Figure S1. Helical wheel diagram of TK9Z2 and TK9Z4 peptides generated using HeliQuest.

Figure S2. EDS element analysis can determine the composition ratio of the material. It can be confirmed that the self-assembled substances in the picture are TK9Z2(A) and TK9Z4(B).

Figure S3. SEM images of the TK9Z2 (A, B) and TK9Z4 (C, D).

Figure S4. Fluorescence emission spectra of the TK9Z2(A) and TK9Z4(B), Dissolved in PBS.

Figure S5. Fluorescence emission data of the TK9Z2 (A,C) and TK9Z4 (B,D).

Figure S6. (A) Helical wheel diagram of the TKZ1–3, TKZ2P4 generated using HeliQuest. (B) CD spectra of TKZ2P4 and overlap of TKZ2P4 and TKZ3 (50 μM).

Figure S7. Helical wheel diagram of the other antibacterial peptides generated using HeliQuest.

Figure S8. SEM images of TKZ2 and TKZ3 peptides. (A) TKZ2, (B) zoom of image A, TKZ2, (C) TKZ3, (D) zoom of image C, TKZ3.

Figure S9. EDS images of the TKZ2(A) and TKZ3(B).

Figure S10. Fluorescence emission spectra of the TKZ3 (A) and Control data MLKPEYIR (B), Dissolved in PBS. Ex = 258 nm.

Figure S11. Fluorescence data of the Control data (A) MLKPEYIR and (B) TKZ3.

Figure S12. HPLC profiles of synthetic peptides. HPLC conditions: linear gradient starting from 10 to 90% CH3CN in 0.1% aqueous TFA over 30 min at a flow rate of 1.0 mL/min, and detection at 220 nm.