP can interact with and stabilize ferroportin (Fpn) to facilitate iron export and maintain the homeostasis of iron concentration (Belaidi et al., 2018).

FIGURE 1

FIGURE 1. Iron dyshomeostasis contribute to senile plaques deposition and neurofibrillary tangles. (A) The 5′-UTR of APP mRNA contains IREs. APP can be posttranscriptionally regulated by IRP-IRE. Under conditions of insufficient iron, IRPs bind to IREs of APP mRNA and repress APP translation. During iron overload, iron can bind to IRPs, leading to the dissociation of IRPs from IREs and promoting the translation of APP. (B) NRF2, as a negative regulator of BACE1, represses the expression of BACE1 by binding to AREs. Nrf2 activation repress BACE1 transcription. In turn, NRF2 inactivation promote BACE1 transcription. (C) Increased cellular iron levels promote the activity of enzymes such as GSK3β, CDK5, and MAPK, which promote tau hyperphosphorylation. Fe3+ can promote the formation of tau fibrils by binding to the phosphorylated tau.

Iron also affects a-secretase activity and suppresses the nonamyloidogenic pathway, thereby increasing Aβ production through the amyloidogenic pathway. Furin plays a critical role in mediating APP processing by regulating the activation of a-secretase. Furin is a proprotein convertase enzyme that converts various proproteins to mature proteins, which are affected by iron content. Wichaiyo et al. demonstrated that iron affected the expression of furin by posttranslational regulation. In the iron overload condition, furin mRNA showed a significant reduction (Wichaiyo et al., 2015). Furin is an important regulator of a-secretase-associated APP processing (Hwang et al., 2006). Hwang et al. demonstrated that the levels of furin in the brains of AD patients and Tg2576 mice were significantly reduced, yet injection of furin-adenovirus into Tg2576 mouse brains markedly increased a-secretase activity and reduced Aβ production in infected brain regions. The researchers proved that furin enhances a-secretase activity via the cleavage of ADAM10 and TACE to change to the mature forms and promotes sAPPa secretion to suppress Aβ generation.

In addition, nuclear factor erythroid 2-related factor 2 (NRF2) as a critical regulator related to ferroptosis play an important role in the development of AD. NRF2 is a transcription factor that can regulate a considerable number of genes, including genes related to iron and glutathione metabolism, which are associated with ferroptosis. NRF2 is activated to resist oxidative stress induced by excessive iron to protect tissues and cells from ferroptosis (Lim et al., 2019). The reduced levels of Nrf2 mRNA in the brains of AD patients also indicate that ferroptosis participates in the pathological process of AD. Recently reported that NRF2 as a negative regulator of BACE1, represses the expression of BACE1 by binding to antioxidant response elements (AREs) (Figure 1B). In AD patients and animal models, the mRNA levels of Nrf2 were significantly reduced, and Nrf2 deficiency accelerated BACE1 expression and Aβ production (Bahn et al., 2019). Drugs, such as spinosin, can suppress BACE1 expression by promoting Nrf2 expression and the activation of the Nrf2/HO-1 pathway, thereby inhibiting Aβ1-42 production (Zhang et al., 2020). In addition, iron also directly binds to the His6, His13 and His14 amino acid residues of Aβ, thereby enhancing the neurotoxicity of Aβ (Azimi and Rauk, 2012).

Iron overload-induced tau hyperphosphorylation and NFT formation

Emerging evidence has demonstrated that there is a close connection between the abnormal tau pathology and the accumulation of iron contents in the brains of individuals with neurodegenerative disorders (Rao and Adlard, 2018). Dysregulation of brain iron homeostasis is closely related to tau hyperphosphorylation and the NFTs formation. Studies have shown that in the cortex and hippocampus of AD, NFTs accumulate with increasing iron levels in the brain regions (Spotorno et al., 2020). Tau hyperphosphorylation is a critical step for NFT formation, which is one of the important factors correlated with AD pathology (Duce et al., 2010). Tau hyperphosphorylation is regulated by the aberrant activation of tau kinases such as glycogen synthase kinase-3β (GSK3β), cyclin-dependent protein kinase-5 (CDK5) and mitogen-activated protein kinases (MAPKs). Increased cellular iron levels promote the activity of multiple kinases, such as GSK3β, CDK5, MAPK and protein phosphatase 2A, which promote tau hyperphosphorylation (Hall et al., 2011) (Figure 1C). However, intranasal DFO treatment can inhibit iron-induced tau phosphorylation via the CDK5 and GSK-3 pathways in APP/PS1 double transgenic mice (Guo et al., 2013).

In addition, the oxidation states of iron are closely related to the aggregation states of tau hyperphosphorylation. Fe3+ can promote the aggregation of hyperphosphorylated tau by binding to His residues of Tau (Figure 1C). However, Fe2+ may mediate reversible conformational changes of aggregation by binding to Thr residues of tau. Iron may function as a cofactor for tau aggregation, which causes aggregation of hyperphosphorylated tau via an iron-binding motif in the tau protein (Rao and Adlard, 2018).

Conversely, tau dysregulation also induced iron dyshomeostasis. Studies have shown that knockdown of Tau induce the accumulation of iron in the cortex, hippocampus and substantia nigra of Tau-KO mice, leading to age-dependent neurodegeneration (Lei et al., 2012). This is because iron export is mediated mainly through the ferroportin (Fpn) interaction with APP in neurons. The dysfunction of tau leads to impaired transport of APP to the membrane, which in turn affects the interaction of APP with Fpn to repress the export of iron from the cell, resulting in iron retention (Lei et al., 2012).

Dysfunction of system Xc-induce excitotoxic neuronal degeneration in AD

The cystine/glutamate antiporter system (system Xc-), composed of the light chain SLC7A11 and the heavy chain SLC3A2, is an important glutamate transporter in the central nervous system (CNS) for the cellular uptake of cystine in exchange for intracellular glutamate. It mainly functions to mediate cellular cystine uptake for the synthesis of GSH, which is essential for cellular protection from oxidative stress. Glutamate is an important excitatory transmitter in the CNS and is critical for maintaining normal brain function and CNS development. However, glutamate levels can affect the function of system Xc-. High extracellular concentrations of glutamate disrupt system Xc- and thus induce ferroptosis.

The antioxidant properties of system Xc-are mainly achieved by increasing the synthesis of GSH. Cysteine is an important precursor in the GSH synthesis process and functions as a rate-limiting factor in the synthesis process. Studies have shown that cysteine in plasma and cerebrospinal fluid is present at a very low concentration, which is easily oxidized, and the ability to synthesize cysteine in cells is limited. Therefore, the maintenance of intracellular cysteine is mainly through the transport of cystine into the cell, where it is reduced to cysteine immediately (Tu et al., 2021). Cystine transported into astrocytes or neurons relies on system Xc-, which drives the cysteine/cystine redox cycle and protects cells from oxidative stress (Banjac et al., 2008; Lewerenz et al., 2012). However, the transport of cystine into the cell through system Xc-is accompanied by the release of a large amount of glutamate, which activates the NMDA receptors of neurons to affect the transmission of excitatory messages. Dysfunction of system Xc-not only leads to oxidative damage but also causes the release of glutamate and an increase in the extracellular glutamate concentration, resulting in neuronal glutamate excitotoxicity. In particular, the pharmacological inhibition of system Xc-by certain small molecule compounds or drugs (for example, erastin, sulfasalazine, and sorafenib) can trigger lipid peroxidation and ferroptosis.

System Xc-in microglia or astrocytes might be involved in Aβ neurotoxicity (Figure 2). Aβ produced by neurons aggregates extracellularly to form toxic Aβ aggregates, which diffuse and exert their toxic effects on the surrounding cells, contributing to oxidative stress and neuroinflammation in AD. Microglia or astrocytes import l-cystine into the cell through system Xc-to synthesize GSH and resist oxidative stress induced by Aβ while simultaneously releasing glutamate. However, microglia and astrocytes in turn enhance the toxicity of Aβ to neurons by releasing excessive glutamate through system Xc-to provoke excitotoxicity. For example, D'Ezio et al. revealed that Aβ25-35 induced neurotoxicity by increased glutamate release was due to the increased expression of system Xc-in astrocytes. Aβ25-35 triggers an antioxidant response in astrocytes by inducing the activation of the Nrf2/ARE pathway and the upregulation of System Xc-. However, the upregulation of system Xc-can increase extracellular glutamate release and potentially cause excitotoxicity (D'Ezio et al., 2021). Blocking system Xc-not only protected microglia from the cytotoxicity of Aβ but also protected neurons from the excitotoxicity caused by the release of glutamate from microglia (Qin et al., 2006).

FIGURE 2

FIGURE 2. Dysfunction of system Xc-induce excitotoxic neuronal degeneration in AD. Aβ produced by neurons aggregates extracellularly to form toxic Aβ aggregates causes oxidative stress in microglia or astrocytes, which activates Nrf2 and produces SOD to resist oxidative stress. The activation of Nrf2 also can upregulate system Xc-to resist oxidative stress induced by Aβ while simultaneously releasing glutamate. However, excessively released glutamate can cause excitotoxicity in neurons, leading to cell death.

GSH/GPX4 pathway in AD

Emerging evidence has identified GPX4 as one of the key regulators of ferroptosis in nervous cells in the brain. GPX4 is a selenoprotein glutathione peroxidase that has been proven to repair lipid hydroperoxides in membranes. Lipid peroxidation is one feature of ferroptosis-induced cell death. GPX4 functions as a repressor of 12/15-lipoxygenase-derived peroxidation, which can detoxify hydroperoxides in membrane lipids directly, thereby reducing damage to membrane function and preventing the generation of lipid peroxidation-derived reactive products such as 4-hydroxynonenal (4-HNE). GPX4 can reduce direct toxic lipid peroxide (PLOOH) to nontoxic lipid alcohols (PL-OH) using GSH as a cofactor (Imai et al., 2017). GPX4 was reported to be a regulator of ferroptosis by RSL3 and erastin, since RSL3 and erastin decrease the activity of GPX4 by directly binding to GPX4 and indirectly losing glutathione. GPX4 overexpression suppresses RSL3-induced ferroptosis, whereas GPX4 knockdown enhances the sensitivity to RSL3-induced ferroptosis, suggesting that the inhibition of GPX4 activity is a major contributor to ferroptosis.

GPX4 was identified to have reduced expression in AD. Suppression of GPX4 is one of the critical factors for ferroptosis-related AD pathogenesis. Knockout of GPX4 in the brain causes a series of features associated with AD pathology, such as cognitive impairment and hippocampal neurodegeneration in Gpx4BIKO mice (Hambright et al., 2017). Moreover, classical features closely related to ferroptosis, such as elevated lipid peroxidation, were also observed in these brain regions, and treatment with ferroptosis inhibitors improved cognitive impairment and neurodegeneration in these mice.

In addition, studies have shown that GPX4 deletion upregulates β-secretase activity and leads to elevated Aβ production. Chen et al. revealed that Gpx4+/−mice had higher levels of β-secretase activity elevated by increased lipid peroxidation, which resulted in the increased expression of BACE1 at the protein level (Chen et al., 2008). As previous studies have revealed, Aβ plaques and lipid peroxidation products were colocalized in the brains of AD patients. The high level of BACE1 protein led to increased amyloid plaque burdens and increased Aβ1–40 and Aβ1–42 levels in middle-aged Gpx4+/−mice. Therefore, Aβ peptides led to lipid peroxidation in AD. For example, lipid oxidation enzymes that catalyze the deoxygenation of PUFAs, such as lipoxygenase (LOX), cyclooxygenases (COXs), and cytochrome p450 (CYPs), have changed in AD pathology (Wu et al., 2018). In turn, lipid peroxidation products increase APP processing and Aβ production through the upregulation of BACE1.

Dysfunction of NCOA4-mediated ferritinophagy induced ferroptosis in AD

Ferritinophagy is mediated by NCOA4, which releases free iron by the autophagic degradation of ferritin (Santana-Codina et al., 2021). NCOA4, as a selective autophagy receptor, has been identified to colocalize with endogenous LC3B and intracellular iron storage ferritin complexes. Ferritinophagy plays an important role in the physiological and pathological processes of cell growth, proliferation, differentiation, apoptosis and carcinogenesis. Some evidence has demonstrated that ferritinophagy-mediated ferroptosis is one of the critical mechanisms contributing to neurodegenerative diseases, such as AD (Quiles Del Rey and Mancias, 2019). Ferritinophagy is regulated by NCOA4, an iron-dependent protein, to maintain intracellular iron balance and exert the normal physiological functions of iron. However, excessive activation of ferritinophagy causes ferroptosis cell death. Ferroptosis can be initiated by ferritinophagy through promoting iron and ROS accumulation.

Ferritin is an iron storage protein composed of 24 subunits of two types, ferritin light chain (FTL) and heavy chain (FTH), which play an important role in maintaining iron homeostasis. When cellular iron is insufficient, ferritin-containing iron combines with NCOA4 to form a complex, which mediates iron release from ferritin storage through the ferritinophagy pathway (Santana-Codina et al., 2021). NCOA4 selectively binds to the FTH1 subunit of ferritin, and the conserved surface arginine (R23) on FTH1 is an important region that interacts with the C-terminal domain of NCOA4. When cellular iron is overloaded, the binding of NCOA4 and HERC2 (HECT and RLD domain containing E3 ubiquitin protein ligase 2) is enhanced. HERC2 is an E3 ubiquitin ligase composed of multiple structural domains that can regulate NCOA4 levels through the ubiquitin–proteasome system. The binding region of HERC2 and NCOA4 are the same as the binding region of NCOA4 and ferritin. HERC2 mediates the degradation of NCOA4 in an ubiquitin-dependent manner through the CUL7 homology domain of HERC2 and the C-terminal domain of NCOA4, thereby suppressing the binding of NCOA4 and ferritin and inhibiting the release of iron and ferritin degradation by ferritinophagy. When the available cellular iron decreases, the binding of NCOA4 to HERC2 is weakened, and NCOA4 binds to ferritin and is transported to autophagosomes, causing ferritinophagy to degrade ferritin and release free iron Fe2+. However, the excessive Fe2+ content in cells transforms Fe2+ into Fe3+ through the Fenton reaction to generate ROS, which causes lipid peroxidation and oxidative stress and ultimately leads to ferroptosis (Figure 3).

FIGURE 3

FIGURE 3. Dysfunction of NCOA4-mediated ferritinophagy induced ferroptosis in AD. NCOA4 can release free iron by the autophagic degradation of ferritin. When cellular iron is overloaded, the binding of NCOA4 and HERC2 is enhanced. HERC2 mediates the degradation of NCOA4 in an ubiquitin-dependent manner, thereby suppressing the binding of NCOA4 and ferritin and inhibiting the release of iron and ferritin degradation by ferritinophagy. When cellular iron is insufficient, ferritin-containing iron combines with NCOA4 to form a complex through FTH1 subunit of ferritin, which mediates iron release from ferritin storage through the ferritinophagy pathway. However, the excessive Fe2+ content in cells transforms Fe2+ into Fe3+ through the Fenton reaction to generate ROS, which causes lipid peroxidation and oxidative stress and ultimately leads to ferroptosis.

In addition, ferritinophagy dysfunction might affect the level of iron metabolism-related proteins, leading to excessive iron storage in the cells and causing ferroptosis (Santana-Codina et al., 2021). Iron is transported throughout the circulatory system by binding to Tf. Through the binding of the iron-Tf complex to the TfR, iron is then transported from serum to cells. When the depletion of NCOA4 leads to impaired ferritinophagy, the availability of cellular iron is reduced, whereas IRP2 activity is induced to bind to the IREs in the 5′-UTR of ferritin mRNA and the 3′-UTR of TfR mRNA. This inhibits the translation of ferritin mRNA yet stabilizes the TfR mRNA to promote iron transport into cells and Fe2+ accumulation, finally inducing ferroptosis.

Major pathways regulating ferroptosis in ADModulating NRF2 to regulate ferroptosis

Nrf2 is a master transcription factor that is conducive to improving the state of oxidative stress in the body, promoting cell survival and maintaining cellular redox homeostasis. The expression of NRF2 decreases with age, which indicates that it may play a role in the development of AD by promoting sensitivity to ferroptotic stress. In addition, the levels of many NRF2-regulated proteins such as GSH biosynthesis enzymes, GPX4, NQO1 and iron metabolism-related proteins that are linked to ferroptosis have been shown to be altered in AD (Hambright et al., 2017; Lane et al., 2021).

Keap1/Nrf2/ARE pathway

Nrf2 belongs to the basic leucine zipper (bZIP) transcription factor family and retains a cap-n-collar (CNC) structure. The N-terminal region of Nrf2 has a Neh2 domain and contains DLG and ETGE motifs. Under normal physiological conditions, Nrf2 interacts with Keap1 and is anchored in the cytoplasm (Bellezza et al., 2018) (Figure 4).

FIGURE 4

FIGURE 4. Modulating NRF2 to regulate ferroptosis. Regulation of ferroptosis through transcriptional and posttranscriptional regulation of Nrf2 is important for AD pathophysiology, including Nrf2 expression, Nrf2 phosphorylation and acetylation/deacetylation.

Keap1 is a major intracellular regulator of Nrf2, which forms a homodimer to bind Cullin3 (Cul3), an adaptor to the Cul3-type E3 ubiquitin ligase complex, resulting in ubiquitination. Under normal physiological conditions, the Neh2 domain of Nrf2 binds to the DGR region of Keap1 to be anchored in the cytoplasm. Keap1 acts as a substrate for the Cul3-dependent E3 ubiquitin ligase complex (Cul3/Rbx1 E3 ubiquitin), which can promote the ubiquitination of Nrf2 and its rapid degradation by the proteasome to maintain Nrf2 levels. Under oxidative stress conditions, such as excessive ROS production, Nrf2 disconnects from Keap1 and migrates to the nucleus, where it can dimerize with small Maf family members and bind to ARE (Bellezza et al., 2018).

The ARE is defined as a cis-acting DNA enhancer motif and is positioned in the promoter of antioxidant genes. Binding of Nrf2 to ARE regulates the transcriptional activation of many important endogenous antioxidants (such as superoxide dismutase (SOD) and enhanced catalase (CAT)) and phase II detoxifying enzymes (such as NAD(P)H quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), glutathione S-transferase A1 (GSTA1), glutamate-cysteine ligase modifier subunit (GCLM) and catalytic subunit (GCLC)) to remove harmful molecules such as ROS (Buendia et al., 2016). HO-1 is one of the detoxifying enzymes in phase II, and the downstream signaling axis induced by it has a protective effect against oxidative stress in multiple organs. In addition, the expression of SLC7A11 and GPX4 is also transcriptionally regulated by Nrf2 (Yuan et al., 2021).

Nrf2 phosphorylation in regulating ferroptosis

The phosphorylation of Nrf2 is one of the modes by which Nrf2 nuclear accumulation and nuclear exclusion and degradation are regulated. Nrf2 comprises several sites for phosphorylation, which can be phosphorylated by many kinases (Figure 4). GSK-3β is a serine/threonine kinase that is hyperactive in the brains of AD patients. GSK-3β exerts a negative regulatory effect on Nrf2 activity by phosphorylation (Culbreth and Aschner, 2018). GSK-3β phosphorylates the Neh6 region of Nrf2 at serine residues 334–338, which is recognized by β-TrCP E3 ligase, resulting in ubiquitination and proteasomal degradation of Nrf2 (Rada et al., 2011). Activation of the PI3K/Akt pathway can indirectly regulate Nrf2 through the negative regulation of GSK-3β, while inactive GSK-3β increases Nrf2 stability and Nrf2-related gene expression. PI3K phosphorylates Akt at Ser473 to activate Akt. The phosphorylated Akt further phosphorylates GSK-3β at Ser9 and negatively regulates GSK-3β (inactivating GSK-3β), thereby suppressing GSK-3β from phosphorylating Nrf2 at Ser334-338 and promoting the activation of Nrf2 by translocation into the nucleus to initiate transcriptional activation of antioxidant enzymes (Bian et al., 2021). In addition, protein kinase C (PKC) is a serine/threonine kinase that can phosphorylate Nrf2 at Ser40 in the Neh2 domain, dissociate Nrf2 from Keap1, and promote the nuclear translocation of Nrf2, thus transcriptionally activating HO-1 expression (Huang et al., 2002; Fao et al., 2019). AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine enzyme. AMPK can directly phosphorylate Nrf2 at Ser558 (Ser550 in mouse), which together with AMPK-mediated GSK3β inactivation promotes nuclear accumulation of Nrf2 for ARE-driven gene transactivation (Joo et al., 2016). For example, studies have shown that artether treatment can increase the expression of p-MAPK and the phosphorylation of GSK3β at the Ser9 site in both Aβ1-42-treated PC12 neuronal cultures and in the brain cortex of 3xTg-AD mice and upregulate the expression of Nrf2 and HO-1, thereby triggering the activation of antioxidant and anti-inflammatory genes (Li et al., 2019). Casein kinase II (CK2) is a protein involved in replication, gene transcription and transduction that can phosphorylate Nrf2 for nuclear accumulation and the activation of Nrf2 (Pi et al., 2007; Apopa et al., 2008). p38 MAPK is a class of evolutionarily conserved serine/threonine MAPKs involved in the regulation of oxidative stress. p38 MAPK can phosphorylate Nrf2 at three serine residues (Ser215, Ser408 and Ser577), which promotes decreased Nrf2 nuclear accumulation (Sun et al., 2009). Studies have shown that an increased amyloid β load in AD leads to increased levels of p38 MAPK, which are accompanied by a decrease in total Nrf2 expression as well as a decrease in accumulated Nrf2 in the nucleus. Therefore, the levels of antioxidant enzymes, such as HO-1 and NQO1, which are regulated by Nrf2, were reduced (Zhang et al., 2021a).

SIRT1/Nrf2 pathway in ferroptosis

The balance of histone acetylation and deacetylation plays a key role in the regulation of gene expression. Lysine residues on the N-terminal of histone proteins acetylation induced by histone acetyl transferases (HATs) is associated with gene transcription, while histone deacetylation induced by histone deacetylase (HDAC) activity is associated with gene silencing (Khan et al., 2021). Acetylation or deacetylation of NRF2 by HATs and HDAC significantly changes its nuclear retention in neurons (Chang and Guarente, 2014).

Silent information regulator 1 (Sirtuin 1, SIRT1) is a class III deacetylase that is nicotinamide adenine dinucleotide (NAD+)-dependent and predominantly localized in the nucleus. SIRT1 can achieve epigenetic regulation by deacetylating lysine residues in histones. Recent studies have shown that the SIRT1/Nrf2 pathway is involved in neuroprotective effects. SIRT1-induced deacetylation promotes the nucleocytoplasmic localization and transcriptional activity of Nrf2, thereby increasing antioxidant capacity (Figure 4). A study showed that a low concentration of Aβ25-35 could increase the activity of SOD and the expression of SIRT1 and Nrf2 in the primary neurons and cortex of mice, indicating that a low concentration of Aβ25-35 could initiate antioxidant capacity by activating the SIRT1/Nrf2 pathway to induce autophagy (Zhu et al., 2021). Meanwhile, studies have also shown that vitamin D reduces oxidative stress by upregulating SIRT1, NRF-2 and HO-1 and downregulating NF-κB (Ali et al., 2021). These results indicate that increasing SIRT1 can activate the Nrf2 pathway through deacetylation by SIRT1 and trigger transcriptional activation of a series of downstream antioxidant enzymes to resist oxidative stress.

The p53/SLC7A11 pathway regulates ferroptosis

SLC7A11 is a functional light chain subunit of the Xc-system and together with the heavy chain subunit SLC3A2 constitutes system Xc-, which is critical for ferroptosis. SLC7A11 plays a crucial role in cystine uptake that subsequently prevents ferroptosis. p53 has been reported to downregulate the expression of SLC7A11 via transcriptional regulation and to induce the iron-dependent cell death process (Wang et al., 2020a). p53 is activated by acetylation at its lysine K101 site in humans (or K98 in mouse p53), thus promoting ferroptosis (Wang et al., 2016a; Chen et al., 2021b). A study confirmed that although ablation of p53 acetylation does not significantly affect p53 expression, it does significantly abrogate p53-mediated transcriptional regulation of targets such as SLC7A11.

In addition, p53 can be deacetylated by Sirt-1, inhibiting its function of suppressing transcriptional activation of SLC7A11 and further increasing the expression of SLC7A11, thereby inhibiting ferroptosis.

The AMPK/mTOR pathway regulates autophagy-dependent ferroptosis

AMPK is a regulator of autophagy through the mTOR signaling pathway, which can initiate chaperone-mediated autophagy and control cellular defenses against oxidative stress. mTOR is a mammalian target of rapamycin, a member of the PI3K-related kinase (PIKK) family with serine/threonine protein kinase activity. The phosphorylation of AMPK can phosphorylate mTOR to inhibit the activity of mTOR and suppress cell autophagy initiation (Ge et al., 2022). Autophagy-dependent ferroptosis is ferroptotic cell death triggered by autophagy, characterized by lipid peroxidation and iron accumulation (Liu et al., 2020). Recently, studies confirmed that the AMPK/mTOR pathway is involved in regulating autophagy-dependent ferroptosis. Inactivation of mTOR by phosphorylation of AMPK results in significantly elevated LC3B-II accumulation and beclin1 protein expression, thereby promoting autophagy and initiating autophagy-dependent ferroptotic cell death. The function of AMPK in the mediation of ferroptosis is required for beclin1 phosphorylation, with inhibition of system Xc-activity (Song et al., 2018).

Moreover, SIRT3 promotes the induction of autophagy-dependent ferroptosis by activating the AMPK-mTOR pathway. SIRT3 knockdown decreases AMPK phosphorylation, suppresses AMPK activation, and increases mTOR activation. SIRT3 silencing also significantly decreases LC3B-II accumulation and beclin1 protein expression, thereby suppressing autophagy. Depletion of SIRT3 decreases the phosphorylation level of AMPK, increases mTOR activity, and decreases LC3B-II and Beclin1 expression, eventually inhibiting autophagy activation and blocking the induction of ferroptosis (Han et al., 2020). In addition, depletion of SIRT3 inhibits ferroptosis by increasing GPX4 levels.

Potential ferroptosis-inhibiting drugs for the treatment of ADIron chelators

The iron chelators is a component with an affinity for iron ions, which can regulate the expression of iron-dependent proteins by chelating iron, thereby slowing down the pathological process of AD. Previous studies have reported several iron chelators applied in clinical studies, such as deferoxamine, minocycline and clioquinol. Unfortunately, deferoxamine and minocycline do not result in any clinically meaningful difference in the rate of decline of cognitive and functional ability, therefore, they are limited as therapeutics for AD. However, novel iron chelators are potential drugs for the treatment of AD.

[5-(N-methyl-N-propargylaminomethyl)-8-hydroxyquinoline] (M-30) is a synthesized iron chelator whose prototype is 8-hydroxyquinoline (VK-28). M-30 has propargyl monoamine oxidase (MAO) inhibitory neuroprotective and iron-chelating moieties, and it can penetrate the blood–brain barrier, which has demonstrated pro-survival neurorescue action and promoted neuronal differentiation and neuronal outgrowth. The role of M-30 may be due to its iron chelating and inhibition of membrane lipid peroxidation properties, which can prevent ferroptosis. A study showed that M-30 markedly reduced the levels of cellular APP and Aβ generation by promoting the nonamyloid pathway (Avramovich-Tirosh et al., 2007). In addition, the therapeutic efficacy of M30 for AD was also confirmed in an in vivo animal model. Oral pretreatment with M30 significantly prevented the development or ameliorated the already developed cognitive deficits in the STZ-induced rat model of sporadic AD. Chronic M30 treatment inhibited STZ-induced hyperphosphorylation of tau protein and decreased the expression of insulin-degrading enzyme (IDE) in the hippocampus. IDE, as a metalloprotease for the degradation of Aβ, was found to be reduced in sporadic AD (Salkovic-Petrisic et al., 2015). Therefore, M-30, as a multifunctional iron chelator drug, exhibits a potential therapeutic effect for sporadic AD.

Deferiprone is a clinically available iron chelator that recently determined the effectiveness for AD in an in vivo animal model. Deferiprone was determined to inhibit cognitive impairment in a scopolamine (SCOP)-induced AD rat model via an antioxidative effect (Fawzi et al., 2020). Pretreatment with deferiprone significantly attenuated the SCOP-induced increase in hippocampal and cortical acetylcholinesterase (AChE) activity in rats and decreased Aβ and iron deposition. In addition, deferiprone significantly reduced iron levels in the brain and further reduced p-tau by downregulating GSK3β and CDK-5 in the hippocampus of mice (Rao et al., 2020). This finding indicated that intervention with deferiprone may be effective in slowing disease progression in AD.

Alpha-lipoic acid (α-LA) is a naturally occurring enzyme cofactor with neuroprotective properties in AD due to its antioxidant and iron chelator properties. Earlier studies have shown that a-LA has the ability to slow cognitive decline in aged mice and AD mice, although it does not reduce Aβ levels or plaque deposition (Farr et al., 2003). However, in vitro experiments have verified that a-LA can inhibit the formation of Aβ fibrils (Ono et al., 2006). Further study showed that a-LA markedly inhibited the hyperphosphorylation of tau partly by enhancing the expression of protein phosphatase 2 A (PP2A), which could attenuate oxidative stress and ferroptosis in P301S Tau transgenic mice with tauopathy (Zhang et al., 2018).

Based on this evidence, the therapeutic effect of iron chelators on AD may be related to their ability to capture iron from Aβ and suppress Aβ aggregation (Opazo et al., 2006). Both in vitro and in vivo studies have confirmed that iron chelators can effectively alleviate ferroptosis and neuronal death caused by iron dyshomeostasis in AD and thus may be potential drugs for the treatment of AD in the clinic.

Lipid peroxidation inhibitors

Lipid peroxidation has been shown to be an early event in AD pathogenesis and related to ferroptosis. Dyshomeostasis of intracellular iron induces the production of ROS, driving ferroptosis in AD. The molecules that reduce lipid peroxidation can be used as potential drugs against AD due to their roles in suppressing the formation of lipid peroxides.

CMS121 is a small molecule and derivative of the flavonoid fisetin and may effectively alleviate AD by inhibiting lipid peroxidation. Currently, CMS121 alleviates cognitive dysfunction in APPswe/PS1ΔE9-transgenic mice. CMS121 (34 mg/kg/day)-treated AD mice prevent excess lipid peroxidation and reduce neuroinflammation in the brain. CMS121 treatment significantly decreased the toxic lipid peroxidation product 4HNE protein in the hippocampus compared with that in AD mice and prevented Aβ accumulation and inhibited cell death. FASN, as a key enzyme in the synthesis of lipids, is a target of CMS121. A previous study showed that FASN is increased in AD patients and promotes the production of 4HNE. CMS121 suppresses the level of FASN to protect against lipid peroxidation and neuroinflammation in neurodegeneration (Ates et al., 2020). Furthermore, CMS121 has a neuroprotective role by enhancing acetyl-CoA levels to target and inhibit acetyl-CoA carboxylase 1 (ACC1), exhibiting the effect of memory enhancement in SAMP8 mice (Currais et al., 2019).

Nrf2 agonists function as antioxidants

As a key regulator of antioxidant pathways and ferroptosis, Nrf2 exerts a critical role in AD. Some drugs including synthetic and natural compounds can promote Nrf2-mediated transcriptional regulation of the production of antioxidant enzymes and resist neuronal damage and ferroptosis caused by oxidative stress in AD. Some of the drugs currently used as Nrf2 agonists are synthetic compounds, but most are natural compounds. Regardless of the mechanism by which the compounds exert their effects, their main function is to act as antioxidants by activating the Nrf2/Keap1/HO-1 pathway.

Synthetic compounds

The recently reported synthetic Nrf2 agonists are dimethyl fumarate (DMF), Dl-3-n-butylphthalide (Dl-NBP) and benfotiamine (BFT), and their effectiveness in treating AD has been validated in vitro and in vivo (Table 1).

TABLE 1

TABLE 1. Summary of novel ferroptosis inhibitors that have been studied in AD.

DMF was previously shown to be a neuroprotective drug used in clinical trials on patients with relapsing-remitting multiple sclerosis. Currently, DMF is identified as a synthetic Nrf2 stimulator that has effects on antioxidant and inflammatory pathways, which might play a neuroprotective role against Aβ-induced cytotoxicity (Campolo et al., 2018). DMF inhibits ROS overproduction and reduces Aβ deposition, alleviates memory impairment and hippocampal atrophy in AD model mice and delays the progression of AD by activating the Nrf2 pathway (Sun et al., 2022). Furthermore, DMF also mitigates tauopathy in Aβ-treated cell models. DMF pretreatment suppressed tau phosphorylation at Ser396 and Aβ1-42-induced Thr231 by reducing the activity of GSK-3β in cells to inhibit NFT formation (Rajput et al., 2020).

Dl-NBP is a synthetic compound based on L-3-n-butylphthalide that is isolated from seeds of Apium graveolens. Dl-NBP has been identified as a potentially useful drug for the treatment of AD. Early, Dl-NBP was demonstrated to reduce the levels of soluble Aβ and Aβ oligomers in the APP/PS1 mouse brain (Wang et al., 2016b). A study showed that Dl-NBP promoted the cyclic adenosine monophosphate-response element-binding protein (CREB)–CREB-binding protein (CBP) interaction and increased CBP-mediated Nrf2 acetylation, therefore activating the Nrf2 signaling pathway, reducing ROS production and attenuating oxidative stress in the APP/PS1 mouse brain (Wang et al., 2016b). Dl-NBP was found to suppress neuroinflammation and reduce Aβ secretion in Alzheimer’s-like pathology (Wang et al., 2019). Nrf2 acts as a negative regulator of thioredoxin (TrX)-interacting protein (TXNIP) and can inhibit the expression of TXNIP at a low level, thereby inhibiting the activation of NLRP3 inflammasomes. Dl-NBP treatment inhibited TXNIP-TrX signaling by regulating Nrf2, further suppressing TXNIP, enhancing TrX activity and ameliorating neuronal apoptosis and neuroinflammation in APP/PS1 mouse brains (Wang et al., 2019). Furthermore, the utility of Dl-NBP in the treatment of AD has been studied preclinically. Applying donepezil combined with Dl-NBP in the treatment of mild to moderate AD showed that after 48 weeks of treatment with donepezil combined with NBP, cognitive decline was slower in patients with AD (Wang et al., 2021).

BFT is a synthetic liposoluble derivative of vitamin B1 (also called thiamine), which has better bioavailability than thiamine. Several studies have shown that BFT can rescue cognitive deficits and reduce Aβ burden in APP/PS1 mice and AD patients. For example, a clinical study showed that oral administration of 300 mg/day BFT for 18 months to five AD patients with mild-to-moderate dementia could improve cognitive decline, despite not eliminating brain amyloid accumulation (Pan et al., 2016). Furthermore, in the APP/PS1 mouse model, BFT improved cognitive function while suppressing glycogen synthase kinase-3 (GSK-3) activity, although it did not inhibit β-secretase (BACE) activity (Pan et al., 2010). Recently study showed that BFT treatment can reverse the reduction in SOD-2 activity as well as the decrease in PGC-1a levels by activating the Nrf2/ARE transcription pathway, further decreasing GSK-3β activity and diminishing tau phosphorylation (Tapias et al., 2018).

Natural compounds

A variety of natural compounds have been reported to act as Nrf2 agonists and have the potential to treat AD. Most of them are flavonoids (including genistein, quercetin, eriodictyol and 5,6,7,4′-tetramethoxyflavanone), polyphenols (including resveratrol and cyanidin), terpenoids (ginkgolide B) and other types of compounds. Regardless of the type of compound, their ultimate function is to promote Nrf2 expression and activation, thereby inducing the expression of antioxidant enzymes such as HO-1, NQO-1 and SOD. The specific compounds and their mechanism are shown in Table 1.

Flavonoids

Genistein is a natural isoflavone derived from soybean extract and has been reported to have anti-inflammatory and antioxidative activities. Genistein has been identified to potentially ameliorate learning and memory deficits in AD (Bagheri et al., 2011). Recently, it was determined that genistein exerts a neuroprotective effect on an AD cell model via Nrf2/HO-1/PI3K signaling. Genistein protects against the neurotoxicity induced by Aβ25-35 by upregulating PI3K p85 phosphorylation to activate Nrf2, which further enhances HO-1 expression. Furthermore, a study showed that genistein treatment reversed the Aβ25-35-induced decrease in alpha7 nicotinic acetylcholine receptor (α7nAChR) expression in hippocampal neurons and activated the PI3K/Akt/Nrf2/keap1 signaling cascade, indicating that genistein has therapeutic potential in the prevention and treatment of AD (Guo et al., 2021).

Quercetin is one of the more common plant-derived flavonoids with antioxidant properties. Recent studies have shown that quercetin acts as an antioxidant in AD cell models and animal models. Yu et al. determined that quercetin increased the expression of sirtuin1, Nrf2 and antioxidant enzymes, thereby acting as an antioxidant in Aβ25-35-induced PC12 cells (Yu et al., 2020). Quercetin also attenuated cholinergic dysfunction and cognitive impairments in STZ-induced AD-like dementia rats (Singh and Garabadu, 2021). Study showed that quercetin with 50 mg/kg treatment significantly reversed STZ-induced decreases in the levels of α7nAChRs and HO-1 in the rat brain, which improved cholinergic functions and abolished the accumulation of Aβ in the brains of rats subjected to STZ. This finding indicated that quercetin can be used as a potential therapeutic drug in the management of AD by regulating the α7nAChR/Nrf2/HO-1 signaling pathway. In addition, quercetin treatment can reduce tau aggregation and protected cells against tau neurotoxicity by mediating the expression of heat shock protein family B member 1 (HSPB1), NRF2, and tropomyosin-related kinase B (TRKB) (Chiang et al., 2021). Previous studies revealed that the levels of NRF2 can be upregulated by AKT serine/threonine kinase 1 (AKT), one of the downstream proteins activated by TRKB (Yoo et al., 2017). HSPB1 regulates the status of tau protein, which is a target of NRF2 (Sahara et al., 2007).

Eriodictyol is a natural flavonoid that is widely found in citrus fruits and some Chinese herbal medicines. Eriodictyol has been found to exert antioxidant and anti-inflammatory activity. Studies showed that eriodictyol induced the activation of the Nrf2/ARE signaling pathway, leading to the upregulation of Nrf2-dependent antioxidant capacity, thereby reducing Aβ-induced oxidative damage (Jing et al., 2015). Recently, a study by Li et al. further confirmed that eriodictyol ameliorates cognitive dysfunction in APP/PS1 mice by inhibiting ferroptosis via vitamin D receptor (VDR)-mediated Nrf2 activation (Li et al., 2022). Eriodictyol treatment obviously increased the expression of GPX4 and promoted the activation of the Nrf2/HO-1 signaling pathway in the cortex and hippocampus of APP/PS1 mice. However, the effect of eriodictyol on promoting GPX4 expression and the activation of the Nrf2/HO-1 signaling pathway were reversed by VDR knockout. The results suggest that eriodictyol inhibits ferroptosis by activating the Nrf2/HO-1 signaling pathway, thereby attenuating memory impairment in AD, and this process is mediated by VDR.

5,6,7,4′-tetramethoxyflavanone (TMF) is a flavonoid isolated from Chromolaena odorata (L.), which exerts antioxidation and anti-inflammation effects. Recently, TMF has been well demonstrated to have a function against neurodegeneration. For example, Jumnongprakhon et al. found that TMF attenuated Aβ25-35 toxicity by activating Nrf2 signaling and upregulating phase II antioxidative enzymes, further inhibiting NOX-4 activity and partially activating Sirt-1 (Jumnongprakhon et al., 2021). Pakdeepak et al. determined that TMF reduced DEX-induced Aβ generation by decreasing the expression of BACE1 and presenilin 1 (PS1) and increasing the expression of the a-secretase ADAM10. TMF also decreased pTau expression by inhibiting the activation of GSK-3. In addition, TMF also improved synaptic function by increasing the expression of Syn and PSD95 while decreasing acetylcholine esterase activity (Pakdeepak et al., 2020). These results suggest that TMF might have potential as a therapeutic drug for AD.

Polyphenols

Resveratrol, a natural polyphenol derived from grapes, has been widely reported to have diverse antioxidative effects against AD. Studies show that resveratrol treatment enhanced the nuclear translation and translation of Nrf2 in the brains of aged SAMP8 mice, and it enhanced SOD, glutathione peroxidase (GSH-Px), CAT activities and HO-1 protein levels and decreased MDA content, therefore decreased Aβ level and increased ChAT level (Kong et al., 2019). Moreover, Hui et al. determined that resveratrol activated the PI3K/Akt signaling pathway, further phosphorylating Nrf2 and promoting Nrf2 translocation into the nucleus, initiating gene transcription (Hui et al., 2018). The utility of resveratrol in AD treatment has also been validated in clinical trials. A 52-week trial of resveratrol treatment in mild-moderate AD patients significantly reduced CSF Aβ42 levels and neuroinflammation via the activation of SIRT1. Resveratrol treatment attenuated declines in mini-mental status examination (MMSE) scores and changes in ADL (ADCS-ADL) scores (Moussa et al., 2017).

Cyanidin is a natural polyphenolic pigment widely found in plants. Previously, studies have determined that cyanidin can suppress Aβ-induced neurotoxicity in an AD cell model (Wang et al., 2016c). A recent study showed that cyanidin inhibits the TLR4 pathway by increasing the translocation of Nrf2 into the nucleus and the activation of the Nrf2 pathway, further upregulating the production of HO-1, NQO-1, GCLC, and SOD and inhibiting the NF-κB pathway. Furthermore, cyanidin could prevent Aβ-induced oxidation and neuroinflammation by targeting the TLR4/NOX4 signaling pathway, indicated that cyanidin could be used as a promising compound to the treatment of AD (Thummayot et al., 2018).

Terpenoids

Ginkgolide B (GB) is one of the major active components derived from Ginkgo biloba extract and is widely used for the treatment of neurodegenerative diseases (Wang et al., 2020b). GB attenuates free radical damage and reduces inflammation, and it has a neuroprotective role in resisting ferroptosis in animal models of AD. Shao et al. determined that GB treatment for 4 weeks could effectively alleviate cognitive impairment by mitigating oxidative stress, neuroinflammation and ferroptosis in the brains of SAMP8 mice (Shao et al., 2021). GB treatment significantly reduced the Fe2+ content in the brains of SAMP8 mice. Moreover, GB treatment reversed the increased expression of TFR1 and NCOA4 and upregulated the expression of Nrf2 and GPX4 in the brains of SAMP8 mice, suggesting the involvement of Nrf2/GPX4 pathway-regulated ferroptosis in the GB-related protective effects on the AD mouse model.

Others

Tetrahydroxy stilbene glycoside (TSG) is the main active substance in Polygonum multiflorum. Recently, TSG has been beneficial in promoting learning and memory in AD and aged mouse models. TSG treatment enhanced the antioxidant capacity and reduced Aβ40/42 deposition in APP/PS1 mice by promoting the activation of GSH/GPX4/ROS and Keap1/Nrf2/ARE signaling pathways, thereby inhibiting neuronal cell death and ameliorating cognitive decline in AD mice (Gao et al., 2021).

Sulforaphane is an active component extracted from cruciferous vegetables and a pharmacological activator of Nrf2, which was identified as having neuroprotective functions. Early studies have shown that sulforaphane exerts antioxidative and anti-inflammatory effects by regulating Nrf2 expression and promoting Nrf2 nuclear translocation by decreasing the DNA methylation levels of the Nrf2 promoter in an AD cell model (Zhao et al., 2018). In vivo, sulforaphane treatment at 40 mg/kg ameliorated neuronal injury in the hippocampi and improved the learning and cognitive ability of AD rats. At the same time, sulforaphane significantly upregulated the contents of thioredoxin and glutathione as well as the activities of antioxidant enzymes, along with the level of Nrf2, and decreased the level of Aβ1-42 in the cortex, hippocampus and dentate gyrus of AD rats (Zhang et al., 2021b). Another study showed that sulforaphane promoted Nrf2 nuclear translocation and increased the levels of the antioxidases HO-1 and NQO1 to exert antioxidant defenses and protective effects on cognitive impairments (Pu et al., 2018). Both in vivo and in vitro experiments have demonstrated that sulforaphane exerts an antioxidant role in AD partially through activating the Nrf2 pathway.

Oxyphylla A is a novel phenolic acid compound extracted from Alpinia oxyphylla, which is known as Yi Zhi in Chinese. Oxyphylla A could decrease the expression of APP, Aβ1-40 and Aβ1-42 but increase the mRNA levels of HO-1 and NQO1 and promote Nrf2 translocation from the cytoplasm to the nucleus in N2a/APP cells. Treatment with 20 mg/kg Oxyphylla A increased the expression level of Nrf2 and its downstream targets, such as HO-1 and NQO1, in both the hippocampus and cortex of mice, as well as ameliorate cognitive deficits in old SAMP8 mice (Bian et al., 2021). Thus, Oxyphylla A might exert neuroprotective effects through the Nrf2/Keap1/HO-1 pathway.

Conclusion and perspectives

Understanding the complex pathophysiology of AD is a priority for discovering new therapeutic targets for AD and developing novel drugs. Studies have shown that the mechanism of ferroptosis is closely related to the pathogenesis of AD, and the potential key regulatory factors that trigger ferroptosis are also plays an important role in the pathological process of AD (Figure 5). Iron dyshomeostasis contribute to SP deposition and NFTs. Iron metabolism imbalance in brain and the dysfunction of endogenous antioxidant systems including system Xc- and GPX are closely related to the etiopathogenesis of AD. Dysfunction of NCOA4-mediated ferritinophagy induced ferroptosis can accelerates the pathological process of AD. Therefore, iron dyshomeostasis and ferroptosis could be possible targets for AD therapy. The role of Nrf2 as a critical regulator of antioxidant stress has attracted much attention in recent years. NRF2, through regulating the expression of a considerable