Acetylated tubulin Cytoplasm ependymal cell cilia Microtubule structure and function Sigma Aldrich Mouse Monoclonal Clone 6-11B-1 Cat. 6793 Comte et al. (2012); Kotagiri et al. (2014) ALDH1L1 Cytoplasm astrocytes Formate oxidation NeuroMab Mouse N103/39 Sorrells et al. (2018) Amyloid beta1 Extracellular filamentous protein deposit n/a Contributes to Alzheimer's pathology Chemicon Mouse Monoclonal MAB1560 Leonard et al. (2009) Chemicon (Millipore) Mouse Monoclonal MAB1561 Leonard et al. (2009) Signet Labs Mouse Monoclonal Clone 4G8 Perry et al. (2012) BioLegend/Covance Mouse Monoclonal 805503 Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Specific signal with 70% formic acid (Flor-García et al., 2020) Aquaporin 4 Cell membrane Ependymal cells Cell H2O intake Millipore Rabbit Polyclonal AB3594 Oreja-Guevara et al. (2017) Bcl (Cyclin D1) Nucleus Stem and progenitor cells Cell cycle progression Chemicon Rabbit Polyclonal ABE52 Kukekov et al. (1999) Beta-III-tubulin Cytoskeleton Newborn and young neurons Microtubule formation Sigma Mouse Monoclonal T8860 Kukekov et al. (1999); Curtis et al. (2003); Liu et al. (2008); Leonard et al. (2009); Knoth et al. (2010); Perry et al. (2012) Sigma Mouse Monoclonal T8578 Nait-Oumesmar et al. (2007) Sigma Mouse Monoclonal T5076 Höglinger et al. (2004); Van den Berge et al. (2011); van Strien et al. (2014) Covance—Eurogentec Mouse Monoclonal MMS-435P Blümcke et al. (2001) Absent signal specificity on post-mortem human brain (Moreno-Jiménez et al., 2019). Now sold as BioLegend 801,201. Abcam Mouse ab47967 Nogueira et al. (2014) Abcam Mouse Monoclonal ab78078 Oreja-Guevara et al. (2017) Millipore Chicken AB9354 Galán et al. (2017) Synaptic Systems Guinea Pig Polyclonal 302,304 Moreno-Jiménez et al. (2019) Variable signal specificity on post-mortem human brain (requires HC-AR NaBH4; Moreno-Jiménez et al., 2019) Promega Mouse Monoclonal G7121 Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) BLBP Cytoplasm, Nucleus Radial glia-like astrocytes–neural stem cells fatty acid uptake EMD Millipore Rabbit ABN14 Sorrells et al. (2018) Abcam Mouse ab131137 Sorrells et al. (2018); Moreno-Jiménez et al. (2019) Absent signal specificity on post-mortem human brain (Moreno-Jiménez et al., 2019) Abcam Rabbit Polyclonal ab32423 Moreno-Jiménez et al. (2019) Absent signal specificity on post-mortem human brain (Moreno-Jiménez et al., 2019) BMP6 Extracellular, Mitochondria. Secreted ligand Broad function in neural development Abgent Rabbit Polyclonal Crews et al. (2010) Millipore Mouse Monoclonal Crews et al. (2010) BrdU Nucleus Mitotic cells incorporate BrdU in S phase of cell cycle and retain it with no or few subsequent divisions n/a Boehringer Mouse Eriksson et al. (1998) Harlan Sera-Lab Rat Eriksson et al. (1998) Sigma Monoclonal Kukekov et al. (1999) Oxford Biotech Sanai et al. (2004) Dako Mouse Monoclonal M0744 Van den Berge et al. (2011) Calbindin Cytoskeleton, Cytoplasm, Nucleus interneurons, maturing interneurons Ca2 binding protein Oncogene Science Mouse Monoclonal Jin et al. (2004) Gift from Piers Emson Rabbit Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005); Liu et al. (2008) Chemicon Rabbit AB5054 Adorjan et al. (2017) Chemicon Chang et al. (2008); Sanai et al. (2012) Swant Rabbit Polyclonal CB38 Blümcke et al. (2001) Specific signal (Flor-García et al., 2020) Calretinin Cytoplasm Interneurons, young interneurons Ca2 binding protein Transduction Laboratories Mouse C69520 Knoth et al. (2010) Sigma Chang et al. (2008) Chemicon (Millipore) Rabbit Sanai et al. (2012); Gomez-Nicola et al. (2014) Synaptic Systems Guinea Pig Polyclonal 214,104 Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Good signal specificity on post-mortem human brain (Moreno-Jiménez et al., 2019). Specific signal (Flor-García et al., 2020) Swant Rabbit Polyclonal CR7697 Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005); Thom et al. (2005); Liu et al. (2008); Walton et al. (2012); Paredes et al. (2016); Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Signal specific (Flor-García et al., 2020) Caspase-3 Cytoplasm, Nucleus Apoptotic cells Pro-apoptotic BD Pharmingen Rabbit 559565 Jin et al. (2006) R&D Systems Rabbit AF835 Knoth et al. (2010) Millipore Rabbit Gomez-Nicola et al. (2014) Caspase-8 Cytoplasm, Nucleus Apoptotic cells pro-apoptotic, upstream of Caspase-3 Cell Signaling Mouse Monoclonal Jin et al. (2004) CD133 (Prominin1) Plasma membrane, Endoplasmic Reticulum NSC SGZ neurogenesis, Glioblastoma Abcam Rabbit ab66141 Nogueira et al. (2014) Abcam Rabbit Polyclonal ab16518 van Strien et al. (2014) CD271 (low-affinity nerve growth factor receptor) Plasma membrane, Nucleus NSC SVZ neurogenesis BD Biosciences Mouse Monoclonal 557194 van Strien et al. (2014) CD31 (PECAM-1) Plasma membrane Blood vessels Angiogenesis Abcam Rabbit Boldrini et al. (2012) CD34 Plasma membrane Blood ?, cell adhesion Immunotech Mouse Monoclonal Clone QBEND10 Macas et al. (2006) CD45 (Protein tyrosine phosphatase, receptor type, C) Plasma membrane, Nucleus All hematopoietic lineage cells Antigen receptor signaling Dako Mouse Monoclonal Clone 2B11+PD7/26 Blümcke et al. (2001) CD68 Golgi apparatus, Vesicles Microglia, macrophages ? Dako Mouse Monoclonal KP1 Blümcke et al. (2001) Abcam Mouse Monoclonal ab955 Oreja-Guevara et al. (2017) Dako Mouse Monoclonal Clone PG-M1 Thom et al. (2005); Macas et al. (2006); Nait-Oumesmar et al. (2007) ChAT (Choline-acetyltransferase) Cytoplasm, Nucleus Cholinergic neurons Acetylcholine synthesis Chemicon Rabbit Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) Chemicon Goat Polyclonal Perry et al. (2012) Complexin-II Cytoplasm, Nucleus, axon terminal Neurons Synaptic functions In-house Mouse Monoclonal LP27 Tobin et al. (2019) D1L Receptor Plasma membrane Dopamine receptor Increases cAMP Gift from B. Bloch Rabbit Höglinger et al. (2004) D2L Receptor Plasma membrane Dopamine receptor Decreases cAMP Gift from P. Vernier Rabbit Höglinger et al. (2004) DARPP32 Cytoplasm Striatal projection neurons Synaptic plasticity Cell Signaling Rabbit 2306S Ernst et al. (2014) Dopamine beta-hydroxylase Extracellular, Vesicles Noradrenergic neurons Metabolizes dopamine to norepinephrine Chemicon Rabbit Höglinger et al. (2004) Doublecortin (DCX) Cellular somata and processes Young neuroblasts Necessary for SVZ neuroblast cell migration Chemicon Guinea Pig Polyclonal AB5910 Quiñones-Hinojosa et al. (2006); Sanai et al. (2012) Replaced by Millipore AB2253. Previously shown to stain DCX-expressing glial cells in neurodegenerative disorders (Verwer et al. (2007)). Millipore Guinea Pig Polyclonal AB2253 Gomez-Nicola et al. (2014); Paredes et al. (2016); Sorrells et al. (2018); Boldrini et al. (2018); Cipriani et al. (2018); Flor-García et al. (2020) Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) Santa Cruz Mouse Monoclonal sc-271390 Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) Santa Cruz Goat Polyclonal sc-8066 Jin et al. (2004); Crespel et al. (2005); Boekhoorn et al. (2006); Jin et al. (2006); Liu et al. (2008); Crews et al. (2010); Knoth et al. (2010); Wang et al. (2011); Gomez-Nicola et al. (2014); Ekonomou et al. (2015); Dennis et al. (2016); Galán et al. (2017); Sorrells et al. (2018); Moreno-Jiménez et al. (2019); Tobin et al. (2019); Flor-García et al. (2020) One of the most popular and reliable neuroblast markers. No longer available. Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) Abcam Rabbit ab18723 Wang et al. (2011); Perry et al. (2012); Nogueira et al. (2014); Cipriani et al. (2018); Sorrells et al. (2018); Tobin et al. (2019); Flor-García et al. (2020) No specific signal (Flor-García et al., 2020) Cell Signaling Rabbit 4604S Liu et al. (2008); Maheu et al. (2015); Paredes et al. (2016); Sorrells et al. (2018) Sigma-Aldrich Rabbit SAB4500628 Boldrini et al. (2018) Atlas Rabbit Polyclonal HPA036121 Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) Gleeson Lab Rabbit Moreno-Jiménez et al. (2019); Flor-García et al. (2020) Specific signal with HC-AR and NaBH4 (Flor-García et al., 2020) Synaptic Systems Rabbit Polyclonal 326003 Flor-García et al. (2020) No specific signal (Flor-García et al., 2020) Synaptic Systems Guinea Pig Polyclonal 326004 Flor-García et al. (2020) No specific signal (Flor-García et al., 2020) Synaptic Systems Chicken Polyclonal 326006 Flor-García et al. (2020) No specific signal (Flor-García et al., 2020) Dynorphin Plasma membrane Striatal neuron subtype Pain modulator, diverse CNS functions Serotec Thom et al. (2005) EGFR Plasma membrane NSC, progenitors Proliferation, lineage progression Upstate Biotechnology Sheep Höglinger et al. (2004); Sanai et al. (2012) ENCAM (PSA-NCAM) Plasma membrane Neuroblasts neuroblast migration Chemicon Mouse Monoclonal Jin et al. (2006) ENK (enkephalin) Plasma membrane, ECM Subset of striatal projection neurons Opioid receptor ligand Sera Lab Mouse Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) Ferritin Cytoplasm ? Iron storage Sigma Rabbit Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) FGFR3 Plasma membrane Broad expression Ossification, cancer Santa Cruz Rabbit sc-9007 Donega et al. (2019) GABA ARa6 Plasma membrane ? ? Chemicon Rabbit AB5610 Knoth et al. (2010) GABA receptor alpha1 subunit (bd24) Plasma membrane Neurons GABA-ergic signaling Gift from Hanns Mohler Mouse Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) GABA receptor beta2, 3 subunit (bd17) Plasma membrane Neurons GABA-ergic signaling Gift from Hanns Mohler Mouse Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) GABA receptor gamma2 subunit Plasma membrane Neurons GABA-ergic signaling Gift from Hanns Mohler Guinea Pig Curtis, Penney, et al. (2005); Curtis, Waldvogel, et al. (2005) GAD65/67 Golgi apparatus, Vesicles Neuroblasts, GABAergic neurons Enzyme that converts glutamate to GABA Chemicon Rabbit AB1511 Chang et al. (2008); Knoth et al. (2010) Galactocerebroside Lysosome Oligodendrocytes ? Sigma Rabbit Polyclonal G9152 Leonard et al. (2009) Chemicon Rabbit Polyclonal AB142 Van den Berge et al. (2011) Chemicon Mouse Monoclonal MAB342 van Strien et al. (2014) GAPDH