In this article, Fig. 2B, TM4SF1 and β-actin are placed inversely in the results of U87 cells; and part of Fig. 4, the results 4D, E, F, G are missing and, the correct figures, Figs. 2 and 4 should have appeared as shown below.
Fig. 24,5-Dimethoxycanthin-6-one Inhibited the Expression of TSPAN1 and TM4SF1 in GSCs. (A) STRING database was used to predict the interaction between TSPAN1 and TM4SF1. (B) The expression of TM4SF1 and TSPAN1 was analyzed using Western blot after treatment with 4,5-dimethoxycanthin-6-one. (C) Docking of 4,5-dimethoxycanthin-6-one to TM4SF1 and TSPAN1, respectively. (D and E) The interaction between TM4SF1 and TSPAN1 was evaluated by Co-IP and IF. *P < 0.05 vs. GSC
Fig. 44,5-Dimethoxycanthin-6-one inhibited GBM cell proliferation and migration through the TSPAN1/TM4SF1 axis. (A and B) GBM cell proliferation was evaluated through CCK-8 and colony formation assays. (C and D) Wound-healing and transwell assays were utilized to determine cell migration and invasion ability. (E) U251 and U87 cell apoptosis were detected by flow cytometry. (F) The expression levels of cell cyclin-related genes CDK4 and CDK6 were analyzed using RTqPCR. (G) Flow cytometry was employed to assess cell cycle progression. *P < 0.05 vs. Control, #P < 0.05 vs. 4,5-Dimethoxycanthin-6-one, &P < 0.05 vs. oe-TSPAN1
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